CEM | Microwave Peptide Synthesis


Overview

CEM Corporation is transforming the way chemists perform peptide synthesis once again with the introduction of the Liberty BlueTM automated microwave peptide synthesizer. More than just a facelift, the Liberty Blue system has been designed for maximized speed and efficiency. With 4-minute cycle times and a 90% solvent reduction, no other peptide synthesizer on the market can even come close to the performance of the Liberty Blue. Couple with that unparalleled peptide quality along with greater flexibility and improved reliability, Liberty Blue is the system of choice for all your peptide synthesis needs.

The Liberty Blue system's combination of microwave peptide synthesis along with rapid liquid handling offers several advantages over conventional synthesis conditions including:

* Shorter reactions resulting in only 4-minute cycle times
* Higher purity peptides than conventional synthesis
* Use of standard, inexpensive peptide synthesis reagents
* Synthesis of peptides impossible to synthesize under conventional conditions
* License included allowing the use of microwave energy for both the coupling and deprotection steps

Specifications

Synthesis Scale: 0.020 – 5 mmol
Reaction Vessel Sizes: 10, 30, and 125 mL standard
Chemistry: Fmoc only
Activation Method: In situ, standard activators
Amino Acid Reagents: 20 plus 7 additional positions
External Bottle Positions: 2 plus 7 additional positions
Temperature Measurement: In situ Fiber-optic
Fluid Measurement: Flex-Add - amino acids, activator, activator base Timed delivery – main wash, deprotection
Agitation: Programmable inert gas bubbling
Waste Container: 1 L reservoir with overflow detection
Controller: Laptop with wireless option standard
Reports: Printable pdf file for each method created. Log created during each run of every step performed
Power: 120V/60Hz or 240V/50Hz
Dimensions: 51 cm x 46 cm x 76 cm

License: The purchase of this instrument includes a grant of a limited, non-transferrable license to use this instrument for microwave peptide synthesis using microwave energy for both the coupling and deprotection steps.

Patents: US7393920; US7582728; US8058393; EP1491552; JP4773695, with additional worldwide patents pending

Advantages

Remarkably Fast

* 4 minute cycle time
* Rapid reagent addition
* Optimized synthesis methods
* >80% time reduction compared to 1st generation microwave synthesizers
* Difficult peptides such as A-beta in hours rather than days



Maximum Efficiency

* Up to 90% solvent reduction
* Less reagent usage
* No priming required
* Judicious use of solvent for washing
* Accurate and precise reagent addition

Benefits of Microwave Control

* Only system capable of using microwave energy for both the coupling and deprotection steps
* Complete control over power, temperature and time for each step
* Rapidly change temperature from one stage to the next
* Easily synthesize peptides with temperature sensitive amino acids without unwanted side reactions

Peptide Sequence: GEQKLGAib*AibASEEDLG-NH2


Flexibility

* Flex-add™ technology eliminates need for sample loops and allows infinite volume delivery options
* Synthesis conditions (temperature, power, and time) can be controlled for each step of every amino acid cycle
* 27 AA positions provides ample positions for unusual amino acids or reagents
* Unlimited number of couplings with amino acid stock solutions
* 3 reaction vessel sizes – 10, 30, and 125-mL to cover the full synthesis scale range
* Scale range from 0.02 to 5 mmol
* Resin loading option allows high throughput peptide synthesis
* Optional cleavage accessory for rapid peptide cleavage and deprotection

Simplicity

* 50% fewer processing valves
* Easy to use software with default methods
* Straightforward method customization for unique peptides or reagents
* Usage and reagent calculators make reagent preparation simple
* Fast and easy system setup with a maximum of only 31 bottles to install on the system

Reliability

* All valves are backpurged immediately after use eliminating the need for extra washing and preventing clogging issues
* Reduced routine maintenance with fewer valves and the elimination of optical sensors for reagent addition
* Flex-add technology is not susceptible for bubbles unlike optical sensor
* Uninterrupted pathway allows fast and efficient purging
* Pressure detection for all chemical transfers
* Detailed run history and error reporting Waste level detection
* Best in class service and support

Options

Optional Accessories

Resin Loading Accessory
High Throughput Microwave Peptide Synthesis

To increase the throughput of the Liberty Blue system an optional modular resin loading option is available that will allow up to 24 peptides to be synthesized sequentially. The resin loader comes in a 12-position module with an additional 12-position module that can be coupled to it. With the rapid 4-minute cycle time along with the potential to synthesize 24 peptides unattended overnight, the Liberty Blue system provides throughput of peptides that is comparable to that of conventional high throughput systems but with considerably better purity.

Advantages of Fast Sequential Peptide Synthesis

* Single Peptides Fast - 1 to 2 hours for standard length peptides
* Less Complex Instrumentation - easier to maintain and operate
* Eliminates Purification Bottleneck - purify peptides as soon as they are completed
* Precise Control at each Step - unique methods can be run for each peptide
* Higher Purity Peptides - reduces purification costs

Accent Peptide Cleavage Option
Isolate Peptides Cleanly and in a Fraction of the Time

The Accent Cleavage System is an isolated liquid handling module designed for performing the peptide cleavage step. The isolated module provides the advantage of protecting the Liberty Blue system from the harsh reagents required for the cleavage all while allowing the use of microwave irradiation to provide high quality peptides in excellent yields in a fraction of the time.

* Full peptide cleavage in 30 minutes or less
* Microcleavage in as little as 2 minutes
* Improved peptide purity
* Increased peptide yield

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Overview

Developer of the first microwave peptide synthesis system, CEM is the leader in the field and we continue to expand the scope of this technology to provide systems to satisfy the needs of all peptide chemists. The Liberty XL is a mid-scale microwave peptide synthesizer capable of preparing hundreds of grams of peptide in an automated fashion with all the speed and efficiency of microwave technology. Using the Liberty XL, chemists can now quickly generate enough peptide material to fulfill further testing or for a pre-clinical trial in high purity and yield.

Specifications


Synthesis Scale 10 – 100 mmol
Reaction Vessel Sizes: 2.5 L
Chemistry: Fmoc only
Activation Method: In situ or pre-activation, standard activators
Amino Acid Reagents 20 (1 L capacity)
External Bottle Positions: 4
Temperature Measurement: In situ Fiber-optic
Fluid Measurement: Timed delivery, One shot option
Fluid Transfer: Positive nitrogen pressure for amino acids, Independent pumps for each external position and RV drain
Agitation: Mechanical
Waste Container: Variable reservoir with overflow detection
Controller: User selectable desktop or laptop with wireless option standard
Reports: Printable pdf file for each method created. Log created during each run of
every step performed
Power: 200/208/230 VAC (200-253 VAC), 60 Hz, 15A @ 230 VAC 220/240 VAC (202-250 VAC), 50 Hz, 15A @ 240 VAC
Dimensions: 121.9 cm x 73.7 cm x 162.6 cm
License: The purchase of this instrument includes a grant of a limited, non transferrable license to use this instrument for microwave peptide synthesis using microwave energy for both the coupling and deprotection steps.
Patents: US7393920; US7582728; US8058393; EP1491552; JP4773695, with additional worldwide patents pending

Advantages

The Microwave Advantage

* License included with each system allowing the use of microwave energy for both the coupling and deprotection steps
* Fastest cycle times available Unparalleled peptide purity and yield compared to conventional synthesis
* No concern for thermal run-away with instant on/off power control
* Access to peptides impossible to synthesize under conventional conditions



Flexibility

* 2.5 L reaction vessel providing a synthesis scale range up to 100 mmol
* 20 amino acid positions with either aliquot or full volume delivery
* Fully automated operation or semi-automated for critical synthesis steps
* Easily transfer methods from Liberty Blue system
* Simple vessel design makes resin transfer and clean up easy

Speed and Efficiency

* Cycle time of less than 30 minutes
* Direct and accurate temperature control with fiber optic probe
* Inert atmosphere for reagents and reaction vessel
* Efficient washing and agitation

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