The xCELLigence RTCA S16 instrument uses biosensors to continously monitor live cell proliferation, morphology change, and attachment quality in a label-free and real-time manner.
The instrument operates in a standard CO2 cell culture incubator and the control unit is housed outside the incubator. User friendly software allows for real-time control and monitoring of the instrument with real-time data analysis functions.
|Analyzer Application||Adhesion||Apoptosis||Cell Characterization|
|Cytotoxity||Immune Cell Killing||Proliferation|
|Receptor Signaling||Stem Cells||Virus Cytopathic Effects|
|Operating Environment Relative Humidity||5-98 %|
|Operating Environment Temperature||20-40 °C|
|Sampling Format||16-well plate|
How is xCELLigence RTCA used for killing assays? The xCELLigence Real-Time Cell Analysis (RTCA) instrument monitors cell killing in real time. Simply add target and immune effector cells to the patented microtiter plates (E-Plates), load the instrument, and start reading. The biosensors embedded in the bottom of each well allow for the automated kinetic measurement of changes in cell number, size, and substrate attachment quality.
Target cell death can be measured continuously and automatically from hours to days without the use of labels.
Cellular Impedance Explained
Positioned between reductionistic biochemical assays and whole organism in vivo experimentation, cell-based assays serve as an indispensable tool for basic and applied biological research. However, the utility of many cell-based assays is diminished by: (1) the need to use labels, (2) incompatibility with continuous monitoring (i.e. only end point data is produced), (3) incompatibility with orthogonal assays, and (4) the inability to provide an objective/quantitative readout. Each of these shortcomings is, however, overcome by the non-invasive, label-free, and real-time cellular impedance assay.
Agilent’s cell analysers provide an easy, fast, and broad way to monitor cell health, behaviour and function in real-time. With the usage of microplates embedded with gold biosensors at the bottom of each well, the measurements are done through an electric impedance. This results in a label-free and easy workflow with excellent accuracy and reproducibility. Cell behaviour such as cell size, number, proliferation and kinetic analysis of cell invasion or migration can be measured with the xCELLigence instruments. This unique technology can be combined with live cell imaging with the eSight.
The xCELLigence RTCA S16 instrument provides a quantitative measurement of cell number, size, volume and substrate attachment quality. The instrument offers the capacity for 16 wells and measures via its biosensors, providing a continuous cell analysis. The instrument operates in a standard CO2 cell culture incubator and the control unit is housed outside the incubator. User friendly software allows for real-time control and monitoring of the instrument with real-time data analysis functions
• Cancer immunotherapy:
With the monitoring of cell killing
• Virology & Infectious diseases:
Monitor pathogens behaviour
• Cell signalling:
Capture changes in cell behaviour due to signalling
• Cytotoxicity Overview:
Monitor cell behaviour and attachment to the plate surface
• Cell Barrier Function:
Continuous monitoring of the cell barrier
• Cell Adhesion:
Studying cell adhesion and cell spreading