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BioTek EL406
Information
The EL406 Combination Washer Dispenser offers fast, full plate washing along with three reagent dispensers in one, compact instrument. Its multiple capabilities include washing and dispensing for standard assays, plus biomagnetic separation and vacuum filtration protocols. The patented Dual-Action™ manifold optimizes washing for loosely adherent cell monolayers, while the patented Ultrasonic Advantage™ helps prevent blockages in the manifold tubes before washing, to reduce assay failure.
Syringe pump and peristaltic pump dispensers each offer unique advantages, and the EL406 offers both, along with superior plate washing, on a single platform – making the EL406 a workhorse for your laboratory.
- Combines fast microplate washing with 3 reagent dispensers in 1 compact instrument
- 96-, 384- and 1536-well plate compatible
- Gentle processing for cell-based assays
- Bead washing: magnetic and polystyrene
- Easy maintenance with Ultrasonic Advantage™
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Configurations
Part # 1536-/ 384-/ 96-well 1536-/ 384-well 1536-well 384-/ 96-well 384-well 96-well 3 reagent disp. 2 reagent disp. 1 reagent disp. 406PSUB3SB • • 406PSUB3LB • • 406PSUB1SB • • 406PSUB1LB • • 406PSUB2SB • • 406PSUB2LB • • 406PSU0SB • • 406PSU0LB • • 406SNA0LB • • 406PSUB3 • • 406PSUB1 • • 406PUB3 • • 406PUB1 • • 406PSUB2 • • 406PUB2 • • 406PSNA4 • • 406SNA4 • • 406P4 • • EL406™ 1536-/384-/96-well Microplate Washer Dispenser Configurations Feature Design P 1-Reagent Dispense Module – Autoclavable 1 Peristaltic Pump 8-tip (1 x 8) cassette Sapphire jeweled, steel or plastic tips S 2-Reagent Dispense Module – Autoclavable 2 Syringe Pumps 2 16-tube (1 x 16) manifolds Steel tubes SNA 2-Reagent Dispense Module – Non-autoclavable 2 Syringe Pumps 1 16-tube (2 x 8) manifolds – except with P/N 406SNA0-LB Steel tubes U Wash Module – Ultrasonic Advantage™ B Wash Module – Automatic 4-Buffer Switching 1 Wash Module – 384-/96-well microplate washing Dual-Action™ 96-tube (8 x 12) manifold Steel tubes 2 Wash Module – faster 384-well microplate washing Dual-Action™ 192-tube (16 x 12) manifold Steel tubes 3 Wash Module – 384-/96-well microplate washing Dual-Action™ Interchangeable 96-tube (384-/96- well washing) and 192-tube (faster 384-well washing) manifolds Steel tubes 4 Wash Module – 96-well microplate washing 96-tube (8 x 12) manifold Steel tubes SB Wash Module – 1536-well microplate washing and dispensing 2 32-tube (1 x 32) dispense manifolds Small bore, sapphire jeweled tubes 128-tube (4 x 32) aspiration manifold LB Wash Module – 1536-well microplate washing and dispensing 2 32-tube (1 x 32) dispense manifolds Large bore, steel tubes 128-tube (4 x 32) aspiration manifold -
specifications
GENERAL Microplate types Dispensing (peri pump and syringe) 96-, 384- and 1536-well standard, deep and PCR plates; 6-. 12-, 24- and 48-well plates (dispense tip configurable) Washing 96-. 384-well standard plates; 6-, 12-, 24-,and 48-well plates (with compatible manifold configuration) Onboard software Create, edit or run multiple protocols Software (computer control) LHC2 Software LHC2 Secure for 21 CFR Part 11 compliance (option) SiLA Compliant driver (option) Shaking Programmable up to 60 minutes Slow, medium, fast or variable Soaking Programmable up to 60 minutes USB ports (2) For protocol storage/transfer and for optional external mouse or keyboard Automation BioStack and 3rd party automation compatible BioSpa 8 Automated Incubator compatible WASHING Manifold types 96- and 384-well washing: 8-tube manifold 6-, 12-, 24-, 48-well washing: Custom manifolds available Volume range 20-30,000 µL/well Wash cycles 1-okt Supply bottle 2 L Dispense accuracy 3% Dispense precision 96-/384-well plates, 300 µL/well: <3% CV 6-well plates, 5560 µL/well: <5% CV Residual volume 96-well plate, 300 µL/well: < 2 µL/well Wash speed 96-wells, 8-tube manifold, 3 cycles, >300 µL/well: <130 seconds Wash volume 20-30,000 µL/well Waste bottle 2L Flow rates 140-422 µL/well/second Sterilization Chemical DISPENSING – PERISTALTIC PUMP (MULTI-CHANNEL) Manifold types 1 x 8 – sapphire jeweled 316 SS, 316 SS or polypropylene tips, with 1, 5 or 10 µL tubing Fluid delivery 1 or 2 peristaltic pumps Dispense speed 96 wells, 5 µL cass, 10 µL/ well: 3 seconds 96 wells, 5 µL cass, 20 µL/ well: 3.5 seconds 384 wells, 5 µL cass, 5 µL/ well: 6.5 seconds 384 wells, 10 µL cass, 10 µL/ well: 8 seconds 384 wells, 1 µL cass, 1 µL/ well: 6 seconds 1536 wells, 1 µL cass, 1 µL/ well: 21 seconds Volume range 500 nL – 3,000 µL/well, selectable in 1 µL increments Flow rates User programmable rates from high to low Dispense performance 1 µL cassette: recommended range: 1 – 50 µL Accuracy: +5% at 1 µL Precision: <5% CV at 1 µL <10% CV at 500 nL Minimum Prime Volume: 1.20 mL 5 µL cassette: recommended range: 5 – 2,500 µL Accuracy: +2.0% at 5 µL Precision: <2.5% CV at 5 µL Minimum Prime Volume: 4.23 mL 10 µL cassette: recommended range: 10 – 3,000 µL Dispense Accuracy: +2.0% at 10 µL Dispense Precision: <2.0% CV at 10 µL Minimum Prime Volume: 7.36 mL Recommended cassette replacement interval 1 µL Cassette: 1,000 384-well microplates at 5 µL well 5 µL Cassette: 1,000 96-well microplates at 50 µL well 10 µL Cassette: 1,000 96-well microplates at 100 µL well Sterilization Autoclave, chemical DISPENSING – SYRINGE PUMP Manifold types 96- and 384-well dispensing: One 16-tube (2 x 8) manifolds – 316 SS tubes Two 16-tube (1 x 16) manifolds – 316 SS tubes 1536-well dispensing: Two 32-tube (1 x 32) manifolds – sapphire jeweled 316 SS or 316 SS tubes 6- to 48-well dispensing: custom autoclavable manifolds available Fluid delivery Two positive displacement syringe drives Dispense speed 20 µL /well, 96 wells, 1 x16 tubes:: 5 seconds 20 µL /well, 384 wells, 1 x16 tubes:: 14 seconds 3 µL /well, 1536 wells, 2 x 32 tubes:: 7 seconds Volume range 3 – 3,000 µL/well selectable in 1 µL increments Minimum Prime Volume: 12 mL Flow rates User programmable rates from high to low Dispense accuracy +1 µL at 5 µL +1 µL at 20 µL +1% at 100 µL Dispense precision <5% CV at 5 µL <2.5% CV at 20 µL <1% CV at 100 µL Supply bottle 1 L or 2 L Sterilization Chemical, autoclavable option DISPENSING – PERISTALTIC PUMP (RAD TECHNOLOGY) Labware types Single tip: 6-, 12-, 24-, 48-, 96-, 384-well plates; low profile standard height and deep well formats; PCR trays and microtubes 8-to-1 tip: 6-, 12-, and 24-well plates Manifold Types RAD single, with plastic or steel tip with 1, 5 or 10 tubing, 7° angle RAD 8-to-1 plastic tip, with 5 µL tubing, angled bulk dispense chute Volume range 500 nL – 30,000 µL Minimum prime volume 1 µL cass, 18″: 90 µL ; 1 µL cass, 30″: 150 µL 5 µL cass, 18″: 320 µL; 1 µL cass, 30″: 530 µL 10 µL cass, 18″: 555 µL; 10 µL cass, 30″: 920 µL Dispense speed (high flow) 1 µL cass, 1 µL/well: 19s (96 wells) 55s (384 wells) 1 µL cass, 10 µL/well: 33s (96 wells), 112s (384 wells) 5 µL cass, 5 µL/well: 19s (96 wells), 58s (384 wells) 5 µL cass, 100 µL/well: 76s (96 wells), 286s (384 wells) 10 µL cass, 10 µL/well: 21s (96 wells), 66s (384 wells) 10 µL cass, 100 µL/well: 70s (96 wells), 259s (384 wells) Dispense performance 1 µL cass (med), 0.5 µL/well: Precision 10% CV 1 µL cass (med), 1 µL/well: Accuracy + 10%, Precision 10% CV 1 µL cass (med), >2 µL/well: Accuracy + 5%, Precision 5% CV 5 µL cass (high), 5 µL/well: Accuracy + 4%, Precision 5% CV 5 µL cass (high), >10 µL/well: Accuracy + 2%, Precision 2.5% CV 10 µL cass (high), 10 µL/well: Accuracy + 4%,Precision 4% CV 10 µL cass (high), >20 µL/well: Accuracy + 2%, Precision 2 %CV 8-to 1 cass(high), 40 µL/well: Accuracy + 4% 8-to 1 cass (high), >10 µL/well: Precision 2.5%CV 8- to 1cass(high), >80 µL/well: Accuracy + 2% PHYSICAL CHARACTERISTICS Power 100 – 240 Volts AC. 50/60 Hz Dimensions Base instrument: 17.19″W x 11.75″ D x 8″ H (43.51 x 29.21 x 20.32 cm) Weight Base instrument: 19.5 lbs (8.8 Kg) REGULATORY Regulatory CE and TUV marked, RoHS Compliant. Models for In Vitro Diagnostic use are available. -
Software
The EL406 has extensive onboard software, with an intuitive menu-driven interface. Program all aspects of the system’s dispense, wash and maintenance parameters, quickly and easily. Up to 75 programs can be stored onboard.
LHC
LHC Software allows computer-based programming of multi-step processes and it is used for integrating BioTek’s multi-mode washer dispensers, washers and dispensers into robotic systems. You can operate the system under computer control via LHC, or create protocols in LHC and download to the instrument for recall and execution.LHC Secure software
LHC Secure software offers features to facilitate compliance to FDA directive 21 CFR Part 11
with computer control of BioTek’s Multi-Mode Washer Dispensers, Washers and Dispensers.LHC SiLA software
LHC SiLA softwaredriver enables BioTek washer and dispenser integration into SiLA compliant automated systems. Includes LHC software for computer control and third party integration of BioTek’s Multi-Mode Washer Dispensers, Washers and Dispensers. -
Features
Combines fast microplate washing with 3 reagent dispensers in 1 compact instrument
There’s no need for multiple instruments when you have an EL406 in the lab! The EL406 is microplate washer with peristaltic and syringe pump modules available to make it into an efficient multi-tasking system. For benchtop use or integrated into automated systems, the EL406 does the work of multiple instruments.96-, 384- and 1536-well plate compatible
BioTek’s patented Dual-Action™ manifold quickly washes 96- and 384-well plates. For 1536 washing, unique aspirate and dispense manifolds rapidly dispense and aspirate across the plate. Wash speeds for the three plate types range from 13 seconds to 36 seconds, keeping all your processes efficient.Gentle processing for cell-based assays
Cell-based assays can be challenging to wash – too often, cell monolayers are disrupted by vigorous dispensing or overly intense aspiration. The EL406 Dual-Action manifold and plate carrier have x-y-z adjustments, and flow rates are fully adjustable – to optimize parameters for even the most sensitive assays. Angled dispense tubes direct fluid flow to the well sides, further minimizing disruption.Bead washing: magnetic and polystyrene
The EL406 is a powerhouse for many standard plate washing and dispensing protocols, but it also automates more complex operations like magnetic and polystyrene bead washing workflows – and it’s Luminex® xMap® approved. Bead washing is available for 96- and 384-well microplates.Easy maintenance with Ultrasonic Advantage™
EL406 has a number of automated system maintenance features, including. BioTek’s patented Ultrasonic Advantage™. The dispense and aspirate tubes are immersed into the built-in ultrasonic bath in the priming trough while the automate cleaning program runs, dislodging and aspirating away salt crystal and protein buildup in the tubes.
Brochures
Application notes
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