The BioTek Synergy HTX is a compact, economical multimode plate reader for 6- to 384-well microplates and Take3 microvolume plates. The unique dual optics design provides superior performance for UV-Vis absorbance, fluorescence, luminescence, and AlphaScreen/AlphaLISA workflows.
With incubation and shaking, plus a dual reagent injector module, the system will meet all your assay requirements now and in the future. Synergy HTX is controlled by the easy-to-use, yet powerful, Agilent BioTek Gen6 data analysis software for data collection, analysis, exporting, and reporting.
Assays that require shaking, such as bacterial and yeast growth studies, will benefit from the robust, programmable shaking options in Synergy HTX. Select linear or orbital shaking, with programmable speed and duration.
| Part # | S1L | S1A | S1LA | S1LF | S1LFA | S1LFTA |
| Absorbance | • | • | • | • | ||
| Fluorescence | • | • | • | |||
| Luminescence | • | • | • | • | • | |
| Incubation | • | • | • | • | • | • |
| Shaking | • | • | • | • | • | • |
| Dual Reagent Injector* | • | • | • | • | • | • |
| TRF (secondary mode) | • | |||||
| Software | Gen6™ included with all configurations | |||||
| GENERAL | |
| Detection modes | UV-Vis absorbance |
| Fluorescence intensity | |
| Luminescence | |
| Time-resolved fluorescence (secondary mode) | |
| Alpha | |
| Read methods | End point, kinetic, spectral scanning and well area scanning |
| Microplate types | 6- to 384-well plates |
| Other labware supported | PCR plates, Petri and cell culture dishes |
| Take3 Micro-Volume Plates | |
| Temperature control | 4-Zone™ incubation to 50 °C with Condensation Control™ |
| +0.2 °C at 37 °C | |
| Shaking | Linear, orbital |
| Software | Gen6 Data Analysis Software |
| Gen5 Secure for 21 CFR Part 11 compliance (option) | |
| Automation | BioStack and 3rd party automation compatible |
| ABSORBANCE | |
| Light source | Xenon flash |
| Detector | photodiode |
| Wavelength selection | monochromator |
| Wavelength range | 200 – 999 nm, 1 nm increments |
| Monochromator bandwidth | 2.4 nm |
| Dynamic range | 0 – 4.0 OD |
| Resolution | 0.0001 OD |
| Pathlength correction | yes |
| Monochromator wavelength accuracy | +2 nm |
| Monochromator wavelength repeatability | +0.2 nm |
| OD accuracy | <1% at 2.0 OD |
| <3% at 3.0 OD | |
| OD linearity | <1% from 0 to 3.0 OD |
| OD repeatability | <0.5% at 2.0 OD |
| Stray light | 0.03% at 230 nm |
| Reading speed (kinetic) | 96 wells: 14 seconds |
| 384 wells: 26 seconds | |
| FLUORESCENCE INTENSITY | |
| Light source | Tungsten halogen |
| Xenon flash (option) | |
| Detector | PMT |
| Wavelength selection | Filters |
| Wavelength range | 300 – 700 nm (200 – 850 nm option) |
| Dynamic range | >6 decades |
| Sensitivity | Top and Bottom: Fluorescein 5 pM (1 fmol/well 96-well plate) |
| Reading speed (kinetic) | 96 wells: 31 seconds |
| 384 wells: 80 seconds | |
| LUMINESCENCE | |
| Wavelength range | 300 – 700 nm |
| Dynamic range | >6 decades |
| Sensitivity | 10 amol ATP (flash) – Lum and Abs / Lum configurations |
| 30 amol ATP (flash) – Multi-mode configurations | |
| TIME-RESOLVED FLUORESCENCE | |
| Light source | Xenon flash |
| Detector | PMT |
| Wavelength selection | Monochromator |
| ALPHA DETECTION | |
| Light source | Tungsten halogen |
| Detector | PMT |
| Wavelength selection | Filters |
| Sensitivity | 300 amol of biotinylated-LCK-P peptide, 25 µL/ well in 384-well plate |
| Read speed | 2 minute (96-well plate) |
| REAGENT DISPENSERS | |
| Supported detection modes | All modes |
| Number | 2 syringe pumps |
| Supported labware | 6- to 384-well plates |
| Dead volume | 1.1 mL with back flush |
| Dispense volume | 5-1000 µL in 1 nm increments |
| Dispense accuracy | +1 µL or 2% |
| Dispense precision | <2% at 50 – 200 µL |
| PHYSICAL CHARACTERISTICS | |
| Connectivity | 1 USB, 1 RS232 for external PC control |
| Power | 100-240 VAC, 50/60 Hz |
| Dimensions | 16″W x 15″D x 10″H |
| (40.6 x 38 x 25.4 cm) | |
| Weight | 40 lbs (18 kg) |
| REGULATORY | |
| Regulatory | CE and TUV marked. RoHS Compliant. Models for In Vitro Diagnostic use are available. |
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