BioTek BioSpa 8

Automated Incubator

Washing, media replacement and reagent injection

Full unattended workflow automation

Temperature, CO2/O2 control

Information

Automated incubator

The Agilent BioTek BioSpa 8 microplate automated incubator links Agilent BioTek readers and imagers together with washers and dispensers. This integration provides a full workflow automation of up to 8 microplates or other labware. The BioSpa 8 can automate your workflows, from ELISA to long-term live cell assays.

Temperature, CO₂/O₂ control, humidity monitoring, and lid handling ensure an ideal environment for cell cultures. BioSpa software records session timelines and environmental conditions and sends text and email alerts during long and short-term runs. Link a washer, dispenser, plate reader, or imager for complete workflow automation.

Live Cell Imaging:

Full unattended workflow automation for up to 8 microplates and other labware
Integrated temperature and CO2/O2 control, plus humidity monitoring
Fluorescence, high contrast brightfield, color brightfield and phase contrast imaging
OnDemand mode: Remove/replace labware, independent scheduling and multi-user profiles
Advanced cell-level analysis including nuclear and cytoplasmic masks
Washing, media replacement and reagent injection Automated Incubator
Complete workflow automation, sample prep to detection
Temperature, CO2/O2 control; humidity monitoring
Records environment conditions; automated alerts
OnDemand mode: Remove/replace labware, independent scheduling and multi-user profiles
Compact for biosafety cabinet use
Uncomplicated software and simple integration

How it works

Complete workflow automation, sample prep to detection

BioSpa 8 handles up to 8 microplates, Petri and cell culture dishes, and T25 flasks during automated processes, running for days to weeks. Place the labware in the BioSpa drawers, and the gripper moves to the connected imaging, detection, and liquid handlers as scheduled in the BioSpa software.

Records environment conditions, automated alerts

BioSpa software monitors and records the system and environment conditions throughout the entire run time. Customizable text and email notifications allow you to walk away from the process with confidence. OnDemand mode for simple, efficient imaging or detection protocols.

BioSpa Software controls all aspects of the BioSpa 8 Automated Incubator, including temperature and gas control and monitoring, along with humidity monitoring. The software features customizable text or email notifications and alerts, relieving the need for onsite monitoring. The software’s session timelines and environmental reports allow quick scrutiny of the process and system status. BioSpa software communicates with Gen5 and LHC software to control integrated imaging, detection and liquid handling instruments.

BioSpa Automated incubator with 8-plate capacity, incubation to 45 °C, with control and monitoring. Includes BioSpa software and one alignment kit.

BIOSPAG Automated incubator with 8-plate capacity, incubation to 45 °C and CO2/O2 control and monitoring, water pan and humidity monitoring. Includes BioSpa software and one alignment kit.

BioSpa Live Cell Imaging system can be comprised of of BioSpa 8 Automated Incubator, along with other compatible imagers, washers and dispensers:

• Cytation 5 Cell Imaging Multi-Mode Reader
• Cytation 1 Cell Imaging Multi-Mode Reader
• EL406 Washer Dispenser
• 405 LS Washer
• 405 TS Washer
• MultiFlo FX Multi-Mode Dispenser

GENERAL
Microplate types	6- to 1536-well standard height microplates, with or without lids.
Microplate types	Plate height range: 7.6 mm to 25.4 mm
Other labware supported	Cell culture dishes (60 mm and 35 mm), T25 flasks
Lidded plate handling	Robotic arm moves plate to and from BioSpa 8 and connected instrument; handles de-lidding and re-lidding plates.
Microplate capacity	Up to 8 microplates (or other labware)
Air filter	User-replaceable HEPA filter
Compatible instruments	Cytation 5, Cytation 3, Synergy Neo2, Synergy H1, Epoch 2, EL406, 405 TS, 405 LS, MultiFlo FX, MultiFlo
Temperature control	Range: to 45 °C
	Control resolution: 0.1 °C
	Uniformity + 0.5 °C at 37 °C
Software	BioSpa Automated Incubator Software:

	Provides programming interface for BioTek detection and liquid handling devices
	Allows user notification (text or email) of events and/or errors in the system
	Provides control, monitoring and logging of:
	CO2/O2
	incubator temperature
	Provides humidity level monitoring and logging
	Allows uninterrupted runs lasting days or weeks
	BioSpa Session mode for all imaging, detection and liquid handling operations
	BioSpa OnDemand mode for simplified, intuitive imaging- or detection-only workflows for single or multiple users
CO2 and O2 control (option)	Range: 0 – 20% (CO2); 1 – 19% (O2)
	Control Resolution: +0.1% (CO2 and O2)
	Stability: +0.2% at 5% CO2; +0.2% at 1% O2
Humidity control	rH: 80 to 95% (lidded plates and 5% CO2)
	Source: Removable water pan
	Water level sensor: Low water level alert
IMAGING SYSTEM
Imaging mode	Fluorescence
	Brightfield
	Color Brightfield
	Phase Contrast
Imaging method	Single color, multi-color, montage, time lapse, z-stacking
Image processing	Z-projection, digital phase contrast, stitching
Camera	16-bit gray scale, Sony CCD, 1.25 megapixel. 0.3 µm/pixel at 20x
Objective capacity	6 user-replaceable objectives
Objectives available	1.25x, 2.5x, 4x, 10x, 20x, 40x, 60x
Phase objectives available	4x, 10x, 20x, 40x
Image filter cube capacity	4 user-replaceable fluorescence cubes plus brightfield channel
Imaging filter cubes available	DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7,Acridine Orange (ACR OR), CFP-YFP FRET, propidium,Iodide, chlorophyll, phycoerythrin, CY5.5, TagBFP, Alexa568, Ex377 / Em647
Imaging LED cubes available	365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm
Automated functions	User-trained autofocus, autofocus, autoexposure, auto-LED intensity
Autofocus method	Image-based autofocus
	User-trained autofocus
	Laser autofocus (option)
Liquid handling	See the individual product pages at EL406, MultiFlo FX and 405 TS

Complete workflow automation, sample prep to detection BioSpa8 handles up to 8 microplates, Petri and cell culture dishes and T25 flasks during automated processes, running up to 2 weeks. Place the labware in BioSpa’s drawers, and the gripper moves to the connected imaging, detection and liquid handlers as scheduled in the BioSpa software.
Temperature, CO2/O2 control; humidity monitoring Critical physiological conditions are maintained in BioSpa: Incubation to 45 °C, CO2/O2 control and humidity monitoring facilitate live cell processes. BioSpa software continuously records the environment conditions to ensure assay success.
Records environment conditions; automated alerts BioSpa software monitors and records the system and environment conditions throughout the entire runtime. Customizable text and email notifications allow you to walk away from the process with confidence.
OnDemand Mode For simple, efficient imaging or detection protocols, OnDemand mode allows labware to be added or removed at any time, without interrupting operations in process. Multiple users can be defined in the system and each user can visually track their own experiment’s progress on the dynamic timeline.
Compact for benchtop use, or for optional biosafety cabinet use When the workflow calls for sterile conditions, BioSpa and integrated instruments is compact enough to fit in a biosafety enclosure.
Uncomplicated software and simple integration BioSpa software integrates seamlessly with Gen5 and LHC software to provide easy and intuitive scheduling for short and long-term processes.

Related instruments

BioTek Cytation C10

Confocal and widefield Imaging Reader. Combines spinning disk confocal and automated digital widefield microscopy, plus a multi-mode microplate reader.

Water-immersion objectives to capture more light with lower exposure times

Benchtop confocal imaging for every laboratory

Facilitate live cell imaging with environmental controls

BioTek 405 TS

The gold standard in microplate washing with clog detection

Self-maintaining design prevents clogging and failure in the plate washer

Hands-off, self-testing technology

BioTek Cytation 5

Cell Imaging Multi-Mode Reader. Combine automated digital widefield microscopy with conventional multi-mode microplate reading.

Combined digital microscopy and multi-mode detection

100 mW Alpha Laser

Label-free cell counting for cell proliferation studies

Information

Automated incubator

Live Cell Imaging:

How it works

Complete workflow automation, sample prep to detection

Records environment conditions, automated alerts

Related instruments

Recently viewed instruments

Newsletter

BioSPX

Our Businesses

The Netherlands

Belgium