BioTek PowerWave HT

Microplate Spectrophotometer


With super-fast plate reading speeds and a broad UV-Vis wavelength range, BioTek’s PowerWave HT Microplate Spectrophotometer is ideal for myriad applications, from direct quantitation of nucleic acids to full-scale high throughput operations that demand speed, accuracy and reliability. Rugged hardware, proven optical performance and a very small footprint, the PowerWave HT is uniquely designed to provide the most application flexibility while saving space both on the benchtop and in automated systems.

• 200 nm – 999 nm with no interference filters required
• Fast 96- and 384- well plate reading
• Superior 4-Zone™ temperature control
• Compact footprint well suited for automation
• Gen5 Microplate Reader and Software for data collection and powerful analysis

BioTek PowerWave HT Details

No Interference Filters Required
Monochromator optical design provides continuous wavelength selection from 200 nm to 999 nm. Measuring wavelengths can be selected in 1 nm increments to meet all assay requirements – it’s like having 800 filters built in! Spectral scanning capability allows quick determination of peak wavelengths in unknown solutions.

Microplate Types
96 and 384-well plates measured. In addition, the PowerWave HT can also read up to 8 of BioTek’s patented Bio-Cell™ quartz vessels for fixed 1 cm measurements.

Fast Reading Speed
Using “on the fly” technology, an entire 96-well plate can be read as few as 5 seconds

Temperature Control and Microplate Shaking
BioTek’s 4-Zone™ temperature control is a standard feature with the PowerWave HT. BioTek microplate readers use a natural convection system rather than a forced hot air system. This reduces evaporation in the microplate across its broad temperature range of 4° above ambient to 50°C. In the reading chamber, the microplate is completely surrounded by four independently controlled heaters ensuring low variation across the microplate. Users select from four shaking intensities and can define shake time for controlled agitation.

Robotics Compatible
The PowerWave HT's small footprint makes it ideal for robotic systems. The carrier is designed for easy robot gripper access. PowerWave HT is compatible with BioStack 3 and BioStack.

Gen5™ Data Analysis Software included with all models.
PowerWave HT is driven by the most powerful and efficient microplate control, data collection and analysis software available today.
Detection modes Absorbance
Read methods Endpoint, kinetic, spectral scanning
Microplate types 96- and 384-well plates
Temperature control 4-Zone™ incubation to 50 °C
+ 0.2 °C at 37 °C
Shaking Linear
Software Gen5™ Data Analysis Software
Gen5 Secure for 21 CFR Part 11 compliance (option)
Automation BioStack and 3rd party automation compatible
Light source Xenon flash
Detector photodiode
Wavelength selection monochromator
Wavelength range 200 to 999 nm, in 1 nm increments
Monochromator bandwidth 5 nm
Dynamic range 0 - 4.0 OD
Resolution 0.001 OD
Pathlength correction yes
Monochromator wavelength accuracy +2 nm
Monochromator wavelength repeatability +0.2 nm
OD accuracy 1% + 0.01 OD
OD linearity 1%
OD repeatability 0.5% + 0.005 OD
Stray light 0.03% at 230 nm
Reading speed (kinetic) 96 wells: 5 seconds
384 wells: 11 seconds
Power 100-240 Volts AC. 50/60 Hz.
Dimensions 8.5"W x 16"D x 8.5"H
(21.6 x 40.6 x 21.6 cm)
Weight 24 lbs (11 kg)
Regulatory CE and TUV marked. ROHS compliant. In Vitro Diagnostic use models are available.
PowerWave HT: 200-999 nm, 96- and 384-well plate reading with programmable shaking and temperature control from ambient +4°C to 50°C.

BioTek’s robotic instruments can help automate workflows in your laboratory. Many life science workflows benefit from expanded automation, for increased throughput or process efficiency. BioTek offers unique automation solutions that integrate with our microplate washers, dispensers, readers and imagers. BioStack is a compact stacker that offers fast plate exchange options for 50 plates or more, and patented plate de-lidding and re-lidding with BioStack 4 . BioSpa 8 Automated Incubator links BioTek readers or imagers together with washers and dispensers for full workflow automation of up to 8 microplates. Temperature, CO2/O2 and humidity levels are controlled and monitored through the BioSpa software to maintain an ideal environment for cell cultures during all experimental stages.

Gen5 software controls all BioTek automated microplate readers, configuring system and data collection parameters, analysis and a variety of reporting and exporting features. One-click export to Excel® makes it fast and easy to export displayed data.
Gen5 Secure software is available for GxP compliant laboratories with features that support 21 CFR Part 11 rules.