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BioTek Synergy H1

Short product description
Hybrid Multi-Mode Reader. Choose monochromator-base optics for flexibility, filter-based optics for sensitivity, or both


Synergy™ H 1 is a configurable multi-mode microplate reader. You can choose monochromator-base optics for flexibility, filter-based optics for sensitivity, or both… Agilent’s patent Hybrid Technology™ offers high-performance and applications versatility in a modular platform to expand as your laboratory’s needs change. Top and bottom fluorescence intensity, UV-visible absorbance and luminescence detection are available to meet many life science research requirements.

  • Cost-effective hybrid offering excellent sensitivity, flexibility and value
  • Modular and upgradable: choose the modes you need now, add as needs change
  • Patented Hybrid Technology: sensitivity of filters and flexibility of monochromators
  • Low volume (2 µL) dsDNA quantification with Take3 Micro-Volume Plates
  • Live cell assay friendly with temperature control and CO2/O2 control


“At the end of last year we purchased four Synergy H1 readers in order to get a maximum of uniformity within the devices.
As a result, when running projects we have multiple options. ”

Patrick Janssen, Scientist Fermentation at NIZO

  • Specifications

    Detection modesUV-Vis absorbance
    Fluorescence intensity
    Fluorescence polarization
    Time-resolved fluorescence
    Read methodsEndpoint, kinetic, spectral scanning, well area scanning
    Microplate types6- to 384-well plates
    Other labware supportedPetri and cell culture dishes
    Take3 Micro-Volume Plates
    Temperature control4-Zone™ incubation to 45 °C with Condensation Control™
    +0.2 °C at 37 °C
    ShakingLinear, orbital, double orbital
    SoftwareGen6 Data Analysis Software
    Gen5 Secure for 21 CFR Part 11 compliance (option)
    AutomationBioStack and 3rd party automation compatible
    BioSpa 8 Automated Incubator compatible
    CO2 and O2 control (option)Range: 0 – 20% (CO2); 1 – 19% (O2)
    Control Resolution: +0.1% (CO2 and O2)
    Stability: +0.2% at 5% CO2; +0.2% at 1% O2
    Models for both CO2 and O2 or CO2 only are available
    Light sourceXenon flash
    Wavelength selectionmonochromator
    Wavelength range230 – 999 nm, 1 nm increments
    Monochromator bandwidth4 nm (230-285 nm), 8 nm (>285 nm)
    Dynamic range0 – 4.0 OD
    Resolution0.0001 OD
    Pathlength correctionyes
    Monochromator wavelength accuracy±2 nm
    Monochromator wavelength repeatability±0.2 nm
    OD accuracy<1% at 2.0 OD
    <3% at 3.0 OD
    OD linearity<1% from 0 to 3.0 OD
    OD repeatability<0.5% at 2.0 OD
    Stray light0.03% at 230 nm
    Reading speed (kinetic)96 wells: 11 seconds
    384 wells: 22 seconds
    Light sourceXenon flash
    DetectorPMT for monochromator system
    PMT for filter system
    Wavelength selectionQuad monochromators (top/bottom)
    Filters (top)
    Wavelength rangeMonochromators: 250 – 700 nm (850 nm option)
    Filters: 200 – 700 nm (850 nm option)
    Monochromator bandwidthFixed, 16 nm
    Dynamic range7 decades
    Fluorescein 0.25 pM (0.025 fmol/well, 384-well plate)
    Quad Monochromator:
    Fluorescein 2.5 pM (0.25 fmol/well, 384-well plate) – top
    Fluorescein 4 pM (0.4 fmol/well, 384-well plate) – bottom
    Reading speed (kinetic)96 wells: 11 seconds
    384 wells: 22 seconds
    Wavelength range300 – 700 nm
    Dynamic range>6 decades
    SensitivityMonos: 20 amol ATP (flash)
    Filters: 10 amol ATP (flash), 100 amol (glow)
    Light sourceXenon flash
    Wavelength selectionFilters
    Wavelength range280 – 700 nm (850 nm option)
    Sensitivity1.2 mP standard deviation at 1 nm fluorescein
    Light sourceXenon flash
    Wavelength selectionQuad monochromators (secondary mode)
    Filters (top)
    Wavelength rangeFilters: 200 – 700 nm (850 nm option)
    SensitivityFilters: Europium 40 fM (4 amol/well, 384-well plate)
    Monos: Europium 1200 fM (120 amol/well, 384-well plate)
    Supported detection modesAll modes
    Number2 syringe pumps
    Supported labware6- to 384-well microplates, Petri dishes
    Dead volume1.1 mL with back flush
    Dispense volume5 – 1000 µL in 1 µL increment
    Dispense accuracy±1 µL or 2%
    Dispense precision<2% at 50-200 µL
    Power130 Watts max.
    Dimensions15.4″W 18.6″D 12.9″H
    (39.1 x 47.2 x 32.8 cm)
    Weight50 lbs (22.5 kg)
    RegulatoryCE and TUV marked. RoHS Compliant. Models for In Vitro Diagnostic use are available.
  • Software

    Gen6 software controls all Agilent BioTek automated microplate readers, configuring system and data collection parameters, analysis and a variety of reporting and exporting features. One-click export to Excel® makes it fast and easy to export displayed data.
    Gen5 Secure software is available for GxP compliant laboratories with features that support 21 CFR Part 11 rules.

  • Micro Volume Plates

    Micro-volume quantification is fast and easy with the Take3™ and Take3 Trio Micro-Volume plates, used in your BioTek microplate reader. Measure multiple 2 µL samples at a time, without diluting and without needing specialized equipment. Pre-programmed nucleic acid and protein protocols in Gen6 make quantification fast and easy.

    • Low volume 2 µL nucleic acid and protein quantification
    • Pre-programmed protocols in Gen6 Software
    • Absorbance and fluorescence detection
    • Easy, wipe-off maintenance
    • Read 2 µL microspots, BioCells or standard cuvettes
  • Robotics

    Agilent’s robotic instruments can help automate workflows in your laboratory. Many life science workflows benefit from expanded automation, for increased throughput or process efficiency. Agilent offers unique automation solutions that integrate with our microplate washers, dispensers, readers and imagers.

    BioStack is a compact stacker that offers fast plate exchange options for 50 plates or more, and patented plate de-lidding and re-lidding with BioStack 4 .

    BioSpa 8 Automated Incubator links Agilent readers or imagers together with washers and dispensers for full workflow automation of up to 8 microplates. Temperature, CO2/O2 and humidity levels are controlled and monitored through the BioSpa software to maintain an ideal environment for cell cultures during all experimental stages.

  • Features

    Cost-effective hybrid offering excellent sensitivity, flexibility and value
    Agilent’s Hybrid Technology offers affordable multi-mode detection without compromising on performance. Sensitive filter-based optics, flexible monochromator-based optics, or both – on one instrument, powered by Gen6 software for data collection and analysis.

    Modular and upgradable: choose the modes and modules you need now, add as needs change
    With six different base configurations to choose from, Synergy H1 is an affordable, upgradable solution to address your laboratory’s research needs now and well into the future

    Patented Hybrid Technology™: sensitivity of filters and flexibility of monochromators
    Synergy H1’s hybrid optical design offers filter-based fluorescence optics for high transmission and high sensitivity, while the available monochromator optics offers convenience and assay flexibility. Multiple fluorescence measurement modes include fluorescence intensity, time-resolved fluorescence and fluorescence polarization

    Low volume (2 µL) dsDNA quantification with Take3 Micro-Volume Plates
    The Take3 Micro-Volume plate extends the Synergy H1 capabilities beyond standard microplate reading, by measuring up to 48, 2 µL samples at one time for rapid nucleic acid and protein quantification. Absorbance and fluorescence measurements are quick and easy, no additional instrumentation is required!

    Live cell assay friendly with temperature control and CO2/O2 control
    Synergy H1 offers temperature control to 45 °C, plus linear and orbital shaking. Expand the applications base to live cell assays by adding the CO2/02 controller or the dual reagent injector for quick inject/read processes.

    Gen6 Data Analysis Software; Gen5 Secure for 21 CFR Part 11 compliance
    Gen6 Microplate Reader and Imager Software doesn’t compromise on usability or data analysis performance – protocol creation is intuitive and flexible, and the data analysis capability rivals standalone packages. For the GxP lab, Gen5 Secure software offers features to help comply with 21 CFR Part 11, including electronic signature, data security and multiple level permissions.


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Application notes

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Automated Incubator
Microplate stacker
Micro-volume Plates