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BioTek 405 LS
Information
The 405™ LS Microplate Washer is the fifth generation Agilent-BioTek washer, known for superior performance and reliability for washing protocols ranging from gentle cell washing to vigorous ELISAs. The 405 LS has a simple user interface for creation and execution of wash, dispense, aspirate and maintenance protocols within the extensive onboard software. For use under computer control, BioTek’s LHC™ Software is available in standard and 21 CFR Part 11 compliant versions.
The optional BioStack™ is available for walk-away automation of up to 50 microplates.
• Magnetic and polystyrene bead assays, conventional ELISAs
• Patented Dual-Action™ manifold
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Configurations
Part # 384-well 96-well Optimized Cell Washing 4-Buffer Switching Ultrasonic Advantage Verify™ technology Biomagnetic Separation** Vacuum Filtration** 405LSHT• *• • • 405LSHTV• *• • • • 405LSU• • • • • 405LSUV• • • • • • 405LSUS• • • • • • 405LSUSQ• • • • • • • 405LSUVS• • • • • • • 405LSUVSQ• • • • • • • • 405LSR• • • 405LSRV• • • • 405LSRS• • • • 405LSRSQ• • • • • 405LSRVS• • • • • 405LSRVSQ• • • • • • * 96-well washing available with optional manifold PN 1170010, sold separately ** With available accessory module sold separately405™ LS Microplate Washer Configurations Feature Design HT Faster 384-well microplate washing 192-tube (16 x 12) manifold Dual-Action™ HT Includes Ultrasonic Advantage V Automated Internal 4-Buffer Switching S Ultrasonic Advantage™ Built-in ultrasonic cleaner for manifold self-maintenance U 96-/384-well microplate washing 96-tube (8 x 12) manifold Dual-Action™ R 96-well microplate washing 96-tube (8 x 12) manifold Q Verify™ technology Built-in clog detection for dispense and aspirate tubes -
specifications
GENERAL Microplate types 96- and 384-well Low profile and standard height Solid and filter bottom (option) Onboard software Create, edit or run multiple protocols Software (computer control) LHC2 pc software LHC2 Secure for 21 CFR Part 11 compliance (option) SiLA Compliant driver (option) Separation Biomagnetic separation, vacuum filtration (optional) Shaking Programmable up to 60 minutes Slow, medium, fast or variable Soaking Programmable up to 60 minutes Ultrasonic Advantage™ Yes (option) Verify™ clog detection Yes (option) Automation BioStack and 3rd party automation compatible BioSpa 8 Automated Incubator compatible WASHING Manifold types 96-well washing: 96-tube manifold – straight dispense tubes 96- and 384-well washing: 96-tube Dual-Action manifold – 20° angled dispense tubes 384-well washing (fast): 192-tube Dual-Action manifold – 7° angled dispense tubes Volume range 25 to 3,000 µL/well, in 1 µL increments Wash cycles 1 – 250 Buffer/reagent selection Auto switching (internal) for up to 4 buffers (option) Supply bottle 4 L or 10 L (optional) Dispense precision <3% CV: 300 µL/well (96-well washing) <4% CV: 80 µL/well (384-well washing) Residual volume <2 µL/well (96- & 384-well plates) 96-tube manifold for 96 wells; 192-tube for 384 wells Wash speed 96-wells, 300 µL/well, 3 cycles; <30 seconds 384-wells,100 µL/well, 3 cycles: <80 seconds 384-wells, 400 µL/well, 1 cycle: <20 seconds Flow rates High flow to low flow Optimized rates for cell assays Sterilization Chemical Vacuum filtration Selectable: Approximate final at 30 seconds: Lowest: -38 mmHg Low: -113 mmHg Medium: -200 mmHg High: -390 mmHg Highest: -506 mmHg Vacuum filtration time range: 5 to 999 seconds PHYSICAL CHARACTERISTICS Power 100 – 240 Volts AC. 50/60 Hz Dimensions 14″ W x 17″ D x 10″ H (35.6 x 43.2 x 25.4 cm) Weight With internal buffer switching – 36 lbs (16.5 kg) Without – 30 lbs (13.5 kg) REGULATORY Regulatory CE and TUV marked. ROHS compliant. In Vitro Diagnostic use models are available. -
Software
The 405 LS has extensive onboard software, accessed through the convenient touchscreen interface. Program all aspects of the system’s wash and maintenance parameters, quickly and easily and store them onboard for routine use.
LHC
LHC Software allows computer-based programming of multi-step processes and it is used for integrating BioTek’s multi-mode washer dispensers, washers and dispensers into robotic systems. You can operate the system under computer control via LHC, or create protocols in LHC and download to the instrument for recall and execution.LHC Secure software
LHC Secure software offers features to facilitate compliance to FDA directive 21 CFR Part 11
with computer control of BioTek’s Multi-Mode Washer Dispensers, Washers and Dispensers.LHC SiLA
LHC SiLA software driver enables BioTek washer and dispenser integration into SiLA compliant automated systems. Includes LHC software for computer control and third party integration of BioTek’s Multi-Mode Washer Dispensers, Washers and Dispensers. -
Features
Intuitive onboard User Interface or LHC™ pc software
The 405 LS has a simple user interface for creation and execution of wash, dispense, aspirate and maintenance protocols within the extensive onboard software. For use under computer control, BioTek’s LHC™ Software is available in standard and 21 CFR Part 11 compliant versions.Magnetic and polystyrene bead assays, conventional ELISAs
The 405 LS is ideal for many standard plate washing and dispensing protocols, but it also automates more complex operations like magnetic and polystyrene bead washing workflows – and it’s Luminex® xMap® approved. Bead washing is available for 96- and 384-well microplates.USB flash drive ports for convenient file transfer and storage
Two USB ports on the 405 TS enable convenient file transfer, storage and operation.Patented Dual-Action™ manifold
Cell-based assays are easily processed with the 405 Select LS, with its angled dispense tubes, extensive aspirate and dispense rates and plate position controls providing optimal conditions for washing even fragile cell layers.
Brochures
Application notes
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