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BioTek MultiFlo FX
Information
MultiFlo™ FX is an automated reagent dispenser for 6- to 1536-well microplates. Its unique Parallel Dispense™ technology allows up to four independent reagents to be dispensed in parallel without potential carryover. What makes MultiFlo FX “multi-mode” is its configurability: you can add a wash module or the RAD™ module, for random access individual well dispensing and bulk dispensing larger wells. Everything you need for fast, intuitive programming and operation is available through the color touchscreen. MultiFlo FX configured with either the wash module or RAD technology replaces up to five liquid handlers in a single compact platform.
- Multi-mode dispensing can replace up to 4 dispensers and a washer
- Parallel Dispense™ technology: peristaltic and/or syringe pumps
- RAD™ technology for dispensing to individual wells
- Cost savings through low prime volume and single tube access
- Cell friendly angled dispense and/or wash tubes won’t disrupt cell layers
- Walkaway automation with BioStack 4, for lidded or unlidded plates
Configurations
ConfigurationsMFX MFXP* MFXW** MFXPW** MFXR*** MFXPR*** Unconfigured base instrument • 1 peripump dispenser only • Wash module only • 1 peripump dispenser and wash module • RAD technology with one peripump • RAD technology with two peripumps • *P configurations: include one plastic tip cassette
**W configurations: include 96/384 wash manifold
***R configurations: include one plastic tip multi-channel or RAD cassette.Dispenser Modules (purchased separately)
7210010 – External Peristaltic Pump Dispenser Module
Adds 1 peristaltic pump dispenser to MultiFlo FX base instruments. For 6- to 1536-well microplates. Includes 1 plastic tip cassette.
Note: 7210010 is not required and is not compatible with MFXR or MFXPR7210008 – External Dual Syringe Dispenser Module, Autoclavable
Adds 2 syringe drive dispensers to MultiFlo FX base instruments.7210009 – External Dual Syringe Dispenser Module, Non-Autoclavable
Adds 2 syringe drive dispensers to MultiFlo FX base instruments.Specifications
GENERAL Microplate types 96-, 384- and 1536-well Low profile and standard height Solid and filter bottom (option) Onboard software Create, edit or run multiple protocols Software (computer control) LHC2 Software LHC2 Secure for 21 CFR Part 11 compliance (option) SiLA Compliant driver (option) Separation Biomagnetic separation, vacuum filtration (optional) Shaking Programmable up to 60 minutes Slow, medium, fast or variable Soaking Programmable up to 60 minutes Ultrasonic Advantage™ Standard on most configurations Automation BioStack and 3rd party automation compatible BioSpa 8 Automated Incubator compatible WASHING Manifold types 96-well washing: 96-tube manifold – straight dispense tubes 96- and 384-well washing: 96-tube Dual-Action manifold – 20° angled dispense tubes 384-well washing (fast): 192-tube Dual-Action manifold – 7° angled dispense tubes 1536-well washing: Two 32-tube dispense manifolds – 7° angled dispense tubes Volume range 3 – 3,000 µL/well in 1 µL increments Wash cycles 1 – 250 Buffer/reagent selection Auto switching module for up to 4 buffers (option) Supply bottle 4 L or 10 L (optional) Dispense accuracy 3% Dispense precision <3% CV (model dependent) Residual volume <2 µL/well Wash speed 96 wells, 300 µL/well, 96-tube manifold: 13 seconds 384 wells, 100 µL/well, 192-tube manifold: 17 seconds 1536 wells, 10 µL/wells, two 32-tube manifolds: 36 seconds Flow rates High flow to low flow Optimized rates for cell assays Sterilization Chemical Vacuum range for filtration Selectable: 0 to -380 mmHg (final at 30s) Filtration time: 5 – 999 seconds DISPENSING – PERISTALTIC PUMP (MULTI-CHANNEL) Manifold types 8-tip (1 x 8) cassette with plastic, 316 SS tubes or sapphire jeweled 316 SS tubes Fluid delivery 1 or 2 peristaltic pumps Dispense speed 96 wells, 10 µL /well: 8 seconds 384 wells, 5 µL /well: 12 seconds 1536 wells, 1 µL /well: 27 seconds Volume range 500 nL – 3,000 µL/well, selectable in 1 µL increments Flow rates User programmable rates from high to low Optimized rates for cell assays Dispense performance 1 µL cassette: Recommended Volume Range: 1 – 50 µL Dispense Accuracy: +5% at 1 µL Dispense Precision: <5% CV at 1 µL <10% CV at 500 nL Minimum Prime Volume: 1.20 mL 5 µL cassette: Recommended Volume Range: 5 – 2,500 µL Dispense Accuracy: +2.0% at 5 µL Dispense Precision: <2.5% CV at 5 µL Minimum Prime Volume: 4.23 mL 10 µL cassette: Recommended Volume Range: 10 – 3,000 µL Dispense Accuracy: +2.0% at 10 µL Dispense Precision: <2.0% CV at 10 µL Minimum Prime Volume: 7.36 mL Recommended cassette replacement interval 1 µL Cassette: 1,000 384-well microplates at 5 µL well 5 µL Cassette: 1,000 96-well microplates at 50 µL well 10 µL Cassette: 1,000 96-well microplates at 100 µL well Sterilization Autoclave, chemical DISPENSING – SYRINGE PUMP Manifold types 96-well dispensing: One 16-tube (2 x 8) manifold – 316 SS tubes 96-/384-well dispensing: Two 16-tube (1 x 16) manifolds – 316 SS tubes 1536-well dispensing: Two 32-tube (1 x 32) manifolds – sapphire jeweled 316 SS or 316 stainless steel tubes Fluid delivery Two positive displacement syringe drives Dispense speed 20 µL /well, 96 wells, 1 x 16 tubes: 5.25 seconds 20 µL /well, 384 wells, 1 x 16 tubes: 14 seconds 3 µL /well 1536 wells, 2 x 32 tubes: 7 seconds Volume range 3 – 3,000 µL/well, selectable in 1 µL increments Minimum Prime Volume: 12 mL Flow rates User programmable rates from high to low Dispense accuracy +1 µL at 5 µL +1 µL at 20 µL +1% at 100 µL Dispense precision <5% CV at 5 µL <2.5% CV at 20 µL <1% CV at 100 µL Supply bottle 1 L or 2 L Sterilization Chemical, autoclavable option PHYSICAL CHARACTERISTICS Power 100 – 240 Volts AC. 50/60 Hz Dimensions 16.5″ W x 18″ D x 12.5″ H (42 x 46 x 32 cm) Weight 32 lbs (14.5 kg) REGULATORY Regulatory CE and TUV marked. ROHS compliant. In Vitro Diagnostic use models are available. Software
The MultiFlo FX extensive onboard software, accessed through the convenient touchscreen interface. With an intuitive menu-driven interface. Program all aspects of the system’s dispense, wash and maintenance parameters, quickly and easily.
LHC
LHC Software allows computer-based programming of multi-step processes and it is used for integrating BioTek’s multi-mode washer dispensers, washers and dispensers into robotic systems. You can operate the system under computer control via LHC, or create protocols in LHC and download to the instrument for recall and execution.LHC Secure software
LHC Secure software offers features to facilitate compliance to FDA directive 21 CFR Part 11
with computer control of BioTek’s Multi-Mode Washer Dispensers, Washers and Dispensers.LHC SiLA software
LHC SiLA softwaredriver enables BioTek washer and dispenser integration into SiLA compliant automated systems. Includes LHC software for computer control and third party integration of BioTek’s Multi-Mode Washer Dispensers, Washers and Dispensers.AMX Automated Media Exchange
Spheroidal 3D cell culture is increasingly common for many research areas as it better mimics the in vivo environment. It can be challenging to perform media exchanges and washes without accidental cell structure loss.
BioTek’s patent-pending AMX™ module for the MultiFlo™ FX enables gentle automated media exchange to protect and encourage proliferation of spheroids, tumoroids and other 3D cell structures, 2D and suspension cells in microplate-based assays The AMX module uses both peristaltic pumps, one to dispense and one to aspirate media from the plate wells.
The software operates the pumps slowly and gently so cells and cell structures aren’t disturbed during the media exchange, and the x-y-z positions for the aspirate and dispense tubes are adjustable to avoid contact, etc with cell structures in the wells.
The two peristaltic pumps and software control the media flow through the cassette tubing
and manifolds into the microplate. The arrows indicate the flow pattern.Close-up views show the critical positioning of the dispense tubes (left) and aspirate tubes (right) during media exchanges. AMX Benefits:- Automates media exchange for spheroids, tumoroids and other 3D cell structures, 2D and other cell structures
- Helps protect cells and encourage cell structure growth
- AMX module can be added to MultiFlo FX and easily exchanged with other modules for workflow versatility
Features
Multi-mode dispensing can replace up to four dispensers and a washer
Providing unsurpassed liquid handling flexibility, MultiFlo FX’s modular base can accommodate 1 or 2 peristaltic pumps, a 2-syringe pump module and a wash module or a single channel dispense. MultiFlo FX takes the place of multiple instruments, but is compact and very simple to use.Parallel Dispense™ technology: peristaltic and/or syringe pumps
Peristaltic pumps and syringe pumps each offer benefits; peri-pumps help save precious reagent, while syringe pumps are fast and precise. MultiFlo FX can be configured with 2 peri and 2 syringe pumps, allowing up to four reagents to be dispensed in parallel, without carryover.RAD™ technology for dispensing to individual wells
When the liquid handling workflow calls for individual well dispensing along with rapid bulk dispensing, MultiFlo FX can handle it. The unique RAD module provides single channel dispensing to discrete wells of a 48-, 96- or 384-well plate. For rapid single channel dispensing to 6- to 24-well plate, the 8-to-1 RAD cassette is perfect for the task.Cost savings through low prime volume and single tube access
With programmable prime volumes, backflush capability and the unique single tube access with RAD, you’ll save on costly reagents for all your dispensing processes.Cell friendly angled dispense and/or wash tubes won’t disrupt cell layers
For sensitive cell-based assays, the dispense tips on the MultiFlo FX dispense manifolds and wash manifolds are angled, allowing gentle reagent dispense down the well sides. This helps keep cell layers intact, even through multiple-step operations.Walkaway automation with BioStack 4 or BioSpa 8 for lidded or unlidded plates
Integration BioStack to MultiFlo FX allows automated processing of multiple plates at a time, without manual intervention. MultiFlo FX integrated to BioSpa 8 adds automated media exchange and reagent addition to automated live cell workflows.
Brochures
Application notes
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