NovoCyte Quanteon

Short product description
4 laser flow cytometer that can be configured with up to 25 independent photomultipliers


The NovoCyte Quanteon is a new generation in the successful line of NovoCyte flow cytometers. The Quanteon is a 4 laser flow cytometer that can be configured with up to 25 independent photomultipliers for meeting the most demanding sample panels. The proprietary photomultiplier technology employed in the Quanteon provides excellent sensitivity, stability, and a 7.2 log dynamic range. Very dim signals and very bright signals can all be captured and gated in the same view, therefore eliminating the need for laborious trial and error PMT tuning procedures.

When developing large color panels, the Quanteon saves hours of setup and analysis time. Excellent fluidics provide steady and consistent sample delivery, with high reproducibility and exceptionally low CVs. Absolute counts, without the use of expensive beads, will save you time and money.

  • Simple operation with fully automated wash and shut down features
  • Efficient, walk-away automation with the NovoSampler Q
  • Flexible sample handling provides efficient mixing, even at ultralow volumes, accommodates the most popular plate formats, bar coding, and built-in API’s for lab automation systems
  • Easy to use NovoExpress software with auto-compensation features, cell cycle analysis modules, cell proliferation modules, heat maps, and batch statistical analysis.
“The advantage of the new flow cytometers is that they are accompanied by user-friendly software, which makes operating the instrument relatively easy” Read the testimonial of the Flow Cytometry Unit (FCU) of the UMCG about their 2 NovoCyte Quanteon Flow Cytometers here
349 nm
405 nm
488 nm
561 nm
637 nm
Maximum Number of Fluorescence Channels
NovoCyte Advanteon** 1
NovoCyte Quanteon** 4
NovoCyte Penteon* 5
* RUO: Research use only. Not for use in diagnostic procedures.
** Selected configurations are registered as CE-IVD.

Apoptosis Assay
Apoptosis, or programmed cell death, is the process by which cells regulate how they die, activating specific pathways that cause the cell to shrink, condense, and eventually be cleared by phagocytosis. This is in contrast to necrotic cell death where cells die uncontrollably and fall apart, which can lead to detrimental effects such as the activation of an immune response. Therefore, apoptotic cells that die in a very orderly fashion limit disruption of nearby cells and tissue.
There are many ways to measure cell death and distinguish it from apoptosis or necrosis. These assays are easily quantified using the NovoCyte flow cytometer due to automatic compensation settings and a wide dynamic range of fluorescence detection which eliminates the need for any PMT voltage adjustments.

Immune status is associated with disease state, treatment efficiency, and response to external stimuli such as vaccines. Immunophenotyping quickly identifies candidate cell types, sub-classes and functions. Monitoring the frequency of numerous immune cell population as well as the differentiation/activation status of specific cell subsets such as monocytes, NK cells, T and B cells is essential as they may influence the immunogenicity of a vaccine and its efficiency. The NovoCyte Flow Cytometer enables simultaneous quantification of multiple leukocytes for better understanding the immune status of patients and surveillance of the immune response to infectious disease.

Specifcity Clone Fluorocrome Purpose
CD3 UCHT1 PE-TR (ECD) Lineage T cells
CD4 S3.6 PE-Alexa 700 Lineage T cells
CD8 SK1 PerCP-Cy5.5″ Lineage T cells
CD19 J3-129 PerCP-eFluor 710 B cells
CD14 MφP9 BV711 Monocytes
CD56 HCD56 BV605 NK cells and NK T-like cells
CD16 3G8 APC-Cy7 NK cells and monocytes
γδ TCR 11F2 PE-Cy7 γδ T cells
Vγ2 TCR B6 PE γδ T cells
CD25 M-A251 BV421 Tregs
CD127 A019D5 APC Tregs/memory/differentiation
CD45RA HI100 BV650 Memory/differentiation
CCR7 G043H7 BV785 Memory/differentiation
CD57 NK-1 FITC Memory/differentiation
HLA-DR B169414 BV570 Activation
CD38 HIT2 PE-Cy5 Activation/plasmablasts
NKG2C 134591 Alexa 700 NK receptor
Dead Cells 134591 AViD Dead cell exclusion


Intracellular Protein Detection
Detection and analysis of intracellular proteins allow for additional characterization of cell subpopulations and cellular processes. In order to analyze proteins not located on the cell surface, fixation and permeabilization of the cell is required. However, many phospho-specific antibodies are not compatible with many common detergent-based permeabilization methods used for intracellular staining. Special attention is needed when determining the proper fix/perm method for your phospho-specific antibody. The most common method uses 1.5% paraformaldehyde for fixation followed by 100% methanol for permeabilization. While this method works for many antibodies, please note it may not work for every phospho-specific antibody.

Additionally, identifying various cell populations in a heterogenous sample requires staining for phosphorylated proteins coupled with surface proteins. Special consideration must be given to the sensitivity of these epitopes to fixative, taking precaution to avoid damage to the epitope. Therefore, the sample may require staining for specific surface markers before fixation.

Cell Cycle Analysis
Normal human somatic cells are diploids containing a constant amount of DNA. During cell cycle progression, DNA synthesis results in a doubling of total DNA content, followed by restoration of the normal DNA content after mitosis. Detailed cell cycle analysis can be performed to understand tumor cell differentiation, cell transformation and cell-compound interaction with the NovoCyte flow cytometer.

Figure: After treatment with 10 migrograms/M MG132 or 500 micrograms/M 5-FI for 16 hours/ A549 cells were analyzed for cell cycle distribution with the ACEA Novocyte flow cytometer. The the Novoexpress built-in cell cycle analysis module, the plot shows cells ni G0/G1 phase (green), S phase (yellow) and G2/M phase (blue). Compared to normal untreated cells, MG132 treated cells were arrested at G2/M phase, while 5-FU treated cells were arrested at G0/G1 phase.

Cell Proliferation
Cell proliferation is an essential function and highly structured event that when unregulated, can cause disease. We can measure proliferation through absolute cell counts or with a dye, such as CFSE. When cells labeled with CFSE divide, the dye is partitioned equally between daughter cells and we can measure the loss of CFSE fluorescence over time as the dye is continuously diluted. The mean fluorescence intensity (MFI) of the dye was also plotted with cell concentration over time to show the inverse relationship between the two. This type of assay is often used to look at changes in T lymphocyte activation.

The Ultimate Photodetector
Silicon photomultipliers (SiPM) are solid-state, silicon-substrate-based, photon-level-sensitive semiconductor devices, with a 7.2 log dynamic range. Consisting of a compact array of avalanche photodiodes operating in unison, the SiPM is a compact detector with photon counting capability. The innovative optics designed into the NovoCyte Penteon incorporates 30 independent SiPM, which collect and process signals for each of its fluorescence channels.

Excellent scatter resolution to detect small particles
NovoCyte Penteon scatter detection optics and signal processing electronics have been optimized to resolve particles down to 0.1μm in size. With such excellent resolution, platelets, bacteria, and various submicron particles can be readily identified and analyzed.

High reproducibility and stability
The NovoCyte Penteon and NovoCyte Quanteon’s fluidic system is designed to deliver high performance. When compared to other flow cytometers, the fluidic consistency and stability of the NovoCyte Penteon and NovoCyte Quanteon is unmatched. Other instruments utilizing peristaltic pumps are often subject to fluidic pulsation, causing inconsistency and inaccuracy in absolute cell counts.

This 4-laser flow cytometer can be configured with up to 25 independent silicon photomultipliers (SiPM). Its operation is simple with fully automated wash and shut down features. The instrument is able to analyse the cell cycle, cell proliferation, heat maps and batch statistical analysis. Together with the easy-to-use software NovoExpress, the instrument provides a broad and easy sample analysis. The SiPM technology employed in the Quanteon result in excellent photon-level sensitivity, stability and a 7.2-log dynamic range. Very dim signals and very bright signals can all be captured and gated in the same view, therefore eliminating the need for laborious trial-and-error PMT tuning procedures.


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Flow Cytometry Automation


The Netherlands

P: +31 (0)294 760 088
E: [email protected]

Bovenkamp 9
1391 LA Abcoude


P: +32 (0)2 334 22 74
E: [email protected]

Steenweg op/Chaussée de Ruisbroek 290b
1620 Drogenbos

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