BioTek Synergy LX

Microplate Multi-Mode Reader

Overview

Synergy™ LX Multi-Mode Microplate Reader economically automates many common microplate assays. Its large touchscreen user interface and onboard software simplify programming and operation for microplate and micro-volume assays. The high quality optic design ensures excellent data in absorbance, fluorescence and luminescence detection modes, enabling many common assays including nucleic acid and protein quantification, ELISA, BCA, Bradford and cell viability. Immediate data display, plus output to a USB flash drive, printer or Gen5™ Software makes the Synergy LX a versatile assay workstation. The upgradable design allows your lab to buy what is needed today and add other detection modes in the future.

• Affordable multi-mode reader
• Supports common endpoint assays
• Micro-volume quantification with Take3 plates
• Continuous UV-Vis wavelength selection: 200 nm to 999 nm
• High performance, high blocking filters for fluorescence and luminescence
• Touchscreen: Easy operation, immediate data display
• Output to USB flash drive, printer or Gen5 Software

Synergy LX product video.

BioTek Synergy LX Details



Affordable multi-mode reader
At half the price of similar instruments, Synergy LX is the solution for labs looking for an easy to use microplate reader that offers uncompromised performance for UV-Vis absorbance, fluorescence and luminescence assays.

Supports common endpoint assays
Synergy LX, can run the most common assays including:
• A260, A260/A280
• Protein quantification
• Fluorescence, luminescence
• ELISA
• Gene expression
• Cell viability

T Micro-volume quantification with Take3 plates
Micro-volume analysis with BioTek’s Take3 plate and Synergy LX is fast and easy, too. Pre-programmed nucleic acid and protein protocols in Synergy LX display immediate results for up to sixteen, 2 µL samples. Output results to a USB flash drive for use in downstream workflows.

Continuous UV-Vis wavelength selection: 200 nm to 999 nm
The broad, filter-free wavelength range for absorbance measurements enables many common colorimetric and UV applications, from nucleic acid and protein quantitification to turbidity measurements and more.

High performance, high blocking filters for fluorescence and luminescence
There’s no compromise on performance in Synergy LX. The high quality filters, coupled with dichroic mirrors in the Synergy LX filter cubes efficiently block background to ensure the best sensitivity for fluorescence measurements.

Touchscreen: Easy operation, immediate data display
It takes only a few touches to select and run your assay. The data is displayed immediately after the run – a color gradient helps to quickly visualize the data range.

Output to USB flash drive, printer or Gen5 Software With one touch, export data to a USB flash drive for analysis in BioTek’s Gen5 software or a spreadsheet application.
GENERAL
Detection modes UV-Vis absorbance
Fluorescence intensity
​Luminescence
Read methods Endpoint (onboard software)
Endpoint, kinetic, area scanning, absorbance spectral scanning (under Gen5 control)
Microplate types UV-Vis absorbance: 6- to 384-well plates (onboard software)
Fluorescence intensity and luminescence: 96- and 384-well (onboard software)
All modes: 6- to 384-well microplates (under Gen5 control)
Other labware supported Take3 Micro-Volume Plates (onboard software)
Take3 and Take3 Trio Micro-Volume Plates (under Gen5 control)
Shaking Linear, orbital, double-orbital
Software Endpoint protocols (onboard)
Full data analysis and reporting (under Gen5 control)
ABSORBANCE
Light source Xenon flash
Detector photodiode
Wavelength selection monochromator
Wavelength range 200 nm to 999 nm, in 1 nm increments
Monochromator bandwidth < 5 nm
Dynamic range 0 to 4.000 OD
Resolution 0.001 OD (onboard software)
0.0001 OD (under Gen5 control)
Pathlength correction yes
Monochromator wavelength accuracy + 2 nm
Monochromator wavelength repeatability + 0.2 nm (standard deviation)
OD accuracy <1% at 2.0 OD
<3% at 2.5 OD
OD linearity <1% from 0 to 2.5 OD
OD repeatability <0.5% at 2.0 OD
Stray light 0.03% at 230 nm
Reading speed (kinetic) 96 wells: 12 seconds
384 wells: 23 seconds
FLUORESCENCE INTENSITY
Light source Halogen
Detector PMT
Wavelength selection Filters
Wavelength range 320-700 nm (low noise PMT)
320-850 nm (red-shifted PMT)
Dynamic range >6 decades
Sensitivity Fluorescein 2 pM
Reading speed (kinetic) 96 wells: 24 secs
384 wells: 76 secs
LUMINESCENCE
Dynamic range >6 decades
Sensitivity 10 amol ATP
PHYSICAL CHARACTERISTICS
Connectivity One USB 2.0 port for computer control
Two USB 2.0 ports for printer connection and USB flash drive (touchscreen configurations only)
Power External 24VDC power supply compatible with 100-240 volts AC @50-60Hz. 
60W maximum consumption.
Dimensions 15” H x 15” W x 15” D (with touchscreen)
(38.1 cm H x 38.1 cm W x 38.1 cm D)
12” H x 15” W x 15” D
(30.5 cm H x 38.1 cm W x 38.1 cm D)
Weight < 27 lbs (12.3 kg)
REGULATORY
Regulatory CE and TUV marked. RoHS Compliant. Models for In Vitro Diagnostic use are available.
 
Absorbance
Fluorescence
Luminescence
Touchscreen
SLXA
 
 
 
SLXF
 
 
SLXFA
 
SLXATS
 
 
SLXFTS
 
SLXFATS
robotics


BioTek’s robotic instruments can help automate workflows in your laboratory. Many life science workflows benefit from expanded automation, for increased throughput or process efficiency. BioTek offers unique automation solutions that integrate with our microplate washers, dispensers, readers and imagers. BioStack is a compact stacker that offers fast plate exchange options for 50 plates or more, and patented plate de-lidding and re-lidding with BioStack 4 . BioSpa 8 Automated Incubator links BioTek readers or imagers together with washers and dispensers for full workflow automation of up to 8 microplates. Temperature, CO2/O2 and humidity levels are controlled and monitored through the BioSpa software to maintain an ideal environment for cell cultures during all experimental stages.
Take3


Micro-volume quantification is fast and easy with the Take3™ and Take3 Trio Micro-Volume plates, used in your BioTek microplate reader. Measure multiple 2 µL samples at a time, without diluting and without needing specialized equipment. Pre-programmed nucleic acid and protein protocols in Gen5 make quantification fast and easy.

  • Low volume 2 µL nucleic acid and protein quantification
  • Pre-programmed protocols in Gen5 Software
  • Absorbance and fluorescence detection
  • Easy, wipe-off maintenance
  • Read 2 µL microspots, BioCells or standard cuvettes
  • Gen5


    Gen5 software controls all BioTek automated microplate readers, configuring system and data collection parameters, analysis and a variety of reporting and exporting features. One-click export to Excel® makes it fast and easy to export displayed data.
    Gen5 Secure software is available for GxP compliant laboratories with features that support 21 CFR Part 11 rules.