BioTek ELx405 Select

Deep Well Washer


The ELx405 Select is the world’s first deep well microplate washer that aspirates and dispenses into 96- and 384-well plates. ANSI/SBS standard height microplates can also be washed, making the ELx405 Select a total solution for all your laboratory’s microplate washing workflows. The ELx405™ Select is automation compatible and its patented Dual-Action™ manifold is particularly useful in cell-based assays. Maintenance is easy with the available patented Ultrasonic Advantage™ that automated cleans manifold tubes to remove built-up salt and protein. The ELx405 Select is ideal for all standard and deep well microplate washing requirements.

• Washes 96- and 384-well deep well and standard height plates
• Ideal for cell culture, plasmid DNA purification and much more

BioTek ELx405 Select Deep Well Microplate Washer Details

Washes 96- and 384-well deep well and standard height plates
The ELx405 Select is the premier microplate washer accommodating deep well blocks and standard height microplates, in 96- and 384-well format, without requiring specialized manifolds.

Ideal for cell culture, plasmid DNA purification and much more
Wash processes used in cell culture and plasmid DNA preparation are automated with the ELx405 Select – there’s no need for specialized instrumentation. ELx405 Select can also process magnetic and polystyrene bead protocols, making it a versatile plate washer for many laboratories

Microplate types 96- and 384-well 
Low profile and standard height
Deep well plates up to 50 mm
Onboard software Create, edit or run multiple protocols
Software (computer control) LHC2 pc software
LHC2 Secure for 21 CFR Part 11 compliance (option)
SiLA Compliant driver (option)
Shaking Programmable up to 60 minutes
Slow, medium, fast or variable
Soaking Programmable up to 60 minutes
Automation BioStack (for standard plate heights only) and 3rd party automation compatible (deep well and standard plates)
Manifold types 96-tube Dual-Action manifold for 96- & 384-well washing
Volume range 50 - 3000 µL/well, in 1 µL increments
Wash cycles 1-okt
Buffer/reagent selection Auto switching module for up to 4 buffers (option)
Supply bottle 4 L or 10 L (optional)
Dispense precision ≤3% CV: 300 µL/well (96-well washing)
Residual volume <2 µL/well (standard & deep well 96-well plates)
Wash speed 96-wells, 300 µL/well, 3 cycles: <30 seconds
384-wells,100 µL/well, 3 cycles: <80 seconds
Flow rates High flow to low flow
Optimized rates for cell assays
Sterilization Chemical
Vacuum filtration Selectable: Approximate final at 30 seconds:
Lowest: -38 mmHg
Low: -113 mmHg
Medium: -200 mmHg
High: -390 mmHg
Highest: -506 mmHg
Vacuum filtration time range: 5 to 999 seconds
Power 100 - 240 Volts AC. 50/60 Hz
Dimensions 14" W x 17" D x 11" H
(35.6 x 43.2 x 27.9 cm)
Weight 30 lbs (13.5 kg)
Regulatory CE and TUV marked. In Vitro Diagnostic use models are available.
Part #
96-/384- well
Deep Well
Standard Height
Cell Washing
Buffer Switching
Biomagnetic Separation*
* With available accessory module sold separately

ELx405™ Select Deep Well Microplate Washer Configurations
Feature Design
S Ultrasonic Advantage™ Built-in ultrasonic cleaner for manifold self-maintenace
V Automatic 4-Buffer Switching
U 96-/384-well microplate washing 96-tube (8 x 12) manifoldDual-Action™
D Deep well microplates Accomodates ANSI/SBS standard and deep well microplates smaller than 50 mm in height
CW Optimized cell washing for loosely adherent monolayers

The 405 LS has extensive onboard software, accessed through the convenient touchscreen interface. Program all aspects of the system’s wash and maintenance parameters, quickly and easily and store them onboard for routine use.

LHC Software allows computer-based programming of multi-step processes and it is used for integrating BioTek’s multi-mode washer dispensers, washers and dispensers into robotic systems. You can operate the system under computer control via LHC, or create protocols in LHC and download to the instrument for recall and execution.

LHC Secure software
LHC Secure software offers features to facilitate compliance to FDA directive 21 CFR Part 11
with computer control of BioTek’s Multi-Mode Washer Dispensers, Washers and Dispensers.

LHC SiLA software driver enables BioTek washer and dispenser integration into SiLA compliant automated systems. Includes LHC software for computer control and third party integration of BioTek’s Multi-Mode Washer Dispensers, Washers and Dispensers.