The ELISA workflow

The ELISA Workflow using (automated) solutions of Agilent BioTek

The enzyme-linked immunosorbent assay, also known as ELISA is a widely used technique for the detection of, for example, an antigen in a sample. An antigen is a molecule located on the surface of a pathogen. With this plate-based technique you can detect and quantify soluble substances such as proteins. ELISAs are used in a wide range of research areas and can be analyzed within a broad variety of sample types such as, blood (serum and plasma), tissue, cell lysates, homogenates, and also food samples.

The illustration below shows the steps required for a common ELISA assay.

At BioSPX, we offer many solutions that complete the ELISA workflow through high-quality equipment with one goal: An optimized workflow.
Our microplate washer is designed to control the procedure of washing experimental samples arranged in plate-based formats and can be used to wash away background signals related to unbound, conjugated antibodies. Moreover, the microplate readers are suitable for performing UV-Vis absorbance measurements. This comes with Gen5 software for additional powerful data analysis.

Working with high-throughput?

The microplate readers are automation compatible to create walkaway processing. To automate reading processes for multiple plates, a robot-friend configuration of the plate readers interfaces with BioStack Microplate Stacker. Process 10, 30 or 50 microplates automatically and efficiently, freeing your time for other tasks. The data measurements can also be automated, substraction with reference wavelenght, cut off value for direct visualization of your data. All of these data can have an automated export to Excel and PDF for example.

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