The Lionheart LX Automated Microscope is designed with affordability in mind, offering an alternative to expensive, custom-built automated microscopes that are complex to learn and use. The compact, ocular-free hardware design eases ergonomic discomfort. Along with Gen5™ software, Lionheart LX enables Augmented Microscopy™, which fully automates image capture, processing and analysis. Load samples, start a run and come back to publication-ready images and quantitative data. Lionheart LX can help increase your research output while reducing processing time and costs.
GENERAL | |
Microplate types | 6- to 1536-well plates |
Other labware supported | Microscope slides, Petri and cell culture dishes, cell culture flasks (T25, T75), counting chambers (hemocytometers), chamber slides |
Support for labware up to 1.5″ tall | |
Software | Gen5 Microplate Reader and Imager Software included |
Gen5 Image and Image+ Software available for advanced image analysis (option) | |
X/Y Stage resolution | Lead screw driven stage with 0.1 micron resolution |
IMAGING SYSTEM | |
Imaging mode | Fluorescence, high contrast brightfield and color brightfield |
Imaging method | Single color, multi-color, montage, time lapse, Z-stack, z-stack montage, burst mode |
Light source | High power LEDs (available wavelengths: 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm) |
Camera | 16-bit CMOS camera. 2.3 MP Sony IMX249 1/1.2″ sensor with 5.86 micron pixel size |
Camera binning | Optional 2×2 binning for focus and/or image capture |
Camera exposure range | 5 milliseconds to 4 seconds |
Image outputs available | Raw Images: 16-bit TIFF |
Saved Images: TIFF, JPG, BMP, PNG, EMF, GIF, color TIFF | |
Movies: MP4, WMV | |
Objective capacity | 6 onboard, user-replaceable objectives |
Objectives available | Air: 1.25x, NA .04; 2.5x (2.25x eff), NA: .07; 2.5x (2.75x eff), NA: .12; 4x, NA: .13; 10x, NA: .30; 20x, NA: .45; 40x, NA.60; 60x, NA .70 |
Oil: 60x, NA:1.42; 60x, NA 1.25; 100x, NA 1.4; 100x, NA 1.3 | |
High NA: 20x, NA 0.75; 40x, NA 0.95 | |
Image filter cube capacity | 4 user-replaceable fluorescence cubes plus brightfield channel |
Imaging filter cubes available | DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7, Acridine Orange, CFP-YFP FRET, Chlorophyll, Phycoerythrin (PE), Propidium Iodide, CY5.5, TagBFP, GFP (Ex)-CY6 (Em), RFP (Ex)-CY5 (Em), Alexa 568, Ex377 / Em647 |
Automated functions | Autofocus, user-trained autofocus, auto exposure, auto-LED intensity |
Autofocus method | Image-based autofocus |
Laser autofocus option | |
Image collection rate | Single well fastest frame rate capture: |
*Full Resolution: up to 10 frames per second for single color images | |
*2×2 Binning: up to 20 frames per second for single color images | |
Microscope stage control | Gen5 Software Control |
Optional joystick controller | |
PHYSICAL CHARACTERISTICS | |
Power | 250 Watts max. |
Dimensions | 18.3″ D, 17.9″ W, 14.1″ H (46.5 cm x 45.5 cm x 35.8 cm) |
Weight | 51 lbs (23.1 kg) |
REGULATORY | |
Regulatory | CE and TUV marked. RoHS compliant. Models for In Vitro Diagnostic use are available. |
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