BioTek Cytation C10

Confocal Imaging Reader

Overview

The Cytation™ C10 Confocal Imaging Reader combines spinning disk confocal and automated digital widefield microscopy, plus a multi-mode microplate reader. This allows for an improved imaging quality and better cell analysis in a cost-effective manner. The C10 follows up on the previous Cytation™ equipment and is developed through gained expertise and user-feedback from the past models. Along with the Gen5 software, the Cytation™ C10 provides an easy and automated workflow from image capture, processing, data analysis to publishing.

• Combined spinning disk confocal, widefield microscopy and optional multi-mode microplate reader in one compact platform!
• High quality optical components
• Hit-picking: Multi-mode detection + imaging saves time and data storage
• Variable bandwidth monochromators for sensitivity and specificity
• Maintaining a consistent environment through CO2/O2 and temperature control
• Label-free cell counting for cell proliferation studies



Configurations

Part # C10PHC2 C10MPHC2 C10PWC C10MPWC C10PW C10MPW
Confocal 60 µm spinning disk
   
Confocal 40 µm spinning disk
 
     
Hamamatsu sCMOS camera
       
Sony CMOS camera
 
 
Multi-mode reading
 
 
 
Phase contrast

Video


BioTek Cytation C10 Details



Combined confocal and widefield microscopy
Image quality is considerably improved due to the combination of spinning disk confocal and widefield microscopy. The degree of detail is exceptionally enhanced, and this results in improved quantification and analysis of the assay, combined with the Gen5 imaging Software.

Widefield vs Confocal


High quality optical components
To enable high quality images and publication-ready images, high standard objectives and filters are compatible with the Cytation C10. These high quality objectives and filters include the Hamamatsu sCMOS Orca, Semrock filters, Olympus objectives and other known brands.

Cytation 10 components


Hit-picking: Multi-mode detection + imaging saves time and data storage
The plate reader screens all wells and identifies the wells that meet certain criteria. Only the wells that meet these, will be imaged by the microscope. This results in the saving of time and computer memory!

Cytation 10 Hitpicking
(1) Plate reader quickly identifies GFP positive wells.
(2) Only GFP positive wells are imaged, saving both time and computer memory.


Variable bandwidth monochromator for sensitivity and specificity
The bandwidths of the monochromators in the Cytation C10 fluorescence detection module is selectable from 9 nm to 50 nm, in 1 nm increments. Variable bandwidths allow you to optimize detection conditions for fluorophores that have unique excitation or emission parameters, for example, a narrow Stokes shift. Adjusting the bandwidth results in better performance than conventional monochromators.

Cytation 10 Bandwith
The plate reader optics of Cytation use a quad monochromator design with variable bandwidth. The bandwidth can be set anywhere between 9 and 50 nm in 1 nm increment. Large bandwidth settings (1) provide increased sensitivity and lower limits of detection. Small bandwidth settings (2) provide increased specificity when multiple signals are present, which reduces signal crosstalk and enhances assay performance


Maintaining a consistent environment through CO2/O2 and temperature control
Cytation C10 is well suited for live cell assays, with temperature control to 45 °C, plus linear and orbital shaking. A gas controller module is available to help regulate CO2 and O2 levels in the system for longer-term live cell requirements. A dual reagent injector provides quick inject/image capability for observing fast reactions.

Cytation 10 Temp. Control
Successful live cell kinetic imaging relies on a consistent environment, including temperature control and CO2/O2 control and monitoring. Cytation C10 provides the perfect environment to grow and analyze live cells over time. Powerful movie maker and kinetic analysis software tools allow visualizing and analysis time-lapse experiments.


Label-free cell counting
It’s fast and convenient to count live cells using the available high contrast brightfield option. Higher contrast allows a direct and label-free method for object identification using Gen5 software to count cells and characterize cell proliferation in kinetic studies.

GENERAL
Detection modes UV-Vis absorbance
Fluorescence intensity
Luminescence
Read methods Endpoint, kinetic, spectral scanning, well area scanning
Microplate types Monochromator: 6- to 384-well plates
Imaging: 6- to 1536-well plates
Other labware supported Microscope slides, Petri and cell culture dishes, cell culture flasks (T25), counting chambers (hemocytometer), Take3 Micro-volume plates
Temperature control 4-Zone incubation to 45 °C with Condensation Control
Shaking Linear, orbital, double-orbital
Software Gen5 Microplate Reader and Imager Software included
Gen5 Secure for 21 CFR Part 11 compliance (option)
Gen5 Image+ and Image Prime software available for full image analysis (option)
Automation BioStack and 3rd party automation compatible
CO2 and O2 control (option) Range: 0 - 20% (CO2); 1 - 19% (O2), with optional Gas Controller
Models for both CO2 and O2 or CO2 only are available
IMAGING - CONFOCAL MICROSCOPE
Imaging modes Fluorescence
Image processing Z-projection, digital phase contrast, stitching
Camera Hamamatsu Orca sCMOS, 16-bit grayscale camera or
Sony CMOS 16-bit grayscale camera
Objective capacity 6-position automated turret for user-replaceable objectives
Objectives available 20x, 40x, 60x
Image filter cube capacity 4 user-replaceable fluorescence cubes
Imaging filter cubes available CFP, CY5, DAPI, GFP, RFP, TRITC, brightfield
Laser 6-line
Automated functions Autofocus, user-trained autofocus, autoexposure, auto-LED intensity
Autofocus method Image-based autofocus
User-trained autofocus
Laser autofocus (option)
Positional controls Software Control
Joystick controller (option)
Imaging methods Single color, multi-color, time lapse, montage, z-stacking, z-stack montage
Field of view Hamamatsu sCMOS: 0.65 mm + 5% at 20x magnification
Sony CMOS: 0.70 mm + 5% at 20x magnification
Image collection rate Laser autofocus, 0 ms delay, 96 wells: 8 mins, 9 secs
Software autofocus, 0 ms delay, 96 wells: 12 mins, 1 sec
IMAGING - WIDEFIELD MICROSCOPE
Imaging mode Fluorescence, phase contrast, color brightfield, user-selectable brightfield/high contrast brightfield
Imaging method Single color, multi-color, time lapse, montage, z-stacking, z-stack montage
Image processing Z-projection, digital phase contrast, stitching
Camera Hamamatsu Orca sCMOS, 16-bit grayscale camera or
Sony CMOS 16-bit grayscale camera
Objective capacity 6-position automated turret for user-replaceable objectives
Objectives available 1.25x, 2.5x (2.25x eff), 4x,10x, 20x, 40x, 60x
Phase objectives available 4x, 10x, 20x, 40x
Image filter cube capacity 4 user-replaceable fluorescence cubes, plus brightfield channel
Imaging filter cubes available DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7, Acridine Orange, CFP-FRET, CFP-YFP FRET, Chlorophyll, Phycoerythrin (PE), Propidium Iodide, CY5.5, TagBFP, Tag BFP-FRET, GFP (Ex)-CY5 (Em), RFP (Ex)-CY5 (Em), Alexa 568, Ex 377/Em 647, Oxidized roGFP2, TRITC
Imaging LED cubes available 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 554 nm, 590 nm, 623 nm, 655 nm, 740 nm
Automated functions Autofocus, autoexposure, auto-LED intensity
Autofocus method Image-based autofocus
User-trained autofocus
Laser autofocus (option)
Positional controls Software Control
Joystick controller (option)
Image collection rate Image-based autofocus:
96 wells, 1 color (DAPI), 4x, 6 minutes
Laser autofocus:
96 wells, 1 color (DAPI), 4x, <3 minutes
Image Analysis Software option Gen5 Image+: Image analysis
Gen5 Image Prime: Advanced image analysis
Gen5 Secure: 21 CFR Part 11 compliant features
FLUORESCENCE INTENSITY
Light source Xenon flash
Detector PMT
Wavelength selection Quad monochromators (top/bottom)
Wavelength range 250 – 700 nm (900 nm option)
Monochromator bandwidth Variable, from 9 nm to 50 nm in 1 nm increments
Dynamic range 7 decades
Reading speed (kinetic) 96 wells, sweep mode: 10 seconds
384 wells, sweep mode: 20 second
LUMINESCENCE
Wavelength range 300 - 700 nm
Dynamic range >6 decades
ABSORBANCE
Light source Xenon flash
Detector Photodiode
Wavelength selection Monochromator
Wavelength range 230 - 999 nm, 1 nm increments
Monochromator bandwidth 4 nm (230 - 285 nm), 8 nm (>285 nm)
Dynamic range 0 - 4.0 OD
Resolution 0.0001 OD
Pathlength correction Yes
Monochromator wavelength accuracy + 2 nm
Monochromator wavelength repeatability + 0.2 nm
OD accuracy <1% at 3.0 OD
OD linearity <1% from 0 to 3.0 OD
OD repeatability <0.5% at 2.0 OD
Stray light 0.03% at 230 nm
Reading speed (kinetic) 96 wells: 10 seconds
384 wells: 20 seconds
REAGENT INJECTORS (OPTION)
Supported detection modes All modes
Number 2 syringe pumps
Supported labware 6- to 384-well plates, Petri and cell culture dishes
Dead volume 1.1 mL, with back flush
Dispense volume 5 – 1000 µL in 1 µL increments
Plate geometry 6- to 384-well microplates
Dispense accuracy +1 µL or 2%
Dispense precision < 2% at 50 - 200 µL
PHYSICAL CHARACTERISTICS
Power Instrument: External 250W (minimum), 24VDC power supply, compatible with 100-240 VAC @50-60Hz.
Optional 6-channel laser light source: External 250W power supply, compatible with 100- 240VAC @ 50-60Hz.
Optional Hamamatsu scientific camera: External 75W power supply, compatible with 100- 240VAC @ 50-60Hz.
Dimensions 27" W x 18.5" H x 20" D
(68.6 cm x 45.72 cm x 50.8 cm)
Weight 122 lbs (53.3 Kg)
REGULATORY
Regulatory CE and TUV marked. RoHS Compliant. Models for In Vitro Diagnostic use are available.
gen5 imaging software


From whole organism imaging to high magnification subcellular image analysis, Gen5 helps transform raw data and images into meaningful results. Gen5 Software automates image capture from BioTek’s Lionheart Automated Microscope and Cytation Cell Imaging Multi-Mode Reader, and automates image processing and analysis to produce publication-ready images and quantitative data. This process of Augmented Microscopy™ enables simple, straightforward imaging workflows.

• Automated image capture
• Exceptional image processing tools
• Powerful image analysis
• Publication-ready images, graphs and data


Features


Augmented Microscopy automates image capture for samples with powerful tools for endpoint and time lapse workflows. Fluorescence, brightfield, color brightfield and phase contrast images are captured in slides, microplates, chamber slides, cell culture dishes for quantitative and qualitative analysis.

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Exceptional Image Processing Tools
Gen5’s image processing tools provide exceptional processing capability to facilitate the analysis of complex biologies, including 3D samples, large montaged samples and processes including live cell kinetics. From easy brightness/ contrast adjustments to deconvolution and automated movie file creation, image processing in Gen5 enables optimized visualization and analysis for many applications. The independent optical paths ensure uncompromised performance in all modes


Powerful Image Analysis
Image analysis tools in Gen5 cover a very broad range of application requirements, and are both powerful and easy to use. Analysis functions in Gen5 extend to quantitative data as well. Cell count, confluence, signal translocation, subpopulation analysis and kinetic analysis are just some of the powerful data provided by Gen5’s image analysis tools.


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Publication-ready Images, Graphs and Data
Augmented Microscopy tools include the ability to create publication-ready images, graphs and data using the functions in Gen5 software. There is no need to export images or data to external software to create scatterplots, histograms, EC50 graphs and other data displays for publication.


Gascontroler


Gas Controller for CO2 and O2 controll
Increasingly, life science assays are run on live cells. Some of these assays require control over CO2 and O2 concentrations to modulate the environment for pH buffering or to create a hypoxic condition. These assays are traditionally run-in flasks in a low-throughput manner, but there is a burgeoning interest in automation of live cell-based assays in microplates for higher throughput and efficiency, therefore there is a need for cell-friendly microplate instrumentation.

The Gas Controller allows full control over CO2 and O2 concentrations to regulate the environment for microplate based live cell assays.