The xCELLigence RTCA HT instrument uses biosensors to continuously monitor cell proliferation, morphology change, and attachment quality with a label-free protocol in a 384-well format for high throughput screening experiments, such as antibody screening. Observe the real-time effects of therapeutic antibodies on live cells.
Integrate up to 4 instruments controlled by a single control unit for 1,536 wells to meet high throughput sample screening needs. The RTCA HT model can be used as a single instrument or integrated into a multi-instrument high-throughput workflow with automated liquid handling.
|Analyzer Application||Adhesion||Apoptosis||Cell Characterization|
|Cytotoxity||Immune Cell Killing||Proliferation|
|Receptor Signaling||Stem Cells||Virus Cytopathic Effects|
|Operating Environment Relative Humidity||5-98 %|
|Operating Environment Temperature||15-32 °C|
|Sampling Format||384-well plate|
How is xCELLigence RTCA used for killing assays? The xCELLigence Real-Time Cell Analysis (RTCA) instrument monitors cell killing in real time. Simply add target and immune effector cells to the patented microtiter plates (E-Plates), load the instrument, and start reading. The biosensors embedded in the bottom of each well allow for the automated kinetic measurement of changes in cell number, size, and substrate attachment quality.
Target cell death can be measured continuously and automatically from hours to days without the use of labels.
Cellular Impedance Explained
Positioned between reductionistic biochemical assays and whole organism in vivo experimentation, cell-based assays serve as an indispensable tool for basic and applied biological research. However, the utility of many cell-based assays is diminished by: (1) the need to use labels, (2) incompatibility with continuous monitoring (i.e. only end point data is produced), (3) incompatibility with orthogonal assays, and (4) the inability to provide an objective/quantitative readout. Each of these shortcomings is, however, overcome by the non-invasive, label-free, and real-time cellular impedance assay.
The xCELLigence High Throughput (HT) model offers a quick and simple way to continuously monitor cell proliferation, morphology change and attachment quality for high throughput screening experiments. The platform offers a 384-well format. For even higher throughput demands, up to four instruments can be integrated and controlled by a single control unit. can be integrated with up to four instruments to meet high throughput screening needs
• Cancer immunotherapy:
With the monitoring of cell killing
• Virology & Infectious diseases:
Monitor pathogens behaviour
• Cell signalling:
Capture changes in cell behaviour due to signalling
• Cytotoxicity Overview:
Monitor cell behaviour and attachment to the plate surface
• Cell Barrier Function:
Continuous monitoring of the cell barrier
• Cell Adhesion:
Studying cell adhesion and cell spreading