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BioTek Lionheart LX

Short product description
Automated digital endpoint microscope


The Lionheart LX Automated Microscope is designed with affordability in mind, offering an alternative to expensive, custom-built automated microscopes that are complex to learn and use. The compact, ocular-free hardware design eases ergonomic discomfort. Along with Gen5™ software, Lionheart LX enables Augmented Microscopy™, which fully automates image capture, processing and analysis. Load samples, start a run and come back to publication-ready images and quantitative data. Lionheart LX can help increase your research output while reducing processing time and costs.


  • Affordable automated digital microscopy
  • High contrast brightfield, color brightfield and fluorescence imaging

  • Augmented Microscopy: Easy imaging workflow automation

  • Quick analyze: instant cell count and confluence measurement
  • Integrated, compact design offers quick installation and setup
  • Small benchtop footprint
  • Specifications

    Microplate types6- to 1536-well plates
    Other labware supportedMicroscope slides, Petri and cell culture dishes, cell culture flasks (T25, T75), counting chambers (hemocytometers), chamber slides
    Support for labware up to 1.5″ tall
    SoftwareGen5 Microplate Reader and Imager Software included
    Gen5 Image and Image+ Software available for advanced image analysis (option)
    X/Y Stage resolutionLead screw driven stage with 0.1 micron resolution
    Imaging modeFluorescence, high contrast brightfield and color brightfield
    Imaging methodSingle color, multi-color, montage, time lapse, Z-stack, z-stack montage, burst mode
    Light sourceHigh power LEDs (available wavelengths: 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm)
    Camera16-bit CMOS camera. 2.3 MP Sony IMX249 1/1.2″ sensor with 5.86 micron pixel size
    Camera binningOptional 2×2 binning for focus and/or image capture
    Camera exposure range5 milliseconds to 4 seconds
    Image outputs availableRaw Images: 16-bit TIFF
    Saved Images: TIFF, JPG, BMP, PNG, EMF, GIF, color TIFF
    Movies: MP4, WMV
    Objective capacity6 onboard, user-replaceable objectives
    Objectives availableAir: 1.25x, NA .04; 2.5x (2.25x eff), NA: .07; 2.5x (2.75x eff), NA: .12; 4x, NA: .13; 10x, NA: .30; 20x, NA: .45; 40x, NA.60; 60x, NA .70
    Oil: 60x, NA:1.42; 60x, NA 1.25; 100x, NA 1.4; 100x, NA 1.3
    High NA: 20x, NA 0.75; 40x, NA 0.95
    Image filter cube capacity4 user-replaceable fluorescence cubes plus brightfield channel
    Imaging filter cubes availableDAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7, Acridine Orange, CFP-YFP FRET, Chlorophyll, Phycoerythrin (PE), Propidium Iodide, CY5.5, TagBFP, GFP (Ex)-CY6 (Em), RFP (Ex)-CY5 (Em), Alexa 568, Ex377 / Em647
    Automated functionsAutofocus, user-trained autofocus, auto exposure, auto-LED intensity
    Autofocus methodImage-based autofocus
    Laser autofocus option
    Image collection rateSingle well fastest frame rate capture:
    *Full Resolution: up to 10 frames per second for single color images
    *2×2 Binning: up to 20 frames per second for single color images
    Microscope stage controlGen5 Software Control
    Optional joystick controller
    Power250 Watts max.
    Dimensions18.3″ D, 17.9″ W, 14.1″ H (46.5 cm x 45.5 cm x 35.8 cm)
    Weight51 lbs (23.1 kg)
    RegulatoryCE and TUV marked. RoHS compliant. Models for In Vitro Diagnostic use are available.
  • Configurations

    Lionheart LX Automated Microscope. Fluorescence, high contrast brightfield and color brightfield imaging. Includes standard Gen5 software and 4-position slide holder. The imaging controller, objectives, filter/LED cubes, accessories and software upgrades are sold separately.

  • Software

    Gen5 Microplate Reader and Imager Software controls Lionheart LX for automated image capture, processing, analysis and development of publication-ready images and data. This imaging and microscopy automation represents BioTek’s Augmented Microscopy™ – the simplified, automated process to accomplish multiple microscopy steps in a single platform. Gen5 is available in several editions to meet the image analysis requirements across a range of applications.

    Gen5 Image+ offers complete imaging control for Lionheart LX Automated Microscope, and provides image processing and analysis including z-projection, image stitching, hitpicking and digital phase contrast.

    Gen5 Image Prime controls Lionheart LX Automated Microscope includes more expansive image processing and analysis: Z-projection, image stitching, hitpicking and digital phase contrast, plus advanced features including secondary mask, dynamic thresholding and subpopulation analysis.

    Gen5 Secure editions add features to help ensure compliance to 21 CFR Part 11 requirements.

    Gen5 add-on modules provide increased functionality for specific applications or analyses, such as Spot Counting.

  • Features

    Affordable automated digital microscopy.
    Lionheart LX has an automated 6-objective turret and can contain up to 4 LED/filter cubes for multi-channel image capture. The precise automated stage and autofocus, auto exposure and other Gen5 software features enable simple, automated image capture and analysis.

    High contrast brightfield, color brightfield and fluorescence imaging
    With brightfield, color brightfield and fluorescence imaging in four channels – and more than twenty available filter/LED cubes, Lionheart LX offers fantastic versatility that isn’t limited to image capture. Imaging processes from z-stacking to z-projection, montage collection to stitching; plus analysis from label-free cell counting to mitochrondrial membrane potential are all accomplished with Lionheart LX and Gen5 software.

    Augmented Microscopy: Easy imaging workflow automation
    BioTek’s Augmented Microscopy automates image capture, processing and analysis for publication-ready images and data. Automated image capture starts with image-based and laser autofocus, plus auto LED and exposure. Automated image pre-processing optimizes images for downstream analysis, from cell counting to characterization of subcellular details

    Quick analyze: instant cell count and confluence measurement
    Get cell counts and confluence calculations directly on the live camera feed. Gen5 software controls Lionheart LX to quickly find the region of interest and display counts onscreen without needing to capture the image. The quick analyze function works for samples in a variety of labware.

    Integrated, compact design offers quick installation and setup
    Lionheart LX integrates all microscopy hardware into a very compact footprint, saving valuable bench space. The easily accessible objectives and LED/filter cubes make setup and operation fast and simple, with a minimal learning curve.


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