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BioTek Cytation 1

Short product description
Cell Imaging Multi-Mode Reader. Combine fluorescence and high contrast brightfield imaging with conventional multi-mode detection


Cytation1 Cell Imaging Multi-Mode Reader combines fluorescence and high contrast brightfield imaging with conventional multi-mode detection without the expense and complexity commonly associated with other digital microscopy systems. This unique, patented design provides both quantitative phenotypic cellular information with well-based quantitative data. Cytation 1’s multi-mode detection module includes high sensitivity filter-based fluorescence and a monochromator system for UV-Vis absorbance. Temperature control and shaking are standard; CO2/O2 control and reagent injectors are available. BioTek’s powerful Gen5 software makes image capture and plate reading easy.

  • Affordable, patented, quantitative digital microscopy and optional multi-mode microplate detection
  • Augmented Microscopy™ using Gen5 software for seamless image capture to quantitative publication ready data with no extensive training required
  • Fluorescence and high contrast brightfield imaging from 1.25x to 60x to capture large regions of interest to 60x or intracellular details
  • Affordable automation: automated XY stage, focus, exposure, image capture and LED intensity
  • Cell friendly design – 4-Zone incubation to 45°C with Condensation Control, CO2/O2 control, angled injectors and lidded plate handling
  • High performance filter-based fluorescence detection with convenience of monochromator based UV-Vis absorbance


Have you read the testimonial of Joëlle J.E. Janssen about how she used the Cytation 1 during her PhD? Read it here: The Cytation 1 enabled me to obtain high-quality brightfield images of white blood cells

  • Configurations


    Imaging Plus Multi-Mode Configurations


    Fluorescence and high contrast brightfield imaging
    Monochromator absorbance
    Filter/dichroic fluorescence
    Luminescence / filtered luminescence
  • Specifications

    Detection modesUV-Vis absorbance
    Fluorescence intensity
    Fluorescence polarization
    Time-resolved fluorescence
    Read methodsEndpoint, kinetic, spectral scanning, well area scanning
    Microplate typesMonochromator: 6- to 384-well plates
    Filters: 6- to 1536-well plates
    Imaging: 6- to 1536-well plates
    Other labware supportedMicroscope slides, Petri and cell culture dishes, cell culture flasks (T25), counting chambers (hemocytometer)
    Take3 Micro-Volume Plates
    Temperature control4-Zone incubation to 45 °C with Condensation Control™; Variation +0.2 °C at 37 °C
    ShakingLinear, orbital, double orbital
    SoftwareGen5™ Microplate Reader and Imager Software
    Gen5 Image+ and Image Prime software available for full image analysis
    Gen5 Secure available for 21 CFR Part 11
    AutomationBioStack and 3rd party automation compatible
    BioSpa 8 Automated Incubator compatible
    CO2 and O2 control (option)0 – 20% CO2 control and 1 – 19% O2 control, with optional Gas Controller
    Models for both CO2 and O2 or CO2 only are available
    Imaging modeFluorescence
    High Contrast Brightfield (2.5x Zeiss, 4x, 10x, 20x, 40x and 60x)
    Imaging methodSingle color, multi-color, montage, time lapse, z-stacking
    Image processingZ-projection, image stitching
    Camera16-bit gray scale, Sony CCD
    Objective capacity2 user-replaceable objectives
    Objectives available1.25x, 2.5x (2.25x eff),2.5x (2.75x eff), 4x,10x, 20x, 40x, 60x
    Image filter cube capacity4 user-replaceable fluorescence cubes plus brightfield channel
    Imaging filter cubes availableDAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7,Acridine Orange (ACR OR), CFP-YFP FRET, propidium,Iodide, chlorophyll, phycoerythrin, CY5.5, TagBFP, Alexa568, Ex377 / Em647
    Imaging LED cubes available365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm
    Automated functionsAutofocus, auto LED intensity, auto exposure
    Autofocus methodImage-based autofocus
    User-trained autofocus
    Laser autofocus (option)
    Positional controlsSoftware control
    Image collection rateImage-based autofocus:
    96 wells, 1 color (DAPI), 4x, 6 minutes
    96 wells, 3 colors, 4x, 12 minutes
    Laser autofocus:
    96 wells, 1 color (DAPI), 4x, <3 minutes
    96 wells, 3 colors, 4x, <7 minutes, 30 seconds Burst Mode: 10 fps, single well, single color at <= 50ms integration time
    Image Analysis Software optionGen5 Image+: Image analysis
    Gen5 Image Prime: Advanced image analysis
    Gen5 Secure Image+: Advanced image analysis, 21 CFR Part 11 features
    Light sourceXenon flash
    Wavelength selectionDeep blocking band pass filters / dichroic mirrors
    Wavelength rangeFilters: 200 – 700 nm (850 nm option)
    Dynamic range7 decades
    SensitivityFluorescein: 0.25 pM (0.025 fmol/well, 384-well plate)
    Reading speed (kinetic)96 wells: 11 seconds
    384 wells: 22 seconds
    Wavelength range300 – 700 nm
    Dynamic range>6 decades
    Sensitivity10 amol ATP (flash)
    100 amol (glow)
    Light sourceXenon flash
    Wavelength selectionFilters
    Wavelength range400 nm – 700 nm
    Sensitivity1.2 mP standard deviation at 1 nM fluorescein
    Light sourceXenon flash
    Wavelength selectionFilters
    SensitivityEuropium 40 fM (4 amol/well, 384-well plate)
    Light sourceXenon flash
    Wavelength selectionMonochromator
    Wavelength range200 – 999 nm, 1 nm increment
    Monochromator bandwidth2.4 nm
    Dynamic range0 – 4.0 OD
    Resolution0.0001 OD
    Pathlength correctionyes
    Monochromator wavelength accuracy+ 2 nm
    Monochromator wavelength repeatability+ 0.2 nm
    OD accuracy<1% at 2.0 OD
    <3% at 3.0 OD
    OD linearity<1% from 0 to 3.0 OD
    OD repeatability<0.5% at 2.0 OD
    Stray light0.03% at 230 nm
    Reading speed (kinetic)96 wells: 11 seconds
    384 wells: 22 seconds
    Supported detection modesAll modes
    Number2 syringe pumps
    Supported labware6- to 384-well plates, Petri and cell culture dishes
    Dead volume1.1 mL with back flush
    Dispense volume5 – 1000 µL in 1 µL increments
    Plate geometry6- to 384-well microplates
    Dispense accuracy+1 µL or 2%
    Dispense precision<2% at 50 – 200 µL
    Power100-240 VAC, 50/60 Hz (24VDC external power supply, 150W min)
    Dimensions20″ D x 16.5″ W x 17.5″ H
    (50.8 cm x 41.91 cm x 44.5 cm)
    Weight65 lbs (29 Kg)
    RegulatoryCE and TUV marked. Models for In Vitro Diagnostic use are available.
  • Peltier Cooling Module

    The Peltier Cooling Module for Agilent’s Cytation™ 5 and Cytation 1 Cell Imaging Multi-Mode Readers helps maintain temperature stability inside the reading chamber, regardless of changes in ambient (room) temperature and heat produced by electro-mechanical operations within the reader.
    The compact Peltier Cooling Module attaches easily to Cytation’s rear case. (See image)

    Instrument without Peltier Cooling Module:

    The instrument’s interior temperature (red) follows ambient room temperature (green). It is also significantly warmer than ambient temperature because of heat produced by the instrument. This can result in assay data variation through the day, or drifts for kinetic assays run at ambient temperature
    Instrument with Peltier Cooling Module:

    The instrument’s interior temperature (blue) is stabilized which results in more consistent assay data, and higher quality room temperature kinetic data

    Benefits of the Peltier Cooling Module for Cytation:
    • Helps maintain the instrument’s internal ambient temperature stability, with less than 1 °C rise over external ambient, enabling more consistent results
    • Quickly reduces internal temperature following incubated assays up to 3x faster, to allow both incubated and ambient assays to be run more efficiently in multi-user environments.

    Peltier Cooling Module is available for:
    • Cytation Multi-Mode Reader
    • Cytation 5 Cell Imaging Multi-Mode Reader
    • Cytation 1 Cell Imaging Multi-Mode Reader

  • Features

    Collect both phenotypic data and quantitative data from one instrument. Image and data analysis are provided with Gen5 software for all applications

    Patented Hybrid optics: sensitivity of filters, flexibility of monochromator
    Cytation 5’s hybrid optical design offers filter-based fluorescence optics for high transmission and high sensitivity, while the available monochromator optics offers convenience and assay flexibility. Multiple fluorescence measurement modes include fluorescence intensity, time-resolved fluorescence and fluorescence polarization

    Augmented Microscopy™ using Gen5 software: image capture to quantitative publication ready data.
    Gen5 software takes you automatically through the steps typically performed in microscopy: capture > process > analyze > publish. The interface is logical and user-friendly, so your imaging workflows can be up and running without extensive training.

    Fluorescence and high contrast brightfield imaging
    The powerful imaging module in Cytation 1 offers four color channels plus brightfield, from 1.25x for imaging larger samples to 60x for intracellular details. More than 15 filter/LED cubes are available, supporting a broad range of imaging applications.

    Affordable automation
    Cytation 1 has an automated XY stage, autofocus, auto exposure and auto LED intensity for efficient capture of high quality images. The automated features in Cytation 1 and Gen5 make it easy for you to find the region of interest, then quickly find the best settings to capture the images. Gen5 software is simple to use, without extensive training.

    Cell friendly design
    Live cell assays require an optimal environment. Cytation 1 has temperature control and shaking, plus CO2 / O2 control, to facilitate longer-term kinetic studies.

    High performance and convenience
    To optimize multi-mode detection, Cytation 1 has high performance filters for fluorescence and monochromator-based UV-Vis absorbance for convenient wavelength selection in 1 nm increments.


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