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BioTek Synergy HTX

Short product description
Multi-Mode Microplate Reader


Synergy™ HTX Multi-Mode Microplate Reader is a compact, affordable system for 6- to 384-well microplates and Take3 Micro-Volume Plates. It’s unique dual optics design provides superior performance for UV-Vis absorbance, fluorescence, luminescence and AlphaScreen®/ AlphaLISA® workflows.

Incubation and shaking plus a dual reagent injector module to meet all your laboratory’s assay requirements now and in the future. Synergy HTX is controlled by the easy-to-use, yet powerful,  Gen 6 Software for data collection, analysis, exporting and reporting.

• Great flexibility at a low price
• Monochromator – based UV/Vis absorbance and filter-based fluorescence
• Linear and orbital shaking support and optimize many applications
• Excellent performance with AlphaScreen® and AlphaLISA®
• Dual Reagent Injectors are ideal for inject/read assays
  • Configurations

    Dual Reagent Injector*
    TRF (secondary mode)
    SoftwareGen6™ included with all configurations
  • Specifications

    Detection modesUV-Vis absorbance
    Fluorescence intensity
    Time-resolved fluorescence (secondary mode)
    Read methodsEnd point, kinetic, spectral scanning and well area scanning
    Microplate types6- to 384-well plates
    Other labware supportedPCR plates, Petri and cell culture dishes
    Take3 Micro-Volume Plates
    Temperature control4-Zone™ incubation to 50 °C with Condensation Control™
    +0.2 °C at 37 °C
    ShakingLinear, orbital
    SoftwareGen6 Data Analysis Software
    Gen5 Secure for 21 CFR Part 11 compliance (option)
    AutomationBioStack and 3rd party automation compatible
    Light sourceXenon flash
    Wavelength selectionmonochromator
    Wavelength range200 – 999 nm, 1 nm increments
    Monochromator bandwidth2.4 nm
    Dynamic range0 – 4.0 OD
    Resolution0.0001 OD
    Pathlength correctionyes
    Monochromator wavelength accuracy+2 nm
    Monochromator wavelength repeatability+0.2 nm
    OD accuracy<1% at 2.0 OD
    <3% at 3.0 OD
    OD linearity<1% from 0 to 3.0 OD
    OD repeatability<0.5% at 2.0 OD
    Stray light0.03% at 230 nm
    Reading speed (kinetic)96 wells: 14 seconds
    384 wells: 26 seconds
    Light sourceTungsten halogen
    Xenon flash (option)
    Wavelength selectionFilters
    Wavelength range300 – 700 nm (200 – 850 nm option)
    Dynamic range>6 decades
    SensitivityTop and Bottom: Fluorescein 5 pM (1 fmol/well 96-well plate)
    Reading speed (kinetic)96 wells: 31 seconds
    384 wells: 80 seconds
    Wavelength range300 – 700 nm
    Dynamic range>6 decades
    Sensitivity10 amol ATP (flash) – Lum and Abs / Lum configurations
    30 amol ATP (flash) – Multi-mode configurations
    Light sourceXenon flash
    Wavelength selectionMonochromator
    Light sourceTungsten halogen
    Wavelength selectionFilters
    Sensitivity300 amol of biotinylated-LCK-P peptide, 25 µL/ well in 384-well plate
    Read speed2 minute (96-well plate)
    Supported detection modesAll modes
    Number2 syringe pumps
    Supported labware6- to 384-well plates
    Dead volume1.1 mL with back flush
    Dispense volume5-1000 µL in 1 nm increments
    Dispense accuracy+1 µL or 2%
    Dispense precision<2% at 50 – 200 µL
    Connectivity1 USB, 1 RS232 for external PC control
    Power100-240 VAC, 50/60 Hz
    Dimensions16″W x 15″D x 10″H
    (40.6 x 38 x 25.4 cm)
    Weight40 lbs (18 kg)
    RegulatoryCE and TUV marked. RoHS Compliant. Models for In Vitro Diagnostic use are available.
  • Software

    Gen6 software controls all BioTek automated microplate readers, configuring system and data collection parameters, analysis and a variety of reporting and exporting features. One-click export to Excel® makes it fast and easy to export displayed data.
    Gen5 Secure software is available for GxP compliant laboratories with features that support 21 CFR Part 11 rules.

  • Micro Volume Plates

    Micro-volume quantification is fast and easy with the Take3™ and Take3 Trio Micro-Volume plates, used in your BioTek microplate reader. Measure multiple 2 µL samples at a time, without diluting and without needing specialized equipment. Pre-programmed nucleic acid and protein protocols in Gen5 make quantification fast and easy.

    • Low volume 2 µL nucleic acid and protein quantification
    • Pre-programmed protocols in Gen6 Software
    • Absorbance and fluorescence detection
    • Easy, wipe-off maintenance
    • Read 2 µL microspots, BioCells or standard cuvettes
  • Robotics

    BioTek’s robotic instruments can help automate workflows in your laboratory. Many life science workflows benefit from expanded automation, for increased throughput or process efficiency. BioTek offers unique automation solutions that integrate with our microplate washers, dispensers, readers and imagers. BioStack is a compact stacker that offers fast plate exchange options for 50 plates or more, and patented plate de-lidding and re-lidding with BioStack 4 . BioSpa 8 Automated Incubator links BioTek readers or imagers together with washers and dispensers for full workflow automation of up to 8 microplates. Temperature, CO2/O2 and humidity levels are controlled and monitored through the BioSpa software to maintain an ideal environment for cell cultures during all experimental stages.

  • Features

    Great flexibility at a low price
    Affordability doesn’t mean compromising on performance with the Synergy HTX. Its hardware design and Gen5-driven functionality address a wide range of multi-mode applications without costly and often unused options.

    Monochromator – based UV/Vis absorbance and filter-based fluorescence
    Synergy HTX provides great sensitivity with its filter-based fluorescence optics, and the monochromator-based absorbance optics offers 200 nm – 999 nm measurements without requiring filters.

    Linear and orbital shaking support and optimize many applications
    Assays that require shaking, such as bacterial and yeast growth studies, will benefit from the robust, programmable shaking options in Synergy HTX.

    Excellent performance with AlphaScreen® and AlphaLISA®
    The sensitive fluorescence optics in Synergy HTX provide great performance for many applications including important Alpha assays.

    Dual reagent injectors are ideal for inject/read assays
    Optional dual reagent injector module with angled tips, for inject/read applications, such as enzyme kinetics and Dual-Luciferase® Reporter. Synergy HTX is easily upgradable to add the dual reagent injector.


Brochure Multimode Microplate readers

Application notes

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Micro-volume Plates