Agilent xCELLigence RTCA eSight - Imaging & Impedance

RTCA Analyzer

Overview

The xCELLigence RTCA eSight enables comprehensive insight into cell health, cell behavior, cell function and cell biology processes using live, simultaneous, and real time biosensor impedance-based and image-based measurements. The eSight system combines the label-free xCELLigence RTCA technology with live cell imaging in 3 colors (red, green, and blue). This combination allows for informative live cell analysis and increased insight into cell health.

Combining microscopy with impedance?
check out the Agilent xCELLigence RTCA MultiPlaye.

• Combine label-free xCELLigence real time cell analysis technology with live cell imaging in red, green, and blue for increased confidence in your cell analysis and conclusions.
• Simple workflow adapts to differing experimental protocols to deliver physiologically relevant data while monitoring cell health, strength of adhesion, changes in morphology, proliferation, and cytolysis in primary cell cultures or standard tissue culture cell lines.
• Flexible live cell imaging utilizes brightfield capabilities, three fluorescence channels, a multitude of well plate formats, and the capability of user-defined schedules.



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Agilent xCELLigence RTCA eSight - Imaging & Impedancet



The eSight offers a unique combination of the features of xCELLigence with live cell imaging. Even more information can be gathered from the live cell analysis when using impedance-based biosensors and image-based measurements together. Both quantitative and qualitative phenotypic information about cell health, behaviour, function and biology processes can be gathered through these combined technologies. The eSight images in 3 colours: red, green, and blue to provide powerful and informative images of cells.

Typical Applications
• Cancer immunotherapy:
With the monitoring of cell killing

• Virology & Infectious diseases:
Monitor pathogens behaviour

• Cell signalling:
Capture changes in cell behaviour due to signalling

• Cytotoxicity Overview:
Monitor cell behaviour and attachment to the plate surface

• Cell Barrier Function:
Continuous monitoring of the cell barrier

• Cell Adhesion:
Studying cell adhesion and cell spreading

Single Setup for Dual Measurements
Live cell imaging and real-time biosensor measurements can be performed on the same cell populations, which provides insightful information on cell behavior. The streamlined workflow, high reproducibility, and quantitative kinetics of the eSight system makes it ideal for a wide range of cell-based assays such as proliferation, cytotoxicity, and apoptosis.

The powerful RTCA software integrates two data types into one temporal display. Cell analysis can be displayed and exported into diverse formats, which include RTCA images, KT50 (time to reach 50% cytolysis at a given E:T ratio), % Cytolysis dose response, or IC50 dose response curves.


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Multi-modal Data Acquisition
In concert with the biosensor measurements, cell images can be captured in real-time, thereby providing a spatial and temporally dynamic view of cell populations, which can further validate time-dependent cell health and behavior at an unprecedented level of detail for any cell-based assay.


Patented Real Time Cell Analysis
The impedance-based xCELLigence technology utilizes proprietary microplates (E-Plates) embedded with gold biosensors at the bottom of each well, which serve to non-invasively quantify cell behavior. Over the course of an experiment, the biosensors monitor cell metrics such as proliferation, adhesion strength, changes in morphology, migration, differentiation, and much more. The impedance measurements are exceptionally fast and provide exquisite temporal resolution so that all relevant responses can be measured in seconds, minutes, hours, or days.

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Step 1 Seed cells, then place plates or tissue culture vessels on the eSight in the incubator. Set up data and imaging acquisition schedule using eSight software.

workflow



Step 2
Add treatments (e.g. immune effector cells, antibodies, anti-viral drugs)
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Step 3
Automatically acquire data with the proprietary xCELLigence biosensor technology and overlay images in real time
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Apoptosis Assays
The process of apoptosis encompasses a number of morphological, structural, and molecular events culminating in cell demise. The xCELLigence RTCA eSight provides multiple temporal vantage points (impedance, fluorescence imaging, and brightfield imaging) from which apoptosis can be evaluated.
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Immune-cell killing assays
The xCELLigence RTCA eSight provides a valuable real-time assay to evaluate the interaction of immune cells with tumor cells in vitro, providing additional information about tumor escape as well as immune cell exhaustion.



Virus-mediated Cytopathogenicity Assays
The xCELLigence RTCA eSight instrument enables quantitative real-time assessment of viral infectivity and cytopathogenicity both at the phenotypic and mechanistic levels. Elucidate the mechanistic details of viral entry, activation of host cell anti-viral pathways, apoptotic death, and syncytium formation using impedance, fluorescence and brightfield imaging.
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Analyzer Application Adhesion Apoptosis Cell Characterization
Cytotoxity Immune Cell Killing Proliferation
Receptor Signaling Stem Cells Virus Cytopathic Effects
 Live Cell Imaging
Depth 44.5 cm
Height 41.4 cm
Operating Environment Relative Humidity 5-98 %
Operating Environment Temperature 20-40 °C
Sampling Format 3 x 96-well plate 5 x 96 well plates
Width 43.1 cm