BioTek Lionheart FX

Agilent

BioTek Lionheart FX

Short product description

Compact automated live-cell microscope up to 100x

Information

Lionheart™ FX Automated Microscope is a compact, inclusive microscopy system for a broad range of imaging workflows.

It offers up to 100x air and oil immersion magnification, with:

Fluorescence,
Brightfield,
Color brightfield,
and phase contrast channels for maximum application reach.

An optional environment control cover provides incubation to 40°C and effective containment for CO2/O2 control, a humidity chamber optimizes conditions for long-term live cell imaging applications, and an available dual reagent injector facilitates rapid kinetic assays. Lionheart FX and Gen5 together comprise Augmented Microscopy™ – the automation of image capture, processing, analysis and development of publication-ready images and data.

Lionheart FX vs Lionheart LX

	Lionheart FX	Lionheart LX

General
Microplate types	6- to 1536-well plates	6- to 1536-well plates
Other labware supported	Microscope slides, Petri and cell culture dishes, cell culture flasks (T25, T75), counting chambers (hemocytometers), chamber slides	Microscope slides, Petri and cell culture dishes, cell culture flasks (T25, T75), counting chambers (hemocytometers), chamber slides

	Support for labware up to 1.5″ tall	Support for labware up to 1.5″ tall
Temperature control	Incubation to 40 °C with optional environmental control cover
Software	Gen5 Microplate Reader and Imager Software included	Gen5 Microplate Reader and Imager Software included

	Gen5 Image and Image+ Software available for advanced image analysis (option)	Gen5 Image and Image+ Software available for advanced image analysis (option)
CO2 and O2 control (option)	0 – 20% CO2 control and 1 – 19% O2 control, with optional Gas Controller
Environmental control cover	Top cover for light tight imaging and incubation control (option)
X/Y Stage resolution	Lead screw driven stage with 0.1 micron resolution	Lead screw driven stage with 0.1 micron resolution
Humidity control	Humidity chamber with rapid gas recharge (option)
Physical Characteristics
Power	250 Watts max.	250 Watts max.
Dimensions	With cover closed or without cover: 18.3″ D, 17.9″ W, 14.1″ H (46.5 cm x 45.5 cm x 35.8 cm)	18.3″ D, 17.9″ W, 14.1″ H (46.5 cm x 45.5 cm x 35.8 cm)

	With cover fully open: 18.3″ D, 17.9″ W, 27.5″ H (46.5 cm x 45.5 cm x 69.8 cm)
Weight	Without environmental control cover: 51 lbs (23.1 kg)	51 lbs (23.1 kg)

	With environmental control cover: 58 lbs (26.3 kg)
Regulatory
Regulatory	CE and TUV marked. RoHS compliant. Models for In Vitro Diagnostic use are available.	CE and TUV marked. RoHS compliant. Models for In Vitro Diagnostic use are available.
Imaging System
Imaging mode	Fluorescence, brightfield, color brightfield, and phase contrast	Fluorescence, high contrast brightfield and color brightfield
Imaging method	Single color, multi-color, montage, time lapse, Z-stacking, burst mode	Single color, multi-color, montage, time lapse, Z-stack, z-stack montage, burst mode
Light source	High power LEDs (available wavelengths: 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm)	High power LEDs (available wavelengths: 365 nm, 390 nm, 465 nm, 505 nm, 523 nm,
		590 nm, 623 nm, 655 nm, 740 nm)
Camera	16-bit gray scale, Sony CCD, 1.25 megapixel	16-bit CMOS camera. 2.3 MP Sony IMX249 1/1.2″ sensor with 5.86 micron pixel size
Camera binning	Optional 2×2 binning for focus and/or image capture	Optional 2×2 binning for focus and/or image capture
Camera exposure range	5 milliseconds to 4 seconds	5 milliseconds to 4 seconds
Image outputs available	Raw Images: 16-bit TIFF	Raw Images: 16-bit TIFF

	Saved Images: TIF, JPG, BMP, PNG, EMF, GIF	Saved Images: TIFF, JPG, BMP, PNG, EMF, GIF, color TIFF

	Movies: MP4, WMV	Movies: MP4, WMV
Objective capacity	6 onboard, user-replaceable objectives	6 onboard, user-replaceable objectives
Objectives available	Fluorescence:	Air: 1.25x, NA .04; 2.5x (2.25x eff), NA: .07; 2.5x (2.75x eff), NA: .12; 4x, NA: .13; 10x, NA: .30; 20x, NA: .45; 40x, NA.60; 60x, NA .70

	Air: 1.25x, NA .04; 2.5x (2.25x eff), NA: .07; 2.5x (2.75x eff), NA: .12; 4x, NA: .13; 10x, NA: .30;	Oil: 60x, NA:1.42; 60x, NA 1.25; 100x, NA 1.4; 100x, NA 1.3

	20x, NA: .45; 40x, NA.60; 60x, NA .70	High NA: 20x, NA 0.75; 40x, NA 0.95

	Oil: 60x, NA:1.42; 100x, NA: 1.40

	Phase objectives available: 4x, NA: .13; 10x, NA: .30; 20x, NA: .45; 40x, NA .60
Image filter cube capacity	4 user-replaceable fluorescence cubes plus brightfield channel	4 user-replaceable fluorescence cubes plus brightfield channel
Imaging filter cubes available	DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7, Acridine Orange, CFP-YFP FRET, Chlorophyll, Phycoerythrin (PE), Propidium Iodide, CY5.5, TagBFP, GFP (Ex)-CY6 (Em), RFP (Ex)-CY5 (Em), Alexa 568, Ex377 / Em647	DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7, Acridine Orange, CFP-YFP FRET, Chlorophyll, Phycoerythrin (PE), Propidium Iodide, CY5.5, TagBFP, GFP (Ex)-CY6 (Em), RFP (Ex)-CY5 (Em), Alexa 568, Ex377 / Em647
Automated functions	Autofocus, user-trained autofocus, auto exposure, auto-LED intensity	Autofocus, user-trained autofocus, auto exposure, auto-LED intensity
Autofocus method	Image-based autofocus	Image-based autofocus

	Laser autofocus option	Laser autofocus option
Image collection rate	Single well fastest frame rate capture:	Single well fastest frame rate capture:

	*Full Resolution: up to 10 frames per second for single color images	*Full Resolution: up to 10 frames per second for single color images

	*2×2 Binning: up to 20 frames per second for single color images	*2×2 Binning: up to 20 frames per second for single color images
Microscope stage control	Gen5 Software Control	Gen5 Software Control

	Optional joystick controller	Optional joystick controller
Reagent Injectors (option)
Number	2 syringe pumps
Supported labware	6- to 384-well microplates, Petri and cell culture dishes, chamber slides
Dead volume	<1.65 mL with back flush
Dispense tip options	Aligned tip – aligned with optical path for dispensing for fast kinetic assays

	Offset tip – dispensing is offset from the optical path
Dispense volume	5 – 1000 µL in 1 µL increments
Dispense accuracy	+1 µL or 2%
Dispense precision	<2% at 50 – 200 µL
Specifications are subject to change.

Specifications

GENERAL
Microplate types	6- to 1536-well plates
Other labware supported	Microscope slides, Petri and cell culture dishes, cell culture flasks (T25, T75), counting chambers (hemocytometers), chamber slides
Other labware supported	Support for labware up to 1.5″ tall
Temperature control	Incubation to 40 °C with optional environmental control cover
Software	Gen5 Microplate Reader and Imager Software included
Software	Gen5 Image and Image+ Software available for advanced image analysis (option)
CO2 and O2 control (option)	0 – 20% CO2 control and 1 – 19% O2 control, with optional Gas Controller
Environmental control cover	Top cover for light tight imaging and incubation control (option)
X/Y Stage resolution	Lead screw driven stage with 0.1 micron resolution
Humidity control	Humidity chamber with rapid gas recharge (option)
IMAGING SYSTEM
Imaging mode	Fluorescence, brightfield, color brightfield, and phase contrast
Imaging method	Single color, multi-color, montage, time lapse, Z-stacking, burst mode
Light source	High power LEDs (available wavelengths: 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm)
Camera	16-bit gray scale, Sony CCD, 1.25 megapixel
Camera binning	Optional 2×2 binning for focus and/or image capture
Camera exposure range	5 milliseconds to 4 seconds
Image outputs available	Raw Images: 16-bit TIFF
	Saved Images: TIF, JPG, BMP, PNG, EMF, GIF
	Movies: MP4, WMV
Objective capacity	6 onboard, user-replaceable objectives
Objectives available	Fluorescence:
	Air: 1.25x, NA .04; 2.5x (2.25x eff), NA: .07; 2.5x (2.75x eff), NA: .12; 4x, NA: .13; 10x, NA: .30;
	20x, NA: .45; 40x, NA.60; 60x, NA .70
	Oil: 60x, NA:1.42; 100x, NA: 1.40
	Phase objectives available: 4x, NA: .13; 10x, NA: .30; 20x, NA: .45; 40x, NA .60
Image filter cube capacity	4 user-replaceable fluorescence cubes plus brightfield channel
Imaging filter cubes available	DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7, Acridine Orange, CFP-YFP FRET, Chlorophyll, Phycoerythrin (PE), Propidium Iodide, CY5.5, TagBFP, GFP (Ex)-CY6 (Em), RFP (Ex)-CY5 (Em), Alexa 568, Ex377 / Em647
Automated functions	Autofocus, user-trained autofocus, auto exposure, auto-LED intensity
Autofocus method	Image-based autofocus
Autofocus method	Laser autofocus option
Image collection rate	Single well fastest frame rate capture:
	*Full Resolution: up to 10 frames per second for single color images
	*2×2 Binning: up to 20 frames per second for single color images
Microscope stage control	Gen5 Software Control
Microscope stage control	Optional joystick controller
REAGENT INJECTORS (OPTION)
Number	2 syringe pumps
Supported labware	6- to 384-well microplates, Petri and cell culture dishes, chamber slides
Dead volume	<1.65 mL with back flush
Dispense tip options	Aligned tip – aligned with optical path for dispensing for fast kinetic assays
Dispense tip options	Offset tip – dispensing is offset from the optical path
Dispense volume	5 – 1000 µL in 1 µL increments
Dispense accuracy	+1 µL or 2%
Dispense precision	<2% at 50 – 200 µL
PHYSICAL CHARACTERISTICS
Power	250 Watts max.
Dimensions	With cover closed or without cover: 18.3″ D, 17.9″ W, 14.1″ H (46.5 cm x 45.5 cm x 35.8 cm)
Dimensions	With cover fully open: 18.3″ D, 17.9″ W, 27.5″ H (46.5 cm x 45.5 cm x 69.8 cm)
Weight	Without environmental control cover: 51 lbs (23.1 kg)
Weight	With environmental control cover: 58 lbs (26.3 kg)
REGULATORY
Regulatory	CE and TUV marked. RoHS compliant. Models for In Vitro Diagnostic use are available.

Features

Automated digital microscopy to 100x.
Lionheart FX with Augmented Microscopy automates make your imaging workflow to yield amazing images and powerful data. Automated image capture starts with image-based and laser autofocus, plus auto LED and exposure. Automated image pre-processing optimizes images for downstream analysis, from cell counting to characterization of subcellular details.

Four imaging modes, multiple imaging processes
With brightfield, color brightfield, phase contrast and fluorescence imaging in four channels – and nearly twenty available filter/LED cubes, Lionheart FX offers fantastic versatility that isn’t limited to image capture. Follow your imaging processes through z-stacking to z-projection, montage collection to stitching and digital phase contrast to analysis, all one hardware and software platform.

Label-free cell counting
It’s fast and simple to count live cells using the available high contrast brightfield option. Higher contrast allows a direct and label-free method for object identification using Gen5 software to count cells and characterize cell proliferation in kinetic studies.

Kinetic live cell assay support
Several options are available to support live cell assays with Lionheart FX, including an environmental cover to maintain temperature and gas levels within the system. Long-term live cell kinetics are supported, using the unique humidity chamber that fits on the stage itself, keeping the live cell in a completely nurturing environment. A dual reagent injector provides quick inject/image capability for observing fast reactions.

Fully integrated and compact
Fifteen minutes is all it takes to install and set up Lionheart FX for image capture and analysis. The filter/LED cubes and objectives are accessed from the front panel, and tool-free connections for injectors, gas control and the environmental cover add to the easy installation. Its small size takes up minimal benchtop space.
Configurations

LFX
Lionheart FX Automated Live Cell Imager. Fluorescence, brightfield, color brightfield and phase contrast imaging capabililty. Includes base Gen5 software. Objectives, filter/LED cubes, accessories and software upgrades sold separately.

Brochures

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Application notes

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