BioTek MultiFlo FX

Multi-Mode Dispenser

Overview

MultiFlo™ FX is an automated reagent dispenser for 6- to 1536-well microplates. Its unique Parallel Dispense™ technology allows up to four independent reagents to be dispensed in parallel without potential carryover. What makes MultiFlo FX “multi-mode” is its configurability: you can add a wash module or the RAD™ module, for random access individual well dispensing and bulk dispensing larger wells. Everything you need for fast, intuitive programming and operation is available through the color touchscreen. MultiFlo FX configured with either the wash module or RAD technology replaces up to five liquid handlers in a single compact platform.

• Multi-mode dispensing can replace up to four dispensers and a washer
• Parallel Dispense™ technology: peristaltic and/or syringe pumps
• RAD™ technology for dispensing to individual wells
• Cost savings through low prime volume and single tube access
• Cell friendly angled dispense and/or wash tubes won’t disrupt cell layers
• Walkaway automation with BioStack 4, for lidded or unlidded plates

MFX
MultiFlo FX Dispenser product video.

BioTek MultiFlo FX Dispenser



Multi-mode dispensing can replace up to four dispensers and a washer
Providing unsurpassed liquid handling flexibility, MultiFlo FX’s modular base can accommodate 1 or 2 peristaltic pumps, a 2-syringe pump module and a wash module or a single channel dispense. MultiFlo FX takes the place of multiple instruments, but is compact and very simple to use.

Parallel Dispense™ technology: peristaltic and/or syringe pumps
Peristaltic pumps and syringe pumps each offer benefits; peri-pumps help save precious reagent, while syringe pumps are fast and precise. MultiFlo FX can be configured with 2 peri and 2 syringe pumps, allowing up to four reagents to be dispensed in parallel, without carryover.

RAD™ technology for dispensing to individual wells
When the liquid handling workflow calls for individual well dispensing along with rapid bulk dispensing, MultiFlo FX can handle it. The unique RAD module provides single channel dispensing to discrete wells of a 48-, 96- or 384-well plate. For rapid single channel dispensing to 6- to 24-well plate, the 8-to-1 RAD cassette is perfect for the task.

Cost savings through low prime volume and single tube access
With programmable prime volumes, backflush capability and the unique single tube access with RAD, you’ll save on costly reagents for all your dispensing processes.

Cell friendly angled dispense and/or wash tubes won’t disrupt cell layers
For sensitive cell-based assays, the dispense tips on the MultiFlo FX dispense manifolds and wash manifolds are angled, allowing gentle reagent dispense down the well sides. This helps keep cell layers intact, even through multiple-step operations.

Walkaway automation with BioStack 4 or BioSpa 8 for lidded or unlidded plates
Integration BioStack to MultiFlo FX allows automated processing of multiple plates at a time, without manual intervention. MultiFlo FX integrated to BioSpa 8 adds automated media exchange and reagent addition to automated live cell workflows.
GENERAL
Microplate types 96-, 384- and 1536-well
Low profile and standard height
Solid and filter bottom (option)
Onboard software Create, edit or run multiple protocols
Software (computer control) LHC2 Software
LHC2 Secure for 21 CFR Part 11 compliance (option)
SiLA Compliant driver (option)
Separation Biomagnetic separation, vacuum filtration (optional)
Shaking Programmable up to 60 minutes
Slow, medium, fast or variable
Soaking Programmable up to 60 minutes
Ultrasonic Advantage™ Standard on most configurations
Automation BioStack and 3rd party automation compatible
BioSpa 8 Automated Incubator compatible
WASHING
Manifold types 96-well washing: 96-tube manifold - straight dispense tubes
96- and 384-well washing: 96-tube Dual-Action manifold - 20° angled dispense tubes
384-well washing (fast): 192-tube Dual-Action manifold - 7° angled dispense tubes
1536-well washing: Two 32-tube dispense manifolds - 7° angled dispense tubes
Volume range 3 - 3,000 µL/well in 1 µL increments
Wash cycles 1 - 250
Buffer/reagent selection Auto switching module for up to 4 buffers (option)
Supply bottle 4 L or 10 L (optional)
Dispense accuracy 3%
Dispense precision <3% CV (model dependent)
Residual volume <2 µL/well
Wash speed 96 wells, 300 µL/well, 96-tube manifold: 13 seconds 
384 wells, 100 µL/well, 192-tube manifold: 17 seconds 
1536 wells, 10 µL/wells, two 32-tube manifolds: 36 seconds
Flow rates High flow to low flow 
Optimized rates for cell assays
Sterilization Chemical
Vacuum range for filtration Selectable: 0 to -380 mmHg (final at 30s)
Filtration time: 5 - 999 seconds
DISPENSING - PERISTALTIC PUMP (MULTI-CHANNEL)
Manifold types 8-tip (1 x 8) cassette with plastic, 316 SS tubes or sapphire jeweled 316 SS tubes
Fluid delivery 1 or 2 peristaltic pumps
Dispense speed 96 wells, 10 µL /well: 8 seconds
384 wells, 5 µL /well: 12 seconds
1536 wells, 1 µL /well: 27 seconds
Volume range 500 nL - 3,000 µL/well, selectable in 1 µL increments
Flow rates User programmable rates from high to low
Optimized rates for cell assays
Dispense performance 1 µL cassette: 
Recommended Volume Range: 1 - 50 µL
Dispense Accuracy: +5% at 1 µL
Dispense Precision: <5% CV at 1 µL
<10% CV at 500 nL
Minimum Prime Volume: 1.20 mL
5 µL cassette:
Recommended Volume Range: 5 - 2,500 µL
Dispense Accuracy: +2.0% at 5 µL
Dispense Precision: <2.5% CV at 5 µL
Minimum Prime Volume: 4.23 mL
10 µL cassette:
Recommended Volume Range: 10 - 3,000 µL
Dispense Accuracy: +2.0% at 10 µL
Dispense Precision: <2.0% CV at 10 µL
Minimum Prime Volume: 7.36 mL
Recommended cassette replacement interval 1 µL Cassette: 1,000 384-well microplates at 5 µL well
5 µL Cassette: 1,000 96-well microplates at 50 µL well
10 µL Cassette: 1,000 96-well microplates at 100 µL well
Sterilization Autoclave, chemical
DISPENSING - SYRINGE PUMP
Manifold types 96-well dispensing: 
One 16-tube (2 x 8) manifold - 316 SS tubes
96-/384-well dispensing:
Two 16-tube (1 x 16) manifolds - 316 SS tubes
1536-well dispensing:
Two 32-tube (1 x 32) manifolds - sapphire jeweled 316 SS or 316 stainless steel tubes
Fluid delivery Two positive displacement syringe drives
Dispense speed 20 µL /well, 96 wells, 1 x 16 tubes: 5.25 seconds
20 µL /well, 384 wells, 1 x 16 tubes: 14 seconds
3 µL /well 1536 wells, 2 x 32 tubes: 7 seconds
Volume range 3 - 3,000 µL/well, selectable in 1 µL increments
Minimum Prime Volume: 12 mL
Flow rates User programmable rates from high to low
Dispense accuracy +1 µL at 5 µL
+1 µL at 20 µL
+1% at 100 µL
Dispense precision <5% CV at 5 µL
<2.5% CV at 20 µL
<1% CV at 100 µL
Supply bottle 1 L or 2 L
Sterilization Chemical, autoclavable option
PHYSICAL CHARACTERISTICS
Power 100 - 240 Volts AC. 50/60 Hz
Dimensions 16.5" W x 18" D x 12.5" H (42 x 46 x 32 cm)
Weight 32 lbs (14.5 kg)
REGULATORY
Regulatory CE and TUV marked. ROHS compliant. In Vitro Diagnostic use models are available.
 
MFX
MFXP*
MFXW**
MFXPW**
MFXR***
MFXPR***
Unconfigured base instrument
         
1 peripump dispenser only  
       
Wash module only    
     
1 peripump dispenser and wash module      
   
RAD technology with one peripump        
 
RAD technology with two peripumps          


*P configurations: include one plastic tip cassette
**W configurations: include 96/384 wash manifold
***R configurations: include one plastic tip multi-channel or RAD cassette.


Dispenser Modules (purchased separately)
7210010 - External Peristaltic Pump Dispenser Module
Adds 1 peristaltic pump dispenser to MultiFlo FX base instruments. For 6- to 1536-well microplates. Includes 1 plastic tip cassette.
Note: 7210010 is not required and is not compatible with MFXR or MFXPR

7210008 - External Dual Syringe Dispenser Module, Autoclavable
Adds 2 syringe drive dispensers to MultiFlo FX base instruments.

7210009 - External Dual Syringe Dispenser Module, Non-Autoclavable
Adds 2 syringe drive dispensers to MultiFlo FX base instruments.

LHC


The MultiFlo FX extensive onboard software, accessed through the convenient touchscreen interface. With an intuitive menu-driven interface. Program all aspects of the system’s dispense, wash and maintenance parameters, quickly and easily.

LHC
LHC Software allows computer-based programming of multi-step processes and it is used for integrating BioTek’s multi-mode washer dispensers, washers and dispensers into robotic systems. You can operate the system under computer control via LHC, or create protocols in LHC and download to the instrument for recall and execution.

LHC Secure software
LHC Secure software offers features to facilitate compliance to FDA directive 21 CFR Part 11
with computer control of BioTek’s Multi-Mode Washer Dispensers, Washers and Dispensers.

LHC SiLA software
LHC SiLA softwaredriver enables BioTek washer and dispenser integration into SiLA compliant automated systems. Includes LHC software for computer control and third party integration of BioTek’s Multi-Mode Washer Dispensers, Washers and Dispensers.