BioTek | Software


Gen5™ Data Analysis Software incorporates more than 30 years of experience and user feedback into an outstanding microplate reader software. Gen5 offers a unique combination of power and ease-of-use that drives productivity and saves time, no matter what application or workflow is incorporated. Gen5 seamlessly integrates with all BioTek imaging, multi-mode and single mode detection systems, providing control for all reader configurations in a simple setup interface.

Data analysis in Gen5 spans a very wide range of applications, from basic ELISA to high magnification image analysis with an interface designed specifically for all users. Endpoint, kinetic, spectral scanning, well area scanning and well imaging are all available based on the detection system, along with temperature control, reagent injection and stacking operations. Data output is completely configurable, from a quick export to Excel® button for the ultimate fast data transfer, to a completely customizable format for publication quality reporting.

Gen5 is one powerful software for microplate reading and imaging operations.

Multiple windows in Gen5 provide the full data picture

Gen5 is available in English, Chinese, Japanese and Russian editions.


All-User Friendly. Really!

Many software packages claim to be user-friendly, but Gen5's thoughtful design accommodates users at all levels for a wide range of applications, from basic endpoint ELISA quantification to in-depth analysis of high-magnification images. Using Gen5 can be as simple as clicking the "Read Now" button to start. When the data is returned, you can process, analyze, transform, crunch or otherwise manipulate it using Gen5’s data analysis tools. Basic or complex data is displayed in a clean, colorful way to facilitate data interpretation.

On the other hand, you can quickly export the data, in fact, Gen5 will even ask

Export Gen5 data

Along with the quick export function, you can use the amazingly customizable reporting and exporting options in Gen5’s Report/Export Builders to provide just the data you want, in the format you want…with publication-ready outputs. Special attention has been placed on result presentation so complex data can be displayed in a clean, colorful way to facilitate data interpretation.

Powerful Functionality

Gen5 includes powerful tools such as 4-P and 5-P curve fits with or without weighting, parallel-line analysis, advanced kinetic analysis, automated pathlength correction, multi-level data transformation, QC features like cut-off and validation criteria definition and calculation... and so much more. Used with Cytation 3 for cell imaging, Gen5 Image+ offers image analysis features typically only seen in dedicated imaging systems. Cell counting, subpopulation analysis and hit-picking processes are readily available .

Web-based Sample Files

A comprehensive searchable library of sample files with data is available, so there’s no need to spend time creating protocols for common (and not so common) applications! New files are added on a regular basis and Gen5 users can upload files to share their experiments and sample data with other Gen5 users.


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Liquid Handling Control™ (LHC™) Software is a powerful yet flexible interface for use with BioTek's 405™ Touch Washer, 405™ LS Washer, ELx405™ Select Deep Well Washer, EL406™ Washer Dispenser, and MultiFlo™ FX Dispenser. Full PC control provided by LHC Software leads to increased convenience when programming important assay-specific protocol requirements in a familiar Windows® environment. Any programming sequence possible onboard the washer or dispenser may be duplicated from the computer with LHC. With intuitive StepWise™ protocol creation, even novice users will find LHC Software easy to use.

21 CFR Part 11 compliance

To meet the demands of the GxP laboratory, LHC™ Secure offers features to ensure compliance to the U.S. FDA's 21 CFR Part 11 directive. Flexible multi-user permission levels and electronic protocol and system audit trail signing are all available whenever additional security is required.

SiLA Compliance

BioTek's LHC™ SiLA driver enables seamless integration of our industry leading washer and dispensers to automated systems conforming to international standards set forth by the Consortium for Standardization in Lab Automation (SiLA) creating open connectivity in lab automation.

Expanded versatility, Automatic protocol validation

LHC Software allows an unlimited number of methods to be linked together for the most complex liquid handling routines. From an instrument’s first prime routine, multiple microplate processes over time, an ultrasonic cleaning to dissolve protein or salt crystal build-up to a final system rinse, LHC Software enables unattended operation. Protocols are automatically validated to ensure feasibility and compatibility with the selected instrument.

Dual mode control

Dispense protocols can be created and run from the PC or downloaded to the instrument’s keypad. This is particularly useful when the liquid handler is installed in a biological safety cabinet. Protocols can be locked to avoid unintentional keypad modifications.

Custom maintenance reminders

To facilitate maintenance and keep a washer or dispenser in peak condition, factory recommended maintenance procedure reminders can be preset and customized appropriate for a busy laboratory’s usage and throughput requirements.

Safe record keeping

Protocol parameters may be printed for safe record keeping. Alternatively, onboard instrument protocols are able to be uploaded and backed up on a laboratory’s network. Satellite research labs working on joint projects can be certain their wash parameters are identical for experimental integrity.


For batch processing applications, LHC Software supports BioStack™ Microplate Stacker integrations. Robotic integration of BioTek washers or dispensers to commercially available robotic systems is also seamless with Liquid Handling Control.


* Full control of BioTek liquid handlers such as 405 Touch, 405 LS, ELx405 Select Deep Well, EL406, and MultiFlo FX
* Program assay-specific requirements in familiar Windows® environment
* Intuitive StepWise™ protocol creation with an unlimited number of methods included for ultimate flexibility
* Automatic protocol validation for error-free assurance
* Dual mode control – create and run protocols from PC or download to instrument’s keypad
* Security enabled protection for downloaded protocols
* Printed protocol reports
* Custom maintenance reminders for continued instrument performance in busy laboratories
* Multiple instruments controlled in parallel, including BioStack integrations for unattended batch processing
* LHC Secure additionally offers:
* Full compliance with the United States Food and Drug Administration’s 21 CFR Part 11 regulation
* User permission levels and authentication
* Time stamped protocol and system audit trails
* Embedded signatures
* Automatic protocol history
* Email alerts
* LHC SiLA provides drivers for integration into SiLA compliant automation platforms


LHC software offers PC control and third party robot system integration of 405 Touch / 405 LS / ELx405 Select Deep Well Washers, EL406 Washer Dispenser and MultiFlo / MultiFlo FX Dispensers. License for 5 installations.

LHC Secure software offers features to facilitate compliance to FDA directive 21 CFR Part 11 with PC control of 405 Touch / 405 LS / ELx405 Select Deep Well Washers, EL406 Washer Dispenser and MultiFlo / MultiFlo FX Dispensers. License for 5 installations.

LHC SiLA software driver allows for BioTek washer and dispenser integration into SiLA compliant automated systems. Includes LHC software for PC control and third party integration of 405 Touch / 405 LS / ELx405 Select Deep Well Washers, EL406 Washer Dispenser and MultiFlo / MultiFlo FX Dispensers. License for 5 installations.

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Precision Power™ is BioTek’s PC software for complete Precision™ control. The graphical user interface offers users powerful and flexible program creation under Microsoft Windows® 2000, XP and Vista (Professional editions). Virtually any liquid transfer routine can be programmed with Precision Power's open and intuitive format. Precision Power expands the instruments' dynamic capabilities with features such as a graphical program simulator, sample ID tracking and integration of up to two BioStack™ Microplate Stackers for automated delivery and retrieval of microplates to and from the Precision's platform.


PRCPWRV2 Precision Power software offers PC control and third party robot system integration of Precision / Precision XS Pipetting Systems. License for 2 installations.

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