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BioTek Cytation C10

Short product description
Confocal and widefield Imaging Reader. Combines spinning disk confocal and automated digital widefield microscopy, plus a multi-mode microplate reader.


The Cytation C10 Confocal Imaging Reader combines spinning disk confocal and automated digital widefield microscopy, plus a multi-mode microplate reader. This allows for an improved imaging quality and better cell analysis in a cost-effective manner. The automated water-immersion objectives capture more light, driving lower exposure times and reducing phototoxic impacts on live cells. Along with the Gen5 software, the Cytation™ C10 provides an easy and automated workflow from image capture, processing, data analysis to publishing.

Cytation C10 also includes widefield fluorescence, brightfield, and phase contrast optics. Onboard environmental controls enable live cell assays. Multiplate live cell assays are accomplished with Cytation C10 integrated with the Agilent BioTek BioSpa 8 automated incubator. Cytation C10 brings high-performance, affordable confocal to every laboratory.

  • Cytation C10 offers affordable, benchtop confocal imaging for every laboratory
  • Confocal microscopy enables improved image quality and analysis, revealing a level of detail in samples that is not possible with widefield optics
  • High-quality optical components allow the capture of stunning, publication-quality images
  • Water-immersion objectives to capture more light with lower exposure times
  • Combination of spinning disk confocal and widefield imaging plus multimode reader, allows the Cytation C10 to be ready for any assay with a modular, upgradable design
  • An available 60 µm deep sectioning disk (DSD) allows a clear view into thicker samples
  • Widefield imaging allows for faster acquisition of large samples at lower magnification, while confocal images small intracellular details or 3D samples, and both modes together provide highly multiplexed, multiparameter imaging experiments
  • Environmental controls, including incubation and CO2/O2 control and monitoring, facilitate live cell imaging
  • Imaging plus multimode detection and the hit-picking function quickly prescreen the microplate with the plate reader optics, then automatically image the samples that meet your hit criteria, saving time and data storage capacity
  • Variable bandwidth, quad monochromator plate reader optics enhance assay performance, including increased sensitivity and specificity
  • Combination of confocal and widefield imaging with multimode detection increases productivity with the ability to be ready for any application


Variety of applications from endpoint measurements to live cell imaging:

  • Cell viability/toxicity assays
  • 3D Cell Culture Assays
  • Cell Migration and Invasion Assays
  • Cell Proliferation
  • Label-free cell counting for cell proliferation studies
  • Cell Metabolism imaging and normalization – Seahorse data
  • Genotoxicity
  • Plaque assay
  • Fluorescent Microneutralization Assay



Confocal 60 µm spinning disk
Confocal 40 µm spinning disk
Hamamatsu sCMOS camera
Sony CMOS camera
Multi-mode reading
Phase contrast
  • Technical details

    Detection modesUV-Vis absorbance
    Fluorescence intensity
    Read methodsEndpoint, kinetic, spectral scanning, well area scanning
    Microplate typesMonochromator: 6- to 384-well plates
    Imaging: 6- to 1536-well plates
    Other labware supportedMicroscope slides, Petri and cell culture dishes, cell culture flasks (T25), counting chambers (hemocytometer), Take3 Micro-volume plates
    Temperature control4-Zone incubation to 45 °C with Condensation Control
    ShakingLinear, orbital, double-orbital
    SoftwareGen5 Microplate Reader and Imager Software included
    Gen5 Secure for 21 CFR Part 11 compliance (option)
    Gen5 Image+ and Image Prime software available for full image analysis (option)
    AutomationBioStack and 3rd party automation compatible
    CO2 and O2 control (option)Range: 0 – 20% (CO2); 1 – 19% (O2), with optional Gas Controller
    Models for both CO2 and O2 or CO2 only are available
    Imaging modesFluorescence
    Image processingZ-projection, digital phase contrast, stitching
    CameraHamamatsu Orca sCMOS, 16-bit grayscale camera or
    Sony CMOS 16-bit grayscale camera
    Objective capacity6-position automated turret for user-replaceable objectives
    Objectives available20x, 40x, 60x
    Image filter cube capacity4 user-replaceable fluorescence cubes
    Imaging filter cubes availableCFP, CY5, DAPI, GFP, RFP, TRITC, brightfield
    Automated functionsAutofocus, user-trained autofocus, autoexposure, auto-LED intensity
    Autofocus methodImage-based autofocus
    User-trained autofocus
    Laser autofocus (option)
    Positional controlsSoftware Control
    Joystick controller (option)
    Imaging methodsSingle color, multi-color, time lapse, montage, z-stacking, z-stack montage
    Field of viewHamamatsu sCMOS: 0.65 mm + 5% at 20x magnification
    Sony CMOS: 0.70 mm + 5% at 20x magnification
    Image collection rateLaser autofocus, 0 ms delay, 96 wells: 8 mins, 9 secs
    Software autofocus, 0 ms delay, 96 wells: 12 mins, 1 sec
    Imaging modeFluorescence, phase contrast, color brightfield, user-selectable brightfield/high contrast brightfield
    Imaging methodSingle color, multi-color, time lapse, montage, z-stacking, z-stack montage
    Image processingZ-projection, digital phase contrast, stitching
    CameraHamamatsu Orca sCMOS, 16-bit grayscale camera or
    Sony CMOS 16-bit grayscale camera
    Objective capacity6-position automated turret for user-replaceable objectives
    Objectives available1.25x, 2.5x (2.25x eff), 4x,10x, 20x, 40x, 60x
    Phase objectives available4x, 10x, 20x, 40x
    Image filter cube capacity4 user-replaceable fluorescence cubes, plus brightfield channel
    Imaging filter cubes availableDAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7, Acridine Orange, CFP-FRET, CFP-YFP FRET, Chlorophyll, Phycoerythrin (PE), Propidium Iodide, CY5.5, TagBFP, Tag BFP-FRET, GFP (Ex)-CY5 (Em), RFP (Ex)-CY5 (Em), Alexa 568, Ex 377/Em 647, Oxidized roGFP2, TRITC
    Imaging LED cubes available365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 554 nm, 590 nm, 623 nm, 655 nm, 740 nm
    Automated functionsAutofocus, autoexposure, auto-LED intensity
    Autofocus methodImage-based autofocus
    User-trained autofocus
    Laser autofocus (option)
    Positional controlsSoftware Control
    Joystick controller (option)
    Image collection rateImage-based autofocus:
    96 wells, 1 color (DAPI), 4x, 6 minutes
    Laser autofocus:
    96 wells, 1 color (DAPI), 4x, <3 minutes
    Image Analysis Software optionGen5 Image+: Image analysis
    Gen5 Image Prime: Advanced image analysis
    Gen5 Secure: 21 CFR Part 11 compliant features
    Light sourceXenon flash
    Wavelength selectionQuad monochromators (top/bottom)
    Wavelength range250 – 700 nm (900 nm option)
    Monochromator bandwidthVariable, from 9 nm to 50 nm in 1 nm increments
    Dynamic range7 decades
    Reading speed (kinetic)96 wells, sweep mode: 10 seconds
    384 wells, sweep mode: 20 second
    Wavelength range300 – 700 nm
    Dynamic range>6 decades
    Light sourceXenon flash
    Wavelength selectionMonochromator
    Wavelength range230 – 999 nm, 1 nm increments
    Monochromator bandwidth4 nm (230 – 285 nm), 8 nm (>285 nm)
    Dynamic range0 – 4.0 OD
    Resolution0.0001 OD
    Pathlength correctionYes
    Monochromator wavelength accuracy+ 2 nm
    Monochromator wavelength repeatability+ 0.2 nm
    OD accuracy<1% at 3.0 OD
    OD linearity<1% from 0 to 3.0 OD
    OD repeatability<0.5% at 2.0 OD
    Stray light0.03% at 230 nm
    Reading speed (kinetic)96 wells: 10 seconds
    384 wells: 20 seconds
    Supported detection modesAll modes
    Number2 syringe pumps
    Supported labware6- to 384-well plates, Petri and cell culture dishes
    Dead volume1.1 mL, with back flush
    Dispense volume5 – 1000 µL in 1 µL increments
    Plate geometry6- to 384-well microplates
    Dispense accuracy+1 µL or 2%
    Dispense precision< 2% at 50 – 200 µL
    PowerInstrument: External 250W (minimum), 24VDC power supply, compatible with 100-240 VAC @50-60Hz.
    Optional 6-channel laser light source: External 250W power supply, compatible with 100- 240VAC @ 50-60Hz.
    Optional Hamamatsu scientific camera: External 75W power supply, compatible with 100- 240VAC @ 50-60Hz.
    Dimensions27″ W x 18.5″ H x 20″ D
    (68.6 cm x 45.72 cm x 50.8 cm)
    Weight122 lbs (53.3 Kg)
    RegulatoryCE and TUV marked. RoHS Compliant. Models for In Vitro Diagnostic use are available.
  • Gas Controler

    Gas Controller for CO2 and O2 controll
    Increasingly, life science assays are run on live cells. Some of these assays require control over CO2 and O2 concentrations to modulate the environment for pH buffering or to create a hypoxic condition. These assays are traditionally run-in flasks in a low-throughput manner, but there is a burgeoning interest in automation of live cell-based assays in microplates for higher throughput and efficiency, therefore there is a need for cell-friendly microplate instrumentation.

    The Gas Controller allows full control over CO2 and O2 concentrations to regulate the environment for microplate based live cell assays.

  • Gen 5 Software for Imaging & Microscopy

    From whole organism imaging to high magnification subcellular image analysis, Gen5 helps transform raw data and images into meaningful results. Gen5 Software automates image capture from Agilent’s Lionheart Automated Microscope and Cytation Cell Imaging Multi-Mode Reader, and automates image processing and analysis to produce publication-ready images and quantitative data. This process of Augmented Microscopy™ enables simple, straightforward imaging workflows.

    • Automated image capture
    • Exceptional image processing tools
    • Powerful image analysis
    • Publication-ready images, graphs and data



    Augmented Microscopy automates image capture for samples with powerful tools for endpoint and time lapse workflows. Fluorescence, brightfield, color brightfield and phase contrast images are captured in slides, microplates, chamber slides, cell culture dishes for quantitative and qualitative analysis.

    Exceptional Image Processing Tools
    Gen5’s image processing tools provide exceptional processing capability to facilitate the analysis of complex biologies, including 3D samples, large montaged samples and processes including live cell kinetics. From easy brightness/ contrast adjustments to deconvolution and automated movie file creation, image processing in Gen5 enables optimized visualization and analysis for many applications. The independent optical paths ensure uncompromised performance in all modes
    Powerful Image Analysis
    Image analysis tools in Gen5 cover a very broad range of application requirements, and are both powerful and easy to use. Analysis functions in Gen5 extend to quantitative data as well. Cell count, confluence, signal translocation, subpopulation analysis and kinetic analysis are just some of the powerful data provided by Gen5’s image analysis tools.
    Publication-ready Images, Graphs and Data
    Augmented Microscopy tools include the ability to create publication-ready images, graphs and data using the functions in Gen5 software. There is no need to export images or data to external software to create scatterplots, histograms, EC50 graphs and other data displays for publication.
  • Features

    Combined confocal and widefield microscopy
    Image quality is considerably improved due to the combination of spinning disk confocal and widefield microscopy. The degree of detail is exceptionally enhanced, and this results in improved quantification and analysis of the assay, combined with the Gen5 imaging Software.


    High quality optical components
    To enable high quality images and publication-ready images, high standard objectives and filters are compatible with the Cytation C10. These high quality objectives and filters include the Hamamatsu sCMOS Orca, Semrock filters, Olympus objectives and other known brands.


    Hit-picking: Multi-mode detection + imaging saves time and data storage
    The plate reader screens all wells and identifies the wells that meet certain criteria. Only the wells that meet these, will be imaged by the microscope. This results in the saving of time and computer memory!

    (1) Plate reader quickly identifies GFP positive wells.
    (2) Only GFP positive wells are imaged, saving both time and computer memory.
    Variable bandwidth monochromator for sensitivity and specificity
    The bandwidths of the monochromators in the Cytation C10 fluorescence detection module is selectable from 9 nm to 50 nm, in 1 nm increments. Variable bandwidths allow you to optimize detection conditions for fluorophores that have unique excitation or emission parameters, for example, a narrow Stokes shift. Adjusting the bandwidth results in better performance than conventional monochromators.

    The plate reader optics of Cytation use a quad monochromator design with variable bandwidth. The bandwidth can be set anywhere between 9 and 50 nm in 1 nm increment. Large bandwidth settings (1) provide increased sensitivity and lower limits of detection. Small bandwidth settings (2) provide increased specificity when multiple signals are present, which reduces signal crosstalk and enhances assay performance
    Maintaining a consistent environment through CO2/O2 and temperature control
    Cytation C10 is well suited for live cell assays, with temperature control to 45 °C, plus linear and orbital shaking. A gas controller module is available to help regulate CO2 and O2 levels in the system for longer-term live cell requirements. A dual reagent injector provides quick inject/image capability for observing fast reactions.

    Successful live cell kinetic imaging relies on a consistent environment, including temperature control and CO2/O2 control and monitoring. Cytation C10 provides the perfect environment to grow and analyze live cells over time. Powerful movie maker and kinetic analysis software tools allow visualizing and analysis time-lapse experiments.
    Label-free cell counting
    It’s fast and convenient to count live cells using the available high contrast brightfield option. Higher contrast allows a direct and label-free method for object identification using Gen5 software to count cells and characterize cell proliferation in kinetic studies.


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