The 405™ LS Microplate Washer is the fifth generation Agilent-BioTek washer, known for superior performance and reliability for washing protocols ranging from gentle cell washing to vigorous ELISAs. The 405 LS has a simple user interface for creation and execution of wash, dispense, aspirate and maintenance protocols within the extensive onboard software. For use under computer control, BioTek’s LHC™ Software is available in standard and 21 CFR Part 11 compliant versions.




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Part # |
384-well |
96-well |
Optimized Cell Washing |
4-Buffer Switching |
Ultrasonic Advantage |
Verify™ technology |
Biomagnetic Separation** |
Vacuum Filtration** |
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405LSHT |
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| 405™ LS Microplate Washer Configurations | ||
| Feature | Design | |
| HT | Faster 384-well microplate washing | 192-tube (16 x 12) manifold |
| Dual-Action™ | ||
| HT Includes Ultrasonic Advantage | ||
| V | Automated Internal 4-Buffer Switching | |
| S | Ultrasonic Advantage™ | Built-in ultrasonic cleaner for manifold self-maintenance |
| U | 96-/384-well microplate washing | 96-tube (8 x 12) manifold |
| Dual-Action™ | ||
| R | 96-well microplate washing | 96-tube (8 x 12) manifold |
| Q | Verify™ technology | Built-in clog detection for dispense and aspirate tubes |
| GENERAL | |
| Microplate types | 96- and 384-well |
| Low profile and standard height | |
| Solid and filter bottom (option) | |
| Onboard software | Create, edit or run multiple protocols |
| Software (computer control) | LHC2 pc software |
| LHC2 Secure for 21 CFR Part 11 compliance (option) | |
| SiLA Compliant driver (option) | |
| Separation | Biomagnetic separation, vacuum filtration (optional) |
| Shaking | Programmable up to 60 minutes |
| Slow, medium, fast or variable | |
| Soaking | Programmable up to 60 minutes |
| Ultrasonic Advantage™ | Yes (option) |
| Verify™ clog detection | Yes (option) |
| Automation | BioStack and 3rd party automation compatible |
| BioSpa 8 Automated Incubator compatible | |
| WASHING | |
| Manifold types | 96-well washing: 96-tube manifold – straight dispense tubes |
| 96- and 384-well washing: 96-tube Dual-Action manifold – 20° angled dispense tubes | |
| 384-well washing (fast): 192-tube Dual-Action manifold – 7° angled dispense tubes | |
| Volume range | 25 to 3,000 µL/well, in 1 µL increments |
| Wash cycles | 1 – 250 |
| Buffer/reagent selection | Auto switching (internal) for up to 4 buffers (option) |
| Supply bottle | 4 L or 10 L (optional) |
| Dispense precision | <3% CV: 300 µL/well (96-well washing) |
| <4% CV: 80 µL/well (384-well washing) | |
| Residual volume | <2 µL/well (96- & 384-well plates) |
| 96-tube manifold for 96 wells; 192-tube for 384 wells | |
| Wash speed | 96-wells, 300 µL/well, 3 cycles; <30 seconds |
| 384-wells,100 µL/well, 3 cycles: <80 seconds | |
| 384-wells, 400 µL/well, 1 cycle: <20 seconds | |
| Flow rates | High flow to low flow |
| Optimized rates for cell assays | |
| Sterilization | Chemical |
| Vacuum filtration | Selectable: Approximate final at 30 seconds: |
| Lowest: -38 mmHg | |
| Low: -113 mmHg | |
| Medium: -200 mmHg | |
| High: -390 mmHg | |
| Highest: -506 mmHg | |
| Vacuum filtration time range: 5 to 999 seconds | |
| PHYSICAL CHARACTERISTICS | |
| Power | 100 – 240 Volts AC. 50/60 Hz |
| Dimensions | 14″ W x 17″ D x 10″ H |
| (35.6 x 43.2 x 25.4 cm) | |
| Weight | With internal buffer switching – 36 lbs (16.5 kg) |
| Without – 30 lbs (13.5 kg) | |
| REGULATORY | |
| Regulatory | CE and TUV marked. ROHS compliant. In Vitro Diagnostic use models are available. |
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