The xCELLigence RTCA SP instrument uses biosensors to monitor in real-time cell proliferation & viability, biofilm formation & dissipation, and cytophathic effects in a label-free manner. Seed cells and walk away – staining, washing, and spectrophotometric analysis are eliminated. Data is continuously recorded from minutes to days, even weeks.
The instrument operates in a standard CO2 cell culture incubator and the control unit is housed outside the incubator. User friendly software allows for real-time control and monitoring of the instrument with real-time data analysis functions.
|Immune Cell Killing
|Virus Cytopathic Effects
|Operating Environment Relative Humidity
|Operating Environment Temperature
How is xCELLigence RTCA used for killing assays? The xCELLigence Real-Time Cell Analysis (RTCA) instrument monitors cell killing in real time. Simply add target and immune effector cells to the patented microtiter plates (E-Plates), load the instrument, and start reading. The biosensors embedded in the bottom of each well allow for the automated kinetic measurement of changes in cell number, size, and substrate attachment quality.
Target cell death can be measured continuously and automatically from hours to days without the use of labels.
Cellular Impedance Explained
Positioned between reductionistic biochemical assays and whole organism in vivo experimentation, cell-based assays serve as an indispensable tool for basic and applied biological research. However, the utility of many cell-based assays is diminished by: (1) the need to use labels, (2) incompatibility with continuous monitoring (i.e. only end point data is produced), (3) incompatibility with orthogonal assays, and (4) the inability to provide an objective/quantitative readout. Each of these shortcomings is, however, overcome by the non-invasive, label-free, and real-time cellular impedance assay.
The xCELLigence RTCA SP instrument records data continuously over the course of minutes, days, or weeks. Assays often performed on this instrument are cell proliferation and viability applications, for vaccine and anti-infective development. This is done in a 96-well format in a simple and label-free manner. The instrument operates in a standard CO2 cell culture incubator and the control unit is housed outside the incubator. User friendly software allows for real-time control and monitoring of the instrument with real-time data analysis functions
• Cancer immunotherapy:
With the monitoring of cell killing
• Virology & Infectious diseases:
Monitor pathogens behaviour
• Cell signalling:
Capture changes in cell behaviour due to signalling
• Cytotoxicity Overview:
Monitor cell behaviour and attachment to the plate surface
• Cell Barrier Function:
Continuous monitoring of the cell barrier
• Cell Adhesion:
Studying cell adhesion and cell spreading
Please login to be able to see the application notes.