BioTek Synergy 2

Multi-Mode Reader


The Synergy 2 modular multi-mode reader offers deep blocking filters and dichroic mirrors for the best level of performance in fluorescence detection. The monochromator-based, independent absorbance optical path makes UV-Vis applications fast, simple and precise. By using dedicated optical paths for each individual detection mode, Synergy 2 offers uncompromised performance and the lowest limits of detection.

• BioTek’s best price/performance ratio
• Fluorescence intensity, glow and flash luminescence, UV-Vis absorbance
• Time-resolved fluorescence, fluorescence polarization, Alpha detection
• 6- to 1536-well microplate compatible
• Take3 Plate compatible for micro-volume nucleic acid quantification

BioTek Synergy 2 Details

Measurement Modes
Fluorescense Intensity, Time-Resolved Fluorescence, Fluorescence Polarization, Alpha, Glow and Flash Luminescence, UV-Vis Absorbance, FRET, TR-FRET, BRET, Area Scanning, Spectral Scanning

Unique Optical System
A narrow band monochromator, optimized filter/dichroic mirror pairing, and three broad spectrum light sources achieve superior performance in all read modes

Compatible with Take3 Micro-Volume Plate:
Sixteen 2 µL microspots for low volume DNA/RNA 260 nm measurements. Saves precious samples, very fast and simple process

Low volume (2 µL) dsDNA quantification with Take3 Micro-Volume Plates
The Take3 Micro-Volume plate extends the Synergy H1 capabilities beyond standard microplate reading, by measuring up to 48, 2 µL samples at one time for rapid nucleic acid and protein quantification. Absorbance and fluorescence measurements are quick and easy, no additional instrumentation is required!

High Sensitivity:
Dedicated optical elements optimized for each specific modality result in the lowest limits of detection available in one compact instrument

Modular Design:
Purchase only what you need and easily upgrade later as experimental requirements expand

High Throughput:
Optimized for screening assays including 1536-well read mode

Wide Range of Applications:
With built-in temperature control, shaking, and optional dual reagent injector, virtually any microplate-based application is possible

Gen5™ Software:
Synergy 2 is driven by the most powerful and efficient microplate control, data collection and analysis software available today.
Detection modes UV-Vis absorbance 
Fluorescence intensity
Fluorescence polarization
Time-resolved fluorescence
Read methods End point, kinetic, spectral scanning, well-area scanning
Microplate types 6- to 1536-well plates
1- to 384-well (luminescence)
Other labware supported PCR plates, Petri and cell culture dishes
Take3 Micro-Volume Plates
Temperature control 4-Zone™ incubation to 65 °C; +0.2 °C at 37 °C
Shaking Linear
Software Gen5™ Data Analysis Software
Gen5 Secure for 21 CFR Part 11 compliance (option)
Automation BioStack and 3rd party automation compatible
Light source Xenon flash
Detector photodiode
Wavelength selection monochromator
Wavelength range 200 - 999 nm, 1 nm increment
Monochromator bandwidth 2.4 nm
Dynamic range 0 - 4.0 OD
Resolution 0.0001 OD
Pathlength correction Yes
Monochromator wavelength accuracy +2 nm
Monochromator wavelength repeatability +0.2 nm
OD accuracy <1% at 2 OD
<3% at 3.0 OD
OD linearity <1% from 0 to 3.0 OD
OD repeatability <0.5% at 2.0 OD
Stray light 0.03% at 230 nm
Reading speed (kinetic) 96 wells: 11 seconds
384 wells: 22 seconds
1536 wells: 42 seconds
Light source Tungsten halogen
Xenon flash (option)
Detector PMT
Wavelength selection Filters/ dichroic mirrors
Wavelength range Tungsten lamp: 300 - 700 nm (850 nm option)
Xenon lamp: 200 - 700 nm (850 nm option)
Dynamic range >6 decades
Sensitivity Top: Fluorescein 1 pM (0.2 fmol/well 96-well plate; 0.1 fmol/well 384-well plate)
Bottom: Fluorescein 5 pM (1 fmol/well 96-well plate; 0.5 fmol/well 384-well plate)
Reading speed (kinetic) 96 wells: 11 seconds
384 wells: 22 seconds
1536 wells: 42 seconds
Wavelength range 300 - 700 nm
Dynamic range >6 decades
Sensitivity 10 amol ATP (flash)
100 amol ATP (glow)
Light source Tungsten halogen
High energy DPR Xenon flash (optional)
Detector PMT
Wavelength selection Filters/dichroics
Wavelength range 400 - 700 nm (320 - 850 nm option)
Sensitivity 3 mP at 1 nM fluorescein
Light source Xenon flash
Detector PMT
Wavelength selection Filters/dichroics
Wavelength range Filters: 200 - 700 nm (850 nm option)
Sensitivity Europium 60 fM (12 amol/well 96-well plate; 6 amol/well 384-well plate)
Light source Tungsten halogen
Detector PMT
Wavelength selection Filters
Sensitivity 100 amol of biotinylated-LCK-P peptide, 25 µL/ well in 384-well plate
Read speed 2 minute (96-well plate)
Supported detection modes All modes
Number 2 syringe pumps
Supported labware 6- to 384-well microplates
Dead volume 1.1 mL, 100 µL with back flush
Dispense volume 5 - 1000 µL in 1 nm increments
Dispense accuracy +1 µL or 2%
Dispense precision <2% at 50 - 200 µL
Power 250 Watts max.
Dimensions 17"W x 17.5"D x 14.5"H
(43.5 x 44.5 x 37.3 cm)
Weight 60 lbs (27 kg)
Regulatory CE and TUV marked. ROHS compliant. In Vitro Diagnostic use models are available.
Synergy™ 2 Custom Configurations
Contact BioSPX for part #
Module Description
S Synergy 2 base model.  Includes temperature control to 65ºC, Shaking, and Gen5™.
L Luminescence
F Fluorescence Intensity top and bottom, Alpha detection.  Includes Tungsten-Halogen light source.
P Fluorescence Polarization.  Requires F module.
T Time-Resolved Fluorescence.  Requires F module.  Includes high energy DPR Xenon Flash Lamp.
A UV-Visible Absorbance (monochromator-based).  Includes SQ Xenon Flash Lamp.
D Dual Reagent Dispenser
Red shifted PMT (900nm) option available instead of standard low noise PMT (700 nm).
3 mm diameter bottom fiber options available instead of standard 5 mm bottom fiber.


BioTek’s robotic instruments can help automate workflows in your laboratory. Many life science workflows benefit from expanded automation, for increased throughput or process efficiency. BioTek offers unique automation solutions that integrate with our microplate washers, dispensers, readers and imagers. BioStack is a compact stacker that offers fast plate exchange options for 50 plates or more, and patented plate de-lidding and re-lidding with BioStack 4 . BioSpa 8 Automated Incubator links BioTek readers or imagers together with washers and dispensers for full workflow automation of up to 8 microplates. Temperature, CO2/O2 and humidity levels are controlled and monitored through the BioSpa software to maintain an ideal environment for cell cultures during all experimental stages.

Micro-volume quantification is fast and easy with the Take3™ and Take3 Trio Micro-Volume plates, used in your BioTek microplate reader. Measure multiple 2 µL samples at a time, without diluting and without needing specialized equipment. Pre-programmed nucleic acid and protein protocols in Gen5 make quantification fast and easy.

  • Low volume 2 µL nucleic acid and protein quantification
  • Pre-programmed protocols in Gen5 Software
  • Absorbance and fluorescence detection
  • Easy, wipe-off maintenance
  • Read 2 µL microspots, BioCells or standard cuvettes
  • Gen5

    Gen5 software controls all BioTek automated microplate readers, configuring system and data collection parameters, analysis and a variety of reporting and exporting features. One-click export to Excel® makes it fast and easy to export displayed data.
    Gen5 Secure software is available for GxP compliant laboratories with features that support 21 CFR Part 11 rules.