The DigestPro™ is a dedicated protein digestion and sample preparation system for MS based proteomics. It completely automates all essential steps of protein digestion protocols and easily works with both in-solution and in-gel based methods.
In addition sample clean-up and MALDI spotting can be automated. It achieves high throughput with up to 96 samples at a time. The whole procedure is performed under controlled and reproducible conditions that is essential for protein analysis by mass spectrometry.
The DigestPro utilizes a unique 2-channel needle which can deliver both liquid and air. For vessel draining the needle seals on the vessel membrane and drain liquid reagents with air purging from the outer channel. Liquid delivery is accomplished through the inner channel after the needle is unsealed from the membrane. In this way liquid transfer both in and out of the vessel is robust and accurate for small volume transfers without loss of gel slices.
1. Air Purging
2. Sealing Membrane
3. Gel Slices
4. Addition of new buffer – Liquid Delivery
The DigestPro allows for automated use of ZipTips or similar reversed phase tips for easy clean-up and enrichment of samples (ZipTips are registered by EMD Millipore). Tips are stored in a rack and are picked up and deposited onto the needle for repeated access. ZipTips enable peptide binding onto a C-18 substrate that allows for washing on the tip. The peptide solution can then be eluted with a small concentrated volume of solution. Optionally, samples can directly be spotted onto MALDI targets with the tips. As an alternative to ZipTips, selfmade StageTips can be used with the DigestPro.
The DigestPro allows for rapid enzymatic digestion using programmable temperatures from 8 – 65°C. More rapid and complete digestions can be achieved with optimized elevated temperature protocols. Additionally, elevated temperature protocols can be used to simplify reduction of disulfide bonds.
The DigestPro allows for full customizability of the work area. The customer can choose different modules required for his individual applications.
Modules are available for:
Furthermore, the flexibility includes the use of different vial formats for digestion and collection, programmable temperatures from 8 – 70°C, the usage of ZipTips or StageTips, and many available reagent positions.