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NovoCyte Penteon

Short product description
Choose from up to 30 fluorescence channels utilizing up to 5 lasers with up to 30 independent detectors


The NovoCyte Penteon flow cytometer builds on its successful predecessor, the NovoCyte, and provides an expanded set of capabilities that accommodate today’s high-end and increasingly sophisticated multi-color flow cytometry assays. You now have the flexibility to choose from up to 30 fluorescence channels utilizing up to 5 lasers with up to 30 independent detectors.

The NovoSampler Q can be integrated into different laboratory automation platforms and efficiently processes both FACS tubes (using a 40-tube rack) and 24-, 48-, 96-, and 384-well plates. The intuitive and industry leading NovoExpress software has been further advanced, providing an exceptional user experience in data acquisition, analysis and reporting.

  • Expanded flexibility with 30-fluorescence channel options using 5 lasers
  • High sensitivity and resolution
  • Intuitive and powerful software for data acquisition, analysis, and reporting
  • Smart-design functionalities and walk-away operation to simplify your workflow
  • Automation-ready capability for high throughput needs
  • Wide, 7-log dynamic range eliminates the need for routine detector adjustments
  • Models

    349 nm
    405 nm
    488 nm
    561 nm
    637 nm
    Maximum Number of Fluorescence Channels
    NovoCyte Advanteon**1
    NovoCyte Quanteon**4
    NovoCyte Penteon*5
    * RUO: Research use only. Not for use in diagnostic procedures.
    ** Selected configurations are registered as CE-IVD.
  • Applications

    Apoptosis Assay
    Apoptosis, or programmed cell death, is the process by which cells regulate how they die, activating specific pathways that cause the cell to shrink, condense, and eventually be cleared by phagocytosis. This is in contrast to necrotic cell death where cells die uncontrollably and fall apart, which can lead to detrimental effects such as the activation of an immune response. Therefore, apoptotic cells that die in a very orderly fashion limit disruption of nearby cells and tissue.
    There are many ways to measure cell death and distinguish it from apoptosis or necrosis. These assays are easily quantified using the NovoCyte flow cytometer due to automatic compensation settings and a wide dynamic range of fluorescence detection which eliminates the need for any PMT voltage adjustments.

    Immune status is associated with disease state, treatment efficiency, and response to external stimuli such as vaccines. Immunophenotyping quickly identifies candidate cell types, sub-classes and functions. Monitoring the frequency of numerous immune cell population as well as the differentiation/activation status of specific cell subsets such as monocytes, NK cells, T and B cells is essential as they may influence the immunogenicity of a vaccine and its efficiency. The NovoCyte Flow Cytometer enables simultaneous quantification of multiple leukocytes for better understanding the immune status of patients and surveillance of the immune response to infectious disease.

    CD3UCHT1PE-TR (ECD)Lineage T cells
    CD4S3.6PE-Alexa 700Lineage T cells
    CD8SK1PerCP-Cy5.5″Lineage T cells
    CD19J3-129PerCP-eFluor 710B cells
    CD56HCD56BV605NK cells and NK T-like cells
    CD163G8APC-Cy7NK cells and monocytes
    γδ TCR11F2PE-Cy7γδ T cells
    Vγ2 TCRB6PEγδ T cells
    NKG2C134591Alexa 700NK receptor
    Dead Cells134591AViDDead cell exclusion


    Intracellular Protein Detection
    Detection and analysis of intracellular proteins allow for additional characterization of cell subpopulations and cellular processes. In order to analyze proteins not located on the cell surface, fixation and permeabilization of the cell is required. However, many phospho-specific antibodies are not compatible with many common detergent-based permeabilization methods used for intracellular staining. Special attention is needed when determining the proper fix/perm method for your phospho-specific antibody. The most common method uses 1.5% paraformaldehyde for fixation followed by 100% methanol for permeabilization. While this method works for many antibodies, please note it may not work for every phospho-specific antibody.

    Additionally, identifying various cell populations in a heterogenous sample requires staining for phosphorylated proteins coupled with surface proteins. Special consideration must be given to the sensitivity of these epitopes to fixative, taking precaution to avoid damage to the epitope. Therefore, the sample may require staining for specific surface markers before fixation.

    Cell Cycle Analysis
    Normal human somatic cells are diploids containing a constant amount of DNA. During cell cycle progression, DNA synthesis results in a doubling of total DNA content, followed by restoration of the normal DNA content after mitosis. Detailed cell cycle analysis can be performed to understand tumor cell differentiation, cell transformation and cell-compound interaction with the NovoCyte flow cytometer.


    Cell Proliferation
    Cell proliferation is an essential function and highly structured event that when unregulated, can cause disease. We can measure proliferation through absolute cell counts or with a dye, such as CFSE. When cells labeled with CFSE divide, the dye is partitioned equally between daughter cells and we can measure the loss of CFSE fluorescence over time as the dye is continuously diluted. The mean fluorescence intensity (MFI) of the dye was also plotted with cell concentration over time to show the inverse relationship between the two. This type of assay is often used to look at changes in T lymphocyte activation.

  • How it works

    The Ultimate Photodetector
    Silicon photomultipliers (SiPM) are solid-state, silicon-substrate-based, photon-level-sensitive semiconductor devices, with a 7.2 log dynamic range. Consisting of a compact array of avalanche photodiodes operating in unison, the SiPM is a compact detector with photon counting capability. The innovative optics designed into the NovoCyte Penteon incorporates 30 independent SiPM, which collect and process signals for each of its fluorescence channels.

    Excellent scatter resolution to detect small particles
    NovoCyte Penteon scatter detection optics and signal processing electronics have been optimized to resolve particles down to 0.1μm in size. With such excellent resolution, platelets, bacteria, and various submicron particles can be readily identified and analyzed.

    High reproducibility and stability
    The NovoCyte Penteon and NovoCyte Quanteon’s fluidic system is designed to deliver high performance. When compared to other flow cytometers, the fluidic consistency and stability of the NovoCyte Penteon and NovoCyte Quanteon is unmatched. Other instruments utilizing peristaltic pumps are often subject to fluidic pulsation, causing inconsistency and inaccuracy in absolute cell counts.

  • Feaures

    The NovoCyte Penteon is able to provide multi-coloured flow cytometry assays with the flexibility to choose from 30 fluorescence channels. The instrument utilizes 5 lasers (including UV-laser) and 30 independent detectors.

    The high sensitivity and resolution instrument is able to operate with FACS tubes and 24-, 48-, 96-, 384-well plates. For the NovoCyte Penteon, the intuitive NovoExpress software is now even more advanced, providing an exceptional user experience in data acquisition, analysis, and reporting. When developing large colour panels, the Quanteon saves hours of setup and analysis time. Excellent fluidics provide steady and consistent sample delivery, with high reproducibility and exceptionally low CVs. Absolute counts, without the use of expensive beads, will save you time and money.


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Application notes

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Flow Cytometry Automation