The xCELLigence RTCA MP instrument uses biosensors to continuously monitor cell behavior in a 6 x 96-well format. Obtain continuous cell assay data points using label-free methods. This multiple plate format is ideal for cell killing and immunotherapy applications such as antibody dependent cytotoxicity, bispecific T cell engagers, checkpoint inhibitors, NK cells, T cells, B cells, oncolytic viruses.
The MP model can host up to six 96-well electronic microplates (E-Plate 96) which can be run in parallel or independently of one another. This flexible plate batch processing allows for maximum productivity for multiple users. The instrument operates in a standard CO2 cell culture incubator and the control unit is housed outside the incubator. Easy-to-use intuitive software allows for real-time control and monitoring of the instrument and includes real-time data display and analysis functions.
Combining impedance with microscopy?
check out the Agilent xCELLigence RTCA eSight.
|Analyzer Application||Adhesion||Apoptosis||Cell Characterization|
|Cytotoxity||Immune Cell Killing||Proliferation|
|Receptor Signaling||Stem Cells||Virus Cytopathic Effects|
|Operating Environment Relative Humidity||5-98 %|
|Operating Environment Temperature||20-40 °C|
|Sampling Format||6 x 96 wells plates|
How is xCELLigence RTCA used for killing assays? The xCELLigence Real-Time Cell Analysis (RTCA) instrument monitors cell killing in real time. Simply add target and immune effector cells to the patented microtiter plates (E-Plates), load the instrument, and start reading. The biosensors embedded in the bottom of each well allow for the automated kinetic measurement of changes in cell number, size, and substrate attachment quality.
Target cell death can be measured continuously and automatically from hours to days without the use of labels.
Cellular Impedance Explained
Positioned between reductionistic biochemical assays and whole organism in vivo experimentation, cell-based assays serve as an indispensable tool for basic and applied biological research. However, the utility of many cell-based assays is diminished by: (1) the need to use labels, (2) incompatibility with continuous monitoring (i.e. only end point data is produced), (3) incompatibility with orthogonal assays, and (4) the inability to provide an objective/quantitative readout. Each of these shortcomings is, however, overcome by the non-invasive, label-free, and real-time cellular impedance assay.
The xCELLigence RTCA MP instrument obtains continuous data from cell behaviour in a multiple plate format: i.e. 6 x 96 wells. The plates can run independently of each other or in parallel, providing maximum efficiency during the research. In a real-time manner, the analyser offers data of multiple read-outs, including cell number, proliferation rate, cell size/shape and cell-substrate attachment quality. The instrument operates in a standard CO2 cell culture incubator and the control unit is housed outside the incubator. Easy-to-use intuitive software allows for real-time control and monitoring of the instrument and includes real-time data display and analysis functions.
• Cancer immunotherapy:
With the monitoring of cell killing
• Virology & Infectious diseases:
Monitor pathogens behaviour
• Cell signalling:
Capture changes in cell behaviour due to signalling
• Cytotoxicity Overview:
Monitor cell behaviour and attachment to the plate surface
• Cell Barrier Function:
Continuous monitoring of the cell barrier
• Cell Adhesion:
Studying cell adhesion and cell spreading