The Agilent BioTek Synergy Neo2 multimode microplate reader features patented Hybrid Technology, with independent optical paths that ensure excellent performance in all detection modes. Variable bandwidth quad monochromators, sensitive high transmission filter-based optics, laser TRF, and up to 4 PMTs provide ultrafast measurements.
This HTS multimode reader features advanced environmental controls, including CO2/O2 control, incubation to 70 °C, and variable shaking support live cell assays. The compatible BioStack delivers walk-away automation, high throughput, barcode-labeled filter cubes to streamline workflows and limit errors. Powerful Gen5 software provides complete reader control and powerful data analysis.
Features:
Dual PMT = Fast Reading SpeedFor assays that require ratiometric measurements, such as fluorescence polarization, FRET and TR-FRET, a dual PMT system provides blazing fast throughput. Two emission signals are captured simultaneously, effectively doubling the measurement speed.
Two lasers: For TRF, TR-FRET and AlphaIn addition to the standard Xenon lamp in Synergy Neo2, a dedicated laser-based optical systems provide increased sensitivity and fast measurements for TRF and Alpha assays helping to generate larger assay windows and better assay z-primes.
Synergy Neo2 is well suited for many applications requiring a variety of detection modes, from UV-Vis absorbance to complex TR-FRET and FP measurements. Visit the technology page to learn more about all the detection modes available with Synergy Neo2.
| GENERAL | |
| Detection modes | UV-Vis absorbance |
| Fluorescence intensity | |
| Luminescence | |
| Fluorescence polarization | |
| Time-resolved fluorescence | |
| Alpha | |
| Read methods | Endpoint, kinetic, spectral scanning, well area scanning |
| Microplate types | 6- to 1536-well plates |
| Other labware supported | Petri and cell culture dishes |
| Take3 Micro-Volume Plates | |
| Temperature control | 4-Zone incubation to 65 °C with Condensation Control™ |
| +0.2 °C at 37 °C | |
| Shaking | Linear, orbital, double orbital |
| Software | Gen6™ Microplate Reader and Imager Software |
| Gen5 Secure for 21 CFR Part 11 compliance (option) | |
| Automation | BioStack and 3rd party automation compatible |
| BioSpa 8 Automated Incubator compatible | |
| CO2 and O2 control (option) | Range: 0 – 20% (CO2); 1 – 19% (O2) |
| Control Resolution: + 0.1% (CO2 and O2) | |
| Stability: +0.2% at 5% CO2; +0.2%at 1% O2 | |
| Models for CO2 /O2 or CO2 only are available | |
| Barcode reader | 1D and 2D camera-based scanner |
| ABSORBANCE | |
| Light source | Xenon flash |
| Detector | photodiode |
| Wavelength selection | monochromator |
| Wavelength range | 230 – 999 nm, 1 nm increments |
| Monochromator bandwidth | 2 nm (230-285 nm), 4 nm (>285 nm) |
| Dynamic range | 0 – 4.0 OD |
| Resolution | 0.0001 OD |
| Pathlength correction | yes |
| Monochromator wavelength accuracy | +2 nm |
| Monochromator wavelength repeatability | +0.2 nm |
| OD accuracy | <1% at 2.0 OD |
| <3% at 3.0 OD | |
| OD linearity | <1% from 0 to 3.0 OD |
| OD repeatability | <0.5% at 2.0 OD |
| Stray light | 0.03% at 230 nm |
| Reading speed (kinetic) | 96 well: 6 seconds |
| 384 well: 11 seconds | |
| 1536 well: 25 seconds | |
| FLUORESCENCE INTENSITY | |
| Light source | Xenon flash |
| Detector | Dual top PMTs |
| Single top PMT (option) | |
| Low noise PMT (bottom filter system) | |
| Red shifted PMT (top/bottom monochromator system) | |
| Wavelength selection | Quad monochromators (top/bottom) |
| Filters (top/bottom) | |
| Wavelength range | Monochromators: 250 – 850 nm |
| Filters (dual PMT): 200 – 850 nm | |
| Monochromator bandwidth | Variable, from 3 to 50 nm in 1 nm increments |
| Dynamic range | 7 decades |
| Sensitivity | Filters: |
| Fluorescein 0.2 pM (4 amol/well, 20 µL, 384-well low volume plate) – top | |
| Fluorescein 1 pM (10 amol/well, 10 µL, 1536-well plate) – top | |
| Fluorescein 1 pM (0.1 fmol/well, 100 µL, 384-well plate) – bottom | |
| Quad monochromator: | |
| Fluorescein 2 pM (40 amol/well, 20 µL, 384-well low volume plate) – top | |
| Fluorescein 2.5 pM (0.25 fmol/well, 100 µL, 384-well plate) – bottom | |
| Reading speed (kinetic) | 96 well: 6 seconds |
| 384 well: 11 seconds | |
| 1536 well: 25 seconds | |
| LUMINESCENCE | |
| Wavelength range | 300 – 700 nm |
| Dynamic range | > 6 decades |
| Sensitivity | 5 amol ATP typical (384-well low volume plate) |
| FLUORESCENCE POLARIZATION | |
| Light source | Xenon flash |
| Detector | Dual PMT or single PMT (option) |
| Wavelength selection | Filters |
| Wavelength range | 280 – 850 nm |
| Sensitivity | 1 mP standard deviation at 1 nM fluorescein |
| (384-well low volume plate) | |
| 1.5 mP standard deviation at 1 nM fluorescein (1536-well plate) | |
| TIME-RESOLVED FLUORESCENCE | |
| Light source | Xenon flash |
| Detector | Dual PMT or single PMT (option) |
| Wavelength selection | Quad monochromators (top/bottom) |
| Filters (top/bottom) | |
| Wavelength range | Monos: 250 – 850 nm |
| Filters (dual PMT): 200 – 850 nm | |
| Sensitivity | Europium 40 fM (384-well low volume plate) |
| Europium 70 fM (1536-well plate) | |
| ALPHA DETECTION | |
| Light source | 100 mW 680 nm laser |
| Detector | PMT |
| Wavelength selection | Filters (top) |
| Sensitivity | 100 amol LCK peptide (384-well low volume plate) |
| Read speed | 96 well: 30 seconds |
| 384 well: 1 minute 50 seconds | |
| 1536 well: 7 minutes 20 seconds | |
| REAGENT DISPENSERS | |
| Supported detection modes | All modes |
| Number | 2 syringe pumps |
| Supported labware | 6- to 384-well plates, Petri dishes |
| Dead volume | 1.1 mL with back flush |
| Dispense volume | 5 – 1000 µL in 1 µL increments |
| Dispense accuracy | + 1 µL or 2% |
| Dispense precision | < 2% at 50-200 µL |
| PHYSICAL CHARACTERISTICS | |
| Power | 250 Watts max. |
| Dimensions | 15.4″ W x 20.7″ D x 16.1″ H |
| (39 x 52.5 x 41 cm) | |
| Weight | 78 lbs (35 kg) |
| REGULATORY | |
| Regulatory | CE and TUV marked. RoHS Compliant. Models for In Vitro Diagnostic use are available. |
Micro-volume quantification is fast and easy with the Take3™ and Take3 Trio Micro-Volume plates, used in your BioTek microplate reader. Measure multiple 2 µL samples at a time, without diluting and without needing specialized equipment. Pre-programmed nucleic acid and protein protocols in Gen6 make quantification fast and easy.
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