EN 
Cytation C10
Information
The Cytation C10 Confocal Imaging Reader combines spinning disk confocal and automated digital widefield microscopy, plus a multi-mode microplate reader. This allows for an improved imaging quality and better cell analysis in a cost-effective manner. The C10 follows up on the previous Cytation™ equipment and is developed through gained expertise and user-feedback from the past models. Along with the Gen5 software, the Cytation™ C10 provides an easy and automated workflow from image capture, processing, data analysis to publishing.
- Combined spinning disk confocal, widefield microscopy and optional multi-mode microplate reader in one compact platform!
- High quality optical components
- Hit-picking: Multi-mode detection + imaging saves time and data storage
- Variable bandwidth monochromators for sensitivity and specificity
- Maintaining a consistent environment through CO2/O2 and temperature control
Variety of applications from endpoint measurements to live cell imaging:
- Cell viability/toxicity assays
- 3D Cell Culture Assays
- Cell Migration and Invasion Assays
- Cell Proliferation
- Label-free cell counting for cell proliferation studies
- Cell Metabolism imaging and normalization – Seahorse data
- Genotoxicity
- Plaque assay
- Fluorescent Microneutralization Assay
Configurations
| Part # | C10PHC2 | C10MPHC2 | C10PWC | C10MPWC | C10PW | C10MPW |
| Confocal 60 µm spinning disk | ||||||
| Confocal 40 µm spinning disk | ||||||
| Hamamatsu sCMOS camera | ||||||
| Sony CMOS camera | ||||||
| Multi-mode reading | ||||||
| Phase contrast |
Technical details
| GENERAL | |
| Detection modes | UV-Vis absorbance |
| Fluorescence intensity | |
| Luminescence | |
| Read methods | Endpoint, kinetic, spectral scanning, well area scanning |
| Microplate types | Monochromator: 6- to 384-well plates |
| Imaging: 6- to 1536-well plates | |
| Other labware supported | Microscope slides, Petri and cell culture dishes, cell culture flasks (T25), counting chambers (hemocytometer), Take3 Micro-volume plates |
| Temperature control | 4-Zone incubation to 45 °C with Condensation Control |
| Shaking | Linear, orbital, double-orbital |
| Software | Gen5 Microplate Reader and Imager Software included |
| Gen5 Secure for 21 CFR Part 11 compliance (option) | |
| Gen5 Image+ and Image Prime software available for full image analysis (option) | |
| Automation | BioStack and 3rd party automation compatible |
| CO2 and O2 control (option) | Range: 0 – 20% (CO2); 1 – 19% (O2), with optional Gas Controller |
| Models for both CO2 and O2 or CO2 only are available | |
| IMAGING – CONFOCAL MICROSCOPE | |
| Imaging modes | Fluorescence |
| Image processing | Z-projection, digital phase contrast, stitching |
| Camera | Hamamatsu Orca sCMOS, 16-bit grayscale camera or |
| Sony CMOS 16-bit grayscale camera | |
| Objective capacity | 6-position automated turret for user-replaceable objectives |
| Objectives available | 20x, 40x, 60x |
| Image filter cube capacity | 4 user-replaceable fluorescence cubes |
| Imaging filter cubes available | CFP, CY5, DAPI, GFP, RFP, TRITC, brightfield |
| Laser | 6-line |
| Automated functions | Autofocus, user-trained autofocus, autoexposure, auto-LED intensity |
| Autofocus method | Image-based autofocus |
| User-trained autofocus | |
| Laser autofocus (option) | |
| Positional controls | Software Control |
| Joystick controller (option) | |
| Imaging methods | Single color, multi-color, time lapse, montage, z-stacking, z-stack montage |
| Field of view | Hamamatsu sCMOS: 0.65 mm + 5% at 20x magnification |
| Sony CMOS: 0.70 mm + 5% at 20x magnification | |
| Image collection rate | Laser autofocus, 0 ms delay, 96 wells: 8 mins, 9 secs |
| Software autofocus, 0 ms delay, 96 wells: 12 mins, 1 sec | |
| IMAGING – WIDEFIELD MICROSCOPE | |
| Imaging mode | Fluorescence, phase contrast, color brightfield, user-selectable brightfield/high contrast brightfield |
| Imaging method | Single color, multi-color, time lapse, montage, z-stacking, z-stack montage |
| Image processing | Z-projection, digital phase contrast, stitching |
| Camera | Hamamatsu Orca sCMOS, 16-bit grayscale camera or |
| Sony CMOS 16-bit grayscale camera | |
| Objective capacity | 6-position automated turret for user-replaceable objectives |
| Objectives available | 1.25x, 2.5x (2.25x eff), 4x,10x, 20x, 40x, 60x |
| Phase objectives available | 4x, 10x, 20x, 40x |
| Image filter cube capacity | 4 user-replaceable fluorescence cubes, plus brightfield channel |
| Imaging filter cubes available | DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7, Acridine Orange, CFP-FRET, CFP-YFP FRET, Chlorophyll, Phycoerythrin (PE), Propidium Iodide, CY5.5, TagBFP, Tag BFP-FRET, GFP (Ex)-CY5 (Em), RFP (Ex)-CY5 (Em), Alexa 568, Ex 377/Em 647, Oxidized roGFP2, TRITC |
| Imaging LED cubes available | 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 554 nm, 590 nm, 623 nm, 655 nm, 740 nm |
| Automated functions | Autofocus, autoexposure, auto-LED intensity |
| Autofocus method | Image-based autofocus |
| User-trained autofocus | |
| Laser autofocus (option) | |
| Positional controls | Software Control |
| Joystick controller (option) | |
| Image collection rate | Image-based autofocus: |
| 96 wells, 1 color (DAPI), 4x, 6 minutes | |
| Laser autofocus: | |
| 96 wells, 1 color (DAPI), 4x, <3 minutes | |
| Image Analysis Software option | Gen5 Image+: Image analysis |
| Gen5 Image Prime: Advanced image analysis | |
| Gen5 Secure: 21 CFR Part 11 compliant features | |
| FLUORESCENCE INTENSITY | |
| Light source | Xenon flash |
| Detector | PMT |
| Wavelength selection | Quad monochromators (top/bottom) |
| Wavelength range | 250 – 700 nm (900 nm option) |
| Monochromator bandwidth | Variable, from 9 nm to 50 nm in 1 nm increments |
| Dynamic range | 7 decades |
| Reading speed (kinetic) | 96 wells, sweep mode: 10 seconds |
| 384 wells, sweep mode: 20 second | |
| LUMINESCENCE | |
| Wavelength range | 300 – 700 nm |
| Dynamic range | >6 decades |
| ABSORBANCE | |
| Light source | Xenon flash |
| Detector | Photodiode |
| Wavelength selection | Monochromator |
| Wavelength range | 230 – 999 nm, 1 nm increments |
| Monochromator bandwidth | 4 nm (230 – 285 nm), 8 nm (>285 nm) |
| Dynamic range | 0 – 4.0 OD |
| Resolution | 0.0001 OD |
| Pathlength correction | Yes |
| Monochromator wavelength accuracy | + 2 nm |
| Monochromator wavelength repeatability | + 0.2 nm |
| OD accuracy | <1% at 3.0 OD |
| OD linearity | <1% from 0 to 3.0 OD |
| OD repeatability | <0.5% at 2.0 OD |
| Stray light | 0.03% at 230 nm |
| Reading speed (kinetic) | 96 wells: 10 seconds |
| 384 wells: 20 seconds | |
| REAGENT INJECTORS (OPTION) | |
| Supported detection modes | All modes |
| Number | 2 syringe pumps |
| Supported labware | 6- to 384-well plates, Petri and cell culture dishes |
| Dead volume | 1.1 mL, with back flush |
| Dispense volume | 5 – 1000 µL in 1 µL increments |
| Plate geometry | 6- to 384-well microplates |
| Dispense accuracy | +1 µL or 2% |
| Dispense precision | < 2% at 50 – 200 µL |
| PHYSICAL CHARACTERISTICS | |
| Power | Instrument: External 250W (minimum), 24VDC power supply, compatible with 100-240 VAC @50-60Hz. |
| Optional 6-channel laser light source: External 250W power supply, compatible with 100- 240VAC @ 50-60Hz. | |
| Optional Hamamatsu scientific camera: External 75W power supply, compatible with 100- 240VAC @ 50-60Hz. | |
| Dimensions | 27″ W x 18.5″ H x 20″ D |
| (68.6 cm x 45.72 cm x 50.8 cm) | |
| Weight | 122 lbs (53.3 Kg) |
| REGULATORY | |
| Regulatory | CE and TUV marked. RoHS Compliant. Models for In Vitro Diagnostic use are available. |
Gas Controler
Gas Controller for CO2 and O2 controll
Increasingly, life science assays are run on live cells. Some of these assays require control over CO2 and O2 concentrations to modulate the environment for pH buffering or to create a hypoxic condition. These assays are traditionally run-in flasks in a low-throughput manner, but there is a burgeoning interest in automation of live cell-based assays in microplates for higher throughput and efficiency, therefore there is a need for cell-friendly microplate instrumentation.
The Gas Controller allows full control over CO2 and O2 concentrations to regulate the environment for microplate based live cell assays.
Gen 5 Software for Imaging & Microscopy
From whole organism imaging to high magnification subcellular image analysis, Gen5 helps transform raw data and images into meaningful results. Gen5 Software automates image capture from Agilent’s Lionheart Automated Microscope and Cytation Cell Imaging Multi-Mode Reader, and automates image processing and analysis to produce publication-ready images and quantitative data. This process of Augmented Microscopy™ enables simple, straightforward imaging workflows.
• Automated image capture
• Exceptional image processing tools
• Powerful image analysis
• Publication-ready images, graphs and data
Features
Augmented Microscopy automates image capture for samples with powerful tools for endpoint and time lapse workflows. Fluorescence, brightfield, color brightfield and phase contrast images are captured in slides, microplates, chamber slides, cell culture dishes for quantitative and qualitative analysis.
Gen5’s image processing tools provide exceptional processing capability to facilitate the analysis of complex biologies, including 3D samples, large montaged samples and processes including live cell kinetics. From easy brightness/ contrast adjustments to deconvolution and automated movie file creation, image processing in Gen5 enables optimized visualization and analysis for many applications. The independent optical paths ensure uncompromised performance in all modes
Image analysis tools in Gen5 cover a very broad range of application requirements, and are both powerful and easy to use. Analysis functions in Gen5 extend to quantitative data as well. Cell count, confluence, signal translocation, subpopulation analysis and kinetic analysis are just some of the powerful data provided by Gen5’s image analysis tools.
Augmented Microscopy tools include the ability to create publication-ready images, graphs and data using the functions in Gen5 software. There is no need to export images or data to external software to create scatterplots, histograms, EC50 graphs and other data displays for publication.
Features
Combined confocal and widefield microscopy
Image quality is considerably improved due to the combination of spinning disk confocal and widefield microscopy. The degree of detail is exceptionally enhanced, and this results in improved quantification and analysis of the assay, combined with the Gen5 imaging Software.
High quality optical components
To enable high quality images and publication-ready images, high standard objectives and filters are compatible with the Cytation C10. These high quality objectives and filters include the Hamamatsu sCMOS Orca, Semrock filters, Olympus objectives and other known brands.
Hit-picking: Multi-mode detection + imaging saves time and data storage
The plate reader screens all wells and identifies the wells that meet certain criteria. Only the wells that meet these, will be imaged by the microscope. This results in the saving of time and computer memory!
(1) Plate reader quickly identifies GFP positive wells.
(2) Only GFP positive wells are imaged, saving both time and computer memory.
The bandwidths of the monochromators in the Cytation C10 fluorescence detection module is selectable from 9 nm to 50 nm, in 1 nm increments. Variable bandwidths allow you to optimize detection conditions for fluorophores that have unique excitation or emission parameters, for example, a narrow Stokes shift. Adjusting the bandwidth results in better performance than conventional monochromators.
The plate reader optics of Cytation use a quad monochromator design with variable bandwidth. The bandwidth can be set anywhere between 9 and 50 nm in 1 nm increment. Large bandwidth settings (1) provide increased sensitivity and lower limits of detection. Small bandwidth settings (2) provide increased specificity when multiple signals are present, which reduces signal crosstalk and enhances assay performance
Cytation C10 is well suited for live cell assays, with temperature control to 45 °C, plus linear and orbital shaking. A gas controller module is available to help regulate CO2 and O2 levels in the system for longer-term live cell requirements. A dual reagent injector provides quick inject/image capability for observing fast reactions.
Successful live cell kinetic imaging relies on a consistent environment, including temperature control and CO2/O2 control and monitoring. Cytation C10 provides the perfect environment to grow and analyze live cells over time. Powerful movie maker and kinetic analysis software tools allow visualizing and analysis time-lapse experiments.
It’s fast and convenient to count live cells using the available high contrast brightfield option. Higher contrast allows a direct and label-free method for object identification using Gen5 software to count cells and characterize cell proliferation in kinetic studies.