BioTek Synergy Neo2

Agilent

BioTek Synergy Neo2

Short product description

Hybrid Multi-Mode Reader for demanding applications

Information

Synergy™ Neo2 Multi-Mode Microplate Reader delivers the speed and exceptional performance required for demanding applications. Its patented Hybrid Technology™ , with filter- and monochromator- based optics including multiple PMTs, ensures ultrafast, high performance in all detection modes without compromise.

Agilent BioTek’s fastest, highest performing multi-mode microplate reader
Scientific quad monochromators with continuously variable bandwidth from 3 nm to 50 nm
Live cell assay optimized: CO2/O2 control, incubation to 65 °C and direct bottom illumination
Multiple PMTs including dual top PMTs for ultra-fast ratiometric assays
Gen6 Data Analysis Software; Gen6 Secure for 21 CFR Part 11 compliance

Specifications

GENERAL
Detection modes	UV-Vis absorbance
	Fluorescence intensity
	Luminescence
	Fluorescence polarization
	Time-resolved fluorescence
	Alpha
Read methods	Endpoint, kinetic, spectral scanning, well area scanning
Microplate types	6- to 1536-well plates
Other labware supported	Petri and cell culture dishes
Other labware supported	Take3 Micro-Volume Plates
Temperature control	4-Zone incubation to 65 °C with Condensation Control™
Temperature control	+0.2 °C at 37 °C
Shaking	Linear, orbital, double orbital
Software	Gen6™ Microplate Reader and Imager Software
Software	Gen5 Secure for 21 CFR Part 11 compliance (option)
Automation	BioStack and 3rd party automation compatible
Automation	BioSpa 8 Automated Incubator compatible
CO2 and O2 control (option)	Range: 0 – 20% (CO2); 1 – 19% (O2)
	Control Resolution: + 0.1% (CO2 and O2)
	Stability: +0.2% at 5% CO2; +0.2%at 1% O2
	Models for CO2 /O2 or CO2 only are available
Barcode reader	1D and 2D camera-based scanner
ABSORBANCE
Light source	Xenon flash
Detector	photodiode
Wavelength selection	monochromator
Wavelength range	230 – 999 nm, 1 nm increments
Monochromator bandwidth	2 nm (230-285 nm), 4 nm (>285 nm)
Dynamic range	0 – 4.0 OD
Resolution	0.0001 OD
Pathlength correction	yes
Monochromator wavelength accuracy	+2 nm
Monochromator wavelength repeatability	+0.2 nm
OD accuracy	<1% at 2.0 OD
OD accuracy	<3% at 3.0 OD
OD linearity	<1% from 0 to 3.0 OD
OD repeatability	<0.5% at 2.0 OD
Stray light	0.03% at 230 nm
Reading speed (kinetic)	96 well: 6 seconds
	384 well: 11 seconds
	1536 well: 25 seconds
FLUORESCENCE INTENSITY
Light source	Xenon flash
Detector	Dual top PMTs
	Single top PMT (option)
	Low noise PMT (bottom filter system)
	Red shifted PMT (top/bottom monochromator system)
Wavelength selection	Quad monochromators (top/bottom)
Wavelength selection	Filters (top/bottom)
Wavelength range	Monochromators: 250 – 850 nm
Wavelength range	Filters (dual PMT): 200 – 850 nm
Monochromator bandwidth	Variable, from 3 to 50 nm in 1 nm increments
Dynamic range	7 decades
Sensitivity	Filters:
	Fluorescein 0.2 pM (4 amol/well, 20 µL, 384-well low volume plate) – top
	Fluorescein 1 pM (10 amol/well, 10 µL, 1536-well plate) – top
	Fluorescein 1 pM (0.1 fmol/well, 100 µL, 384-well plate) – bottom

	Quad monochromator:
	Fluorescein 2 pM (40 amol/well, 20 µL, 384-well low volume plate) – top
	Fluorescein 2.5 pM (0.25 fmol/well, 100 µL, 384-well plate) – bottom
Reading speed (kinetic)	96 well: 6 seconds
	384 well: 11 seconds
	1536 well: 25 seconds
LUMINESCENCE
Wavelength range	300 – 700 nm
Dynamic range	> 6 decades
Sensitivity	5 amol ATP typical (384-well low volume plate)
FLUORESCENCE POLARIZATION
Light source	Xenon flash
Detector	Dual PMT or single PMT (option)
Wavelength selection	Filters
Wavelength range	280 – 850 nm
Sensitivity	1 mP standard deviation at 1 nM fluorescein
	(384-well low volume plate)
	1.5 mP standard deviation at 1 nM fluorescein (1536-well plate)
TIME-RESOLVED FLUORESCENCE
Light source	Xenon flash
Detector	Dual PMT or single PMT (option)
Wavelength selection	Quad monochromators (top/bottom)
Wavelength selection	Filters (top/bottom)
Wavelength range	Monos: 250 – 850 nm
Wavelength range	Filters (dual PMT): 200 – 850 nm
Sensitivity	Europium 40 fM (384-well low volume plate)
Sensitivity	Europium 70 fM (1536-well plate)
ALPHA DETECTION
Light source	100 mW 680 nm laser
Detector	PMT
Wavelength selection	Filters (top)
Sensitivity	100 amol LCK peptide (384-well low volume plate)
Read speed	96 well: 30 seconds
	384 well: 1 minute 50 seconds
	1536 well: 7 minutes 20 seconds
REAGENT DISPENSERS
Supported detection modes	All modes
Number	2 syringe pumps
Supported labware	6- to 384-well plates, Petri dishes
Dead volume	1.1 mL with back flush
Dispense volume	5 – 1000 µL in 1 µL increments
Dispense accuracy	+ 1 µL or 2%
Dispense precision	< 2% at 50-200 µL
PHYSICAL CHARACTERISTICS
Power	250 Watts max.
Dimensions	15.4″ W x 20.7″ D x 16.1″ H
Dimensions	(39 x 52.5 x 41 cm)
Weight	78 lbs (35 kg)
REGULATORY
Regulatory	CE and TUV marked. RoHS Compliant. Models for In Vitro Diagnostic use are available.

Software

Gen6 software controls all BioTek automated microplate readers, configuring system and data collection parameters, analysis and a variety of reporting and exporting features. One-click export to Excel® makes it fast and easy to export displayed data.
Gen5 Secure software is available for GxP compliant laboratories with features that support 21 CFR Part 11 rules.
Micro Volume Plates
Micro-volume quantification is fast and easy with the Take3™ and Take3 Trio Micro-Volume plates, used in your BioTek microplate reader. Measure multiple 2 µL samples at a time, without diluting and without needing specialized equipment. Pre-programmed nucleic acid and protein protocols in Gen6 make quantification fast and easy.
- Low volume 2 µL nucleic acid and protein quantification
- Pre-programmed protocols in Gen6 Software
- Absorbance and fluorescence detection
- Easy, wipe-off maintenance
- Read 2 µL microspots, BioCells or standard cuvettes
Robotics

BioTek’s robotic instruments can help automate workflows in your laboratory. Many life science workflows benefit from expanded automation, for increased throughput or process efficiency. BioTek offers unique automation solutions that integrate with our microplate washers, dispensers, readers and imagers. BioStack is a compact stacker that offers fast plate exchange options for 50 plates or more, and patented plate de-lidding and re-lidding with BioStack 4 . BioSpa 8 Automated Incubator links BioTek readers or imagers together with washers and dispensers for full workflow automation of up to 8 microplates. Temperature, CO2/O2 and humidity levels are controlled and monitored through the BioSpa software to maintain an ideal environment for cell cultures during all experimental stages.
Features

BioTek’s fastest, highest performing multi-mode microplate reader.
With kinetic reading speeds as fast as 6 seconds, Synergy Neo2 is a workhorse in the HTS lab. Integrating the compact BioStack enables rapid, automated multiple plate processing for increased efficiency and throughput.

Scientific quad monochromators with continuously variable bandwidth from 3 nm to 50 nm
Synergy Neo2 has quadruple scientific monochromators, with the fluorescence module offering variable bandwidths from 3 nm to 50 nm, selectable in 1 nm increments. Variable bandwidths allow you to optimize detection conditions for fluorophores that have unique excitation or emission parameters, for example, a narrow Stokes shift, to provide better performance than conventional monochromators.

Live cell assay optimized: CO2/O2 control, incubation to 65 °C and direct bottom illumination
Temperature control to 65 °C, plus linear and orbital shaking and an available gas controller module optimize the environment in Synergy Neo2 for live cell assays. The dedicted filter-based bottom optical path directly illuminates the plate bottom, increasing the sensitivity of live cell measurements, while a dual reagent injector provides quick inject/image capability for observing fast reactions.

Multiple PMTs including dual top PMTs for ultra-fast ratiometric assays
Up to 4 PMTs are available in the highly configurable, modular Synergy Neo2, for ultra-fast measurements in all detection modes. Two top PMTs meet the requirements for ultrafast ratiometric assays.

Gen6 Data Analysis Software; Gen5 Secure for 21 CFR Part 11 compliance
Gen6 Microplate Reader and Imager Software doesn’t compromise on usability or data analysis performance – protocol creation is intuitive and flexible, and the data analysis capability rivals standalone packages. For the GxP lab, Gen5 Secure software offers features to help comply with 21 CFR Part 11, including electronic signature, data security and multiple level permissions.

Winner of the SelectScience® Reviewers’ Choice Award for Drug Discovery & Development Product of the Year 2017
Synergy Neo2 consistently rates high among users – noted by the SelectScience Reviewers’ Choice Award for Drug Discovery & Development Product of the Year 2017 and Scientists’ Choice Awards® Best New Life Sciences Product of 2015.