The xCELLigence RTCA DP (dual purpose) instrument is unique in the ability to make continuous measurements of cell invasion and migration (CIM) using an electronically integrated Boyden chamber.
The instrument uses electrical impedance monitoring to quantify changes with a label-free methodology. The three cradles of the DP instrument enable three separate electronic 16-well plates to be controlled and monitored in parallel or independently of one another. This flexible plate batch processing allows maximum productivity for multiple users. The instrument operates in a standard CO2 cell culture incubator and the control unit is housed outside the incubator.
|Analyzer Application||Adhesion||Apoptosis||Cell Characterization|
|Cytotoxity||Immune Cell Killing||Proliferation|
|Receptor Signaling||Stem Cells||Virus Cytopathic Effects|
|Cell Invasion & Migration|
|Operating Environment Relative Humidity||5-98 %|
|Operating Environment Temperature||20-40 °C|
|Sampling Format||3 x 16 well plates|
Cell invasion and migration plate
The cell invasion and migration plate (CIM-Plate 16) is a 16-well electronically integrated Boyden chamber, composed of upper and lower chambers that snap together (Figure 2). Pressure-sensitive silicone O-rings are present in the lower chamber to ensure a tight seal between the upper and lower chambers for each well (Figure 2C). A PET microporous membrane serves as the base of the upper chamber, allowing cells to translocate towards chemoattractant in the lower chamber (Figure 2B). The bottom side of the membrane is coated with gold biosensors (Figure 2B) that have an ability to detect the reduced electric current when cells adhere to their surface. This “impedance” signal enables a quantitative kinetic measurement of cell movement from the upper chamber to the lower chamber.
CIM-Plate overview. (A) CIM-Plate components. (B) A fully assembled CIM-Plate in detail. The expanded view illustrates the upper and lower chambers of a single well. Cells can migrate through the bottom membrane of the upper chamber and gold electrodes on the underside of this membrane detect the presence of adherent cells. (C) Gold biosensors are coated in the bottom surface of the upper chamber, composed of a PET microporous membrane. For a simple migration assay (not illustrated here), the cells being monitored would be plated directly onto the membrane. For an invasion assay (shown here), cells are plated on top of either the basement membrane matrix or a monolayer of cells.
Cellular Impedance Explained
Positioned between reductionistic biochemical assays and whole organism in vivo experimentation, cell-based assays serve as an indispensable tool for basic and applied biological research. However, the utility of many cell-based assays is diminished by: (1) the need to use labels, (2) incompatibility with continuous monitoring (i.e. only end point data is produced), (3) incompatibility with orthogonal assays, and (4) the inability to provide an objective/quantitative readout. Each of these shortcomings is, however, overcome by the non-invasive, label-free, and real-time cellular impedance assay.
The xCELLigence RTCA DP system is comparable to all other xCELLigence RTCA system and offers some extra capabilities too! The instrument is unique in its capability to use electrical impedance to quantify Cell Invasion and Migration (CIM). The electronically integrated Boyden chamber gives a signal that correlates with the degree of cell migration/invasion. It enables researchers to run 3 x 16 well plates independently in a label-free manner. The instrument operates in a standard CO2 cell culture incubator and the control unit is housed outside the incubator. Easy-to-use intuitive software allows for real-time control and monitoring of the instrument and includes real-time data display and analysis functions.
• Cancer immunotherapy:
With the monitoring of cell killing
• Virology & Infectious diseases:
Monitor pathogens behaviour
• Cell Invasion & Migration:
Due to the integrated Boyden chambers
• Cytotoxicity Overview:
Monitor cell behaviour and attachment to the plate surface
• Stem Cell Differentiation:
Capture the process of stem cells differentiating into somatic cells
• Cell Adhesion:
Studying cell adhesion and cell spreading