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Agilent

BioTek MultiFlo FX

Short product description
Automated multimode reagent dispenser for 6- to 1536-well microplates

Information

MultiFlo™ FX is an automated reagent dispenser for 6- to 1536-well microplates. Its unique Parallel Dispense™ technology allows up to four independent reagents to be dispensed in parallel without potential carryover. What makes MultiFlo FX “multi-mode” is its configurability: you can add a wash module or the RAD™ module, for random access individual well dispensing and bulk dispensing larger wells. Everything you need for fast, intuitive programming and operation is available through the color touchscreen. MultiFlo FX configured with either the wash module or RAD technology replaces up to five liquid handlers in a single compact platform.

  • Multi-mode dispensing can replace up to 4 dispensers and a washer
  • Parallel Dispense™ technology: peristaltic and/or syringe pumps
  • RAD™ technology for dispensing to individual wells
  • Cost savings through low prime volume and single tube access
  • Cell friendly angled dispense and/or wash tubes won’t disrupt cell layers
  • Walkaway automation with BioStack 4, for lidded or unlidded plates
  • Configurations

    MFX
    MFXP*
    MFXW**
    MFXPW**
    MFXR***
    MFXPR***
    Unconfigured base instrument
    1 peripump dispenser only
    Wash module only
    1 peripump dispenser and wash module
    RAD technology with one peripump
    RAD technology with two peripumps
    *P configurations: include one plastic tip cassette
    **W configurations: include 96/384 wash manifold
    ***R configurations: include one plastic tip multi-channel or RAD cassette.Dispenser Modules (purchased separately)
    7210010 – External Peristaltic Pump Dispenser Module
    Adds 1 peristaltic pump dispenser to MultiFlo FX base instruments. For 6- to 1536-well microplates. Includes 1 plastic tip cassette.
    Note: 7210010 is not required and is not compatible with MFXR or MFXPR7210008 – External Dual Syringe Dispenser Module, Autoclavable
    Adds 2 syringe drive dispensers to MultiFlo FX base instruments.7210009 – External Dual Syringe Dispenser Module, Non-Autoclavable
    Adds 2 syringe drive dispensers to MultiFlo FX base instruments.
  • Specifications

    GENERAL
    Microplate types 96-, 384- and 1536-well
    Low profile and standard height
    Solid and filter bottom (option)
    Onboard software Create, edit or run multiple protocols
    Software (computer control) LHC2 Software
    LHC2 Secure for 21 CFR Part 11 compliance (option)
    SiLA Compliant driver (option)
    Separation Biomagnetic separation, vacuum filtration (optional)
    Shaking Programmable up to 60 minutes
    Slow, medium, fast or variable
    Soaking Programmable up to 60 minutes
    Ultrasonic Advantage™ Standard on most configurations
    Automation BioStack and 3rd party automation compatible
    BioSpa 8 Automated Incubator compatible
    WASHING
    Manifold types 96-well washing: 96-tube manifold – straight dispense tubes
    96- and 384-well washing: 96-tube Dual-Action manifold – 20° angled dispense tubes
    384-well washing (fast): 192-tube Dual-Action manifold – 7° angled dispense tubes
    1536-well washing: Two 32-tube dispense manifolds – 7° angled dispense tubes
    Volume range 3 – 3,000 µL/well in 1 µL increments
    Wash cycles 1 – 250
    Buffer/reagent selection Auto switching module for up to 4 buffers (option)
    Supply bottle 4 L or 10 L (optional)
    Dispense accuracy 3%
    Dispense precision <3% CV (model dependent)
    Residual volume <2 µL/well
    Wash speed 96 wells, 300 µL/well, 96-tube manifold: 13 seconds
    384 wells, 100 µL/well, 192-tube manifold: 17 seconds
    1536 wells, 10 µL/wells, two 32-tube manifolds: 36 seconds
    Flow rates High flow to low flow
    Optimized rates for cell assays
    Sterilization Chemical
    Vacuum range for filtration Selectable: 0 to -380 mmHg (final at 30s)
    Filtration time: 5 – 999 seconds
    DISPENSING – PERISTALTIC PUMP (MULTI-CHANNEL)
    Manifold types 8-tip (1 x 8) cassette with plastic, 316 SS tubes or sapphire jeweled 316 SS tubes
    Fluid delivery 1 or 2 peristaltic pumps
    Dispense speed 96 wells, 10 µL /well: 8 seconds
    384 wells, 5 µL /well: 12 seconds
    1536 wells, 1 µL /well: 27 seconds
    Volume range 500 nL – 3,000 µL/well, selectable in 1 µL increments
    Flow rates User programmable rates from high to low
    Optimized rates for cell assays
    Dispense performance 1 µL cassette:
    Recommended Volume Range: 1 – 50 µL
    Dispense Accuracy: +5% at 1 µL
    Dispense Precision: <5% CV at 1 µL
    <10% CV at 500 nL
    Minimum Prime Volume: 1.20 mL
    5 µL cassette:
    Recommended Volume Range: 5 – 2,500 µL
    Dispense Accuracy: +2.0% at 5 µL
    Dispense Precision: <2.5% CV at 5 µL
    Minimum Prime Volume: 4.23 mL
    10 µL cassette:
    Recommended Volume Range: 10 – 3,000 µL
    Dispense Accuracy: +2.0% at 10 µL
    Dispense Precision: <2.0% CV at 10 µL
    Minimum Prime Volume: 7.36 mL
    Recommended cassette replacement interval 1 µL Cassette: 1,000 384-well microplates at 5 µL well
    5 µL Cassette: 1,000 96-well microplates at 50 µL well
    10 µL Cassette: 1,000 96-well microplates at 100 µL well
    Sterilization Autoclave, chemical
    DISPENSING – SYRINGE PUMP
    Manifold types 96-well dispensing:
    One 16-tube (2 x 8) manifold – 316 SS tubes
    96-/384-well dispensing:
    Two 16-tube (1 x 16) manifolds – 316 SS tubes
    1536-well dispensing:
    Two 32-tube (1 x 32) manifolds – sapphire jeweled 316 SS or 316 stainless steel tubes
    Fluid delivery Two positive displacement syringe drives
    Dispense speed 20 µL /well, 96 wells, 1 x 16 tubes: 5.25 seconds
    20 µL /well, 384 wells, 1 x 16 tubes: 14 seconds
    3 µL /well 1536 wells, 2 x 32 tubes: 7 seconds
    Volume range 3 – 3,000 µL/well, selectable in 1 µL increments
    Minimum Prime Volume: 12 mL
    Flow rates User programmable rates from high to low
    Dispense accuracy +1 µL at 5 µL
    +1 µL at 20 µL
    +1% at 100 µL
    Dispense precision <5% CV at 5 µL
    <2.5% CV at 20 µL
    <1% CV at 100 µL
    Supply bottle 1 L or 2 L
    Sterilization Chemical, autoclavable option
    PHYSICAL CHARACTERISTICS
    Power 100 – 240 Volts AC. 50/60 Hz
    Dimensions 16.5″ W x 18″ D x 12.5″ H (42 x 46 x 32 cm)
    Weight 32 lbs (14.5 kg)
    REGULATORY
    Regulatory CE and TUV marked. ROHS compliant. In Vitro Diagnostic use models are available.
  • Software

    The MultiFlo FX extensive onboard software, accessed through the convenient touchscreen interface. With an intuitive menu-driven interface. Program all aspects of the system’s dispense, wash and maintenance parameters, quickly and easily.

    LHC
    LHC Software allows computer-based programming of multi-step processes and it is used for integrating BioTek’s multi-mode washer dispensers, washers and dispensers into robotic systems. You can operate the system under computer control via LHC, or create protocols in LHC and download to the instrument for recall and execution.

    LHC Secure software
    LHC Secure software offers features to facilitate compliance to FDA directive 21 CFR Part 11
    with computer control of BioTek’s Multi-Mode Washer Dispensers, Washers and Dispensers.

    LHC SiLA software
    LHC SiLA softwaredriver enables BioTek washer and dispenser integration into SiLA compliant automated systems. Includes LHC software for computer control and third party integration of BioTek’s Multi-Mode Washer Dispensers, Washers and Dispensers.

  • AMX Automated Media Exchange

    Spheroidal 3D cell culture is increasingly common for many research areas as it better mimics the in vivo environment. It can be challenging to perform media exchanges and washes without accidental cell structure loss.

    BioTek’s patent-pending AMX™ module for the MultiFlo™ FX enables gentle automated media exchange to protect and encourage proliferation of spheroids, tumoroids and other 3D cell structures, 2D and suspension cells in microplate-based assays The AMX module uses both peristaltic pumps, one to dispense and one to aspirate media from the plate wells.

    The software operates the pumps slowly and gently so cells and cell structures aren’t disturbed during the media exchange, and the x-y-z positions for the aspirate and dispense tubes are adjustable to avoid contact, etc with cell structures in the wells.

     

    The two peristaltic pumps and software control the media flow through the cassette tubing
    and manifolds into the microplate. The arrows indicate the flow pattern.
     

    Close-up views show the critical positioning of the dispense tubes (left) and aspirate tubes (right) during media exchanges.
    AMX Benefits:

    • Automates media exchange for spheroids, tumoroids and other 3D cell structures, 2D and other cell structures
    • Helps protect cells and encourage cell structure growth
    • AMX module can be added to MultiFlo FX and easily exchanged with other modules for workflow versatility
  • Features

    Multi-mode dispensing can replace up to four dispensers and a washer
    Providing unsurpassed liquid handling flexibility, MultiFlo FX’s modular base can accommodate 1 or 2 peristaltic pumps, a 2-syringe pump module and a wash module or a single channel dispense. MultiFlo FX takes the place of multiple instruments, but is compact and very simple to use.

    Parallel Dispense™ technology: peristaltic and/or syringe pumps
    Peristaltic pumps and syringe pumps each offer benefits; peri-pumps help save precious reagent, while syringe pumps are fast and precise. MultiFlo FX can be configured with 2 peri and 2 syringe pumps, allowing up to four reagents to be dispensed in parallel, without carryover.

    RAD™ technology for dispensing to individual wells
    When the liquid handling workflow calls for individual well dispensing along with rapid bulk dispensing, MultiFlo FX can handle it. The unique RAD module provides single channel dispensing to discrete wells of a 48-, 96- or 384-well plate. For rapid single channel dispensing to 6- to 24-well plate, the 8-to-1 RAD cassette is perfect for the task.

    Cost savings through low prime volume and single tube access
    With programmable prime volumes, backflush capability and the unique single tube access with RAD, you’ll save on costly reagents for all your dispensing processes.

    Cell friendly angled dispense and/or wash tubes won’t disrupt cell layers
    For sensitive cell-based assays, the dispense tips on the MultiFlo FX dispense manifolds and wash manifolds are angled, allowing gentle reagent dispense down the well sides. This helps keep cell layers intact, even through multiple-step operations.

    Walkaway automation with BioStack 4 or BioSpa 8 for lidded or unlidded plates
    Integration BioStack to MultiFlo FX allows automated processing of multiple plates at a time, without manual intervention. MultiFlo FX integrated to BioSpa 8 adds automated media exchange and reagent addition to automated live cell workflows.

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